Parthenium-Turmeric ointment: A novel approach for excision wound healing on rabbits (original) (raw)
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Formulation and Evaluation of Polyherbal Wound Healing Ointment
International Journal of Pharma Research & Review, 2014
Aim: The present investigation was aimed to formulate and evaluate the polyherbal wound healing ointment constituting the methanolic extracts of Lantana camara (leaves), Tamarindus indicus (leaves), Psidium guajava (leaves) and acetone extract of Curcuma longa (rhizomes). Materials and Methods: Two Formulations (F1, F2) were formulated by using fusion method using a mixture of PEG 4000 and PEG 600 as ointment base. The physical evaluation of these polyherbal formulations were made in terms of physical and chemical parameters like pH, physical stability, centrifugation, spreadability, viscosity, extrudability. Assessment of wound healing activity was made by using excision wound model in which the evaluation parameters were wound contraction and epithelialization period in excision wound model by using healthy Albino wister rats. In this model, the animals were divided into 4 groups (n = 6) named as control, standard, formulation1 (F1), formulation2 (F2) receiving vehicle (ointment b...
DEVELOPMENT OF WOUND HEALING HERBAL FORMULATION
The present study was to evaluate the wound healing activity of developed polyherbal formulation in the form of ointment by using hydroalcoholic extracts of leaves of Ficus religiosa, Mentha arvensis and roots of Rauwolfia serpentina with excipients. The phytochemical studies revealed the presence of alkaloids, phytosterol, tannins, flavonoids and protein. The developed polyherbal ointment was examined for wound healing activity in two types of wound models on rats; the excision and incision wound model. The polyherbal formulation was applied once daily until complete healing of wound and it was noted that the period of epithelization significantly (P < 0.05) reduced when compared with vehicle control group (15 ±0.34) which was nearly comparable with standard drug group. The tensile strength of healed wound was significantly increased (P < 0.05) as compared to vehicle control group. Hence the developed polyherbal formulation showed significant (P < 0.05) wound healing activity as compared to control group. The obtained results were nearly compared with standard drug group betadine ointment in terms of contractibility, wound closure time and tensile strength.
Background: Wounds are considered health indispositions with detrimental socio-economic influences on the afflicted and their kin. Curcuma longa or turmeric has been used in the treatment of wounds. Employing the extracts of turmeric as a bioactive ingredient in an aqueous cream could enhance its value in wound treatment. Aim: This study aimed to formulate aqueous creams containing concentrations of ethanolic extract of turmeric (EET) as bioactive ingredients, evaluate their stability and wound healing activities in male Wistar rats using hydroxyproline (HXP) as a biochemical marker. Materials and methods: Solutions of 1.5, 3.0 and 5.0 % w/v of crude EET were prepared and also incorporated as bioactive ingredients in aqueous creams. The stability of the creams was evaluated and their wound healing effects were studied using distilled water, dimethyl sulphoxide (DMSO) and cholesterol as controls in male Wistar rats. Results: The creams were stable in colour, pH, viscosity, etc. and exhibited wound healing activities. The animals treated with the crude 1.5 % w/v EET and its cream demonstrated the highest tissue HXP level showing significant percentage differences in tissue HXP levels from the control groups (p < 0.05). Conclusion: The crude EET has been confirmed to possess wound healing properties with an optimal effective concentration for wound healing in male Wistar rats determined as 1.5 % w/v which when incorporated as a bioactive ingredient in an aqueous cream retained its efficacy in wound healing and could therefore be beneficial in the treatment of body injuries.
International Journal of Current Research and Review
Objective: The purpose of this study was to identify differences in wound healing and wound healing time at each concentration of turmeric extract gel (Curcuma longa Linn) and base gel on days 3, 7 and 14, and identify the most effective turmeric extract gel for wound healing in cuts female rats (Rattus Novergicus). Method: This study was an experimental laboratory using a post-test only with a control group. This study used 16 female rats, 3 treatment groups, and 1 control group (each group consisted of 4 mice). Female rats were injured in the back section by slashing along 1.5 cm with a depth of 0.2 cm. The treatment group was given turmeric extract gel with a concentration of 5%, 10%, 15%, and the control group was only given a gel base. This research was conducted for 14 days of topical turmeric extract gel by giving 2 times a day. The normality test uses the Shapiro Wilk test, then the Kruskal Wallis test, and continued with the Post-Hoc Mann-Whitney. Results: The mean on the 3rd day of the EG group was 5% (0.95cm), the EG group was 10% (0.98 cm), the group was 15% (1.23 cm), and the control group (1.25 cm). On the 7th day EG 5% (0.58 cm), EG 10% (0.65 cm), EG 15% (0.73 cm) and the control group (1 cm). On day 14 showed wound healing in the treatment group, but the control group showed mean wound length on day 14 (0.15 cm). Test results on day 3 obtained p = 0.007, on day 7 obtained p= 0.010 and on day 14 obtained p-value = 0.017. P values on days 3, 7, and 14 showed significant differences with p<0.05. Conclusion: There are differences in wound healing and wound healing time at each concentration of turmeric extract gel. Turmeric extract gel concentration is the most effective healing wound cuts in female rats, namely the treatment group given EG 5%.
Development and Evaluation of Herbal Wound Healing Formulations
The aerial parts of Centella asiatica (L.) Urban and rhizomes Curcuma longa (L.) were collected, dried under shade and extracted with ethanol and water respectively. These extracts were dried. Aloe barbadensis concentrated gel powder was obtained as free sample. Each extract and Aloe gel was added with appropriate proportion in cream base and evaluated for wound healing activity against povidone iodine ointment. In excision wound model the complete wound healing was observed with cream formulation I and II treated rats was observed in 18 days where as povidone iodine ointment took 16 days. In incision wound model tensile strength of formulations I and II was found to be 366.08±2.32 and 351.35±3.29 gm respectively. Tensile strength of povidone iodine ointment was found 379.98±2.95 gm. Herbal formulation I and II took two days more for complete wound healing as compared to povidone iodine ointment. KEYWORDS Centella asiatica, Cream formulation, wound healing activity.
Wound Healing Activity of Traditional Herbal Formulation
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An indigenous herbal formulation containing, comphora officinarum (Kafoor), Shorea robusta (Raal), Beeswax (Apis mellifera), Acacia catechu (Katha safeed), Sesamum indicum (Til oil), and Azadirachta indica (Neem oil) was evaluated for wound healing activity in excision and incision wound models in albino rats. The activity was compared with that of the control and framycetin sulfate cream 1 % w/w as standard drug. The formulation showed a significantly higher contraction rate and shortened epithelization period in both the models. In excision model, the healing was 99% (p < 0.001) on 16 th day compared to 85 % and 75% of healing with framycetin sulfate cream and control, respectively. In incision wound model, there was significant increase in tensile strength (p < 0.001). Thus, it is concluded that the formulation has got potential wound healing activity for both the types of wounds; justifying its use in the traditional practice.
The aim of present study was to evaluate the wound healing activity of newly prepared polyherbal formulations ( i ,e Ointment, Gel and Liquid ) in Excision and Incision wound models in rats. The woundhealing activity was assessed by the rate of wound contraction, period of epithelialization and skin-breaking strength. In Excision wound model the polyherbal Ointment treated group exhibit 99.78±0.11% reduction in wound area on 20 th day when compared to standard Betadine Ointment and controls which was 98.55±0.44 % and 90.12±0.27% and faster rate of epithelization (16.55 ± 0.46). In the incision wound model, there was a significant increase in tensile strength (538.10± 1.52) was observed in ointment treated group. In all cases, there was a progressive decrease in wound area with time, indicating an efficacy of the formulations in healing the induced wounds. Our present study reveals that the new polyherbal formulations posses potent wound healing activity, which could be a good choice of remedy for wound healing.
Journal of Advanced Veterinary and Animal Research, 2017
Objective: This experiment was conducted to evaluate the therapeutic potency of marigold flower (Calendula officinalis) and turmeric (Curcuma longa) rhizome paste in wound healing. Materials and methods: Thirty six aseptic surgical wounds were tooled in six non-pregnant black Bengal goats dividing them in 3 groups. Month long information and follow-up examinations along with complications such as edema, wound dehiscence, suture abscess, exudation etc. were studied. Wound healing was assessed by observing some morphological characters as well as histopathological changes of the wounded area. Results: Results revealed that negligible elevation of suture line (1.17±0.11 mm) and significant (P<0.01) reduction of sutured area were observed in goats of turmeric group at day 3 (D3) and 7 (D7) respectively. Moreover, histologic appearance of wound of the similar group displayed splendid development of keratin layer and collagen fiber than the others from day 3 (D3). However, therapeutic efficacy of marigold flower paste was not up to the mark as turmeric in this experiment. Control group treated with povidone iodine, showed good results but took long time. Conclusion: This study could patronize veterinarians to consider the use of herbal plants specially turmeric as a great wound healer. It will also reduce toxicities created by haphazard use and applications of pharmaceutical products. The findings of this very experiment will not only prevent the skin degradation but also help to maintain the good health status of animals. Wound healing by marigold (Calendula officinalis) and turmeric (Curcuma longa) paste: A comparative approach December 2017 Vol 4 No 4, Pages 333-342. How to cite: Paul S, Rahman ANMA, Abdullah-Al-Mahmud M, Uzzal AR, Das ZC. Wound healing by marigold (Calendula officinalis) and turmeric (Curcuma longa) Paste: A comparative approach.
Study on Wound healing properties of polyherbal ointment
The Polyherbal ointment formulated with Acalypha indica(Linn) Gardenia jasminoides (Ellis) and Thespesia populnea(L) were tested for its wound healing property on experimentally induced Excision, Incision and Burn wounded rat models and also compared with reference ointment. Wistar strain of albino rats has been divided into 9 groups each comprising of 6 rats. Group -I, IV and VII served as wounded control, group -II, V and VIII were wounded and treated with polyherbal ointment topically, group -III, VI and IX were wounded and treated with reference drug. From the results obtained the polyherbal ointment treated group of excision and burn wound models showed significant increase in rate of wound contraction and in incision wound model a significant increase in breaking strength was observed. Levels of hydroxyproline, hexosamine, protein, DNA, RNA and activity of superoxide dismutase were markedly increased (p<0.05), with decreased level of lipid peroxide that depicts the healing of wound in the polyherbal ointment treated groups. Histological evaluation showed there were a marked infiltration, angiogenesis, fibrous tissue proliferation and collagen formation in greater levels in polyherbal ointment treated groups than control and reference groups. The results of the present study substantiate the traditional claims that the use of polyherbal ointment prepared from plants possesses significant wound healing promoting activity and provides scientific evidence to the ethno medicinal property of plants in the healing of wounds.
Screening of Wound Healing Activity of a Polyherbal Formulation
American Journal of Pharmacy and Pharmaceutical Sciences, 2014
The study aimed to prepare a polyherbal formulation containing Ficus bengalensis, Centella asiatica, Curcuma longa and to evaluate wound healing activity of polyherbal formulations. Both ointments formulated with hydro alcoholic and chloroform extract showed significant wound contraction during the first week and continued to promote decrease in wound size up to 16 th day. In excision wound model it was observed that the polyherbal formulated ointment of 2% chloroform extract showed a significant result when compared with polyherbal ointment formulation (hydro alcoholic). The wound healing activity of the prepared polyherbal ointment was found to be faster when applied on the experimental animals. The present study confirms the potential value of the polyherbal ointment as a wound healing product.