Clinical and Radiographic Characterization of Cerebrotendinous Xanthomatosis (CTX) (original) (raw)

2019, Japanese Society of Neurology

Introduction Mesenchymal stem cells (MSCs) are defined as a population of multipotent cells able to differentiate and produce any cell type needed in a repair process, such as osteoblasts, chondroblasts, neurons, epithelial cells, and cardiac cells (1,2). This cell type has become the focus of numerous studies worldwide for providing clinically promising perspectives for cell therapy and also for its immunomodulatory potential (3,4), although the mechanisms of immunosuppression on inflammatory response and the mechanisms of transplant rejection are not fully elucidated (5). Recent studies have described the use of allogeneic and autologous MSCs for the repair of various tissues (4,6). However, there is little research involving xenotransplantation in animals and most of them only evaluated the cellular interaction in vitro between MSCs and T lymphocytes. Because of the great therapeutic potential of MSCs, in addition to the persistent doubts about their immunosuppressive capacity in vivo, further studies are needed to investigate the real potential of xenogenic transplantation using these cells for tissue repair in animals. Therefore, this study evaluated clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived MSCs for the repair of radial bone defects created in rabbits. 2. Materials and methods This study was approved by the Institutional Animal Care Committee (protocol 97/2010). 2.1 Cellular culture A total of five male, 4-week old Wistar rats were euthanized using anesthetic overdose. The animals were immersed in alcohol 70° to ensure antisepsis for cell collection and were taken to the laminar flow cabinet. The femurs were disarticulated and removed aseptically. The distal epiphyses were cut and the medullary canal was flushed with Dulbecco's modified Eagle's medium (DMEM, Gibco, Grand Island, NY, USA) with low glucose, containing 10% fetal bovine serum (FBS) (Gibco), 50.0 mg L-1 gentamicin,