Biosynthesis, characterization of silver nano particles from Agaricus bisporus (Mushroom) and their antimicrobial activity against foodborne bacterial pathogens (original) (raw)
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Scientific reports, 2023
The emergence of antimicrobial resistance in foodborne bacterial pathogens has raised significant concerns in the food industry. This study explores the antimicrobial potential of biosynthesized silver nanoparticles (AgNPs) derived from Agaricus bisporus (Mushroom) against foodborne bacterial pathogens. The biosynthesized AgNPs were characterized using various techniques, including UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, highresolution scanning electron microscopy with energy dispersive X-ray spectroscopy, dynamic light scattering, and zeta potential analysis. The antibacterial activity of the AgNPs was tested against a panel of foodborne bacterial strains, and their cytotoxicity was evaluated on normal human skin fibroblasts. Among the tested strains, Pseudomonas aeruginosa ATCC 27853 showed the highest sensitivity with an inhibition zone diameter (IZD) of 48 mm, while Klebsiella quasipneumoniae ATTC 700603 and Bacillus cereus ATCC 11778 displayed the highest resistance with IZDs of 20 mm. The silver cations released by AgNPs demonstrated strong bactericidal effects against both Gram-positive (G + ve) and Gram-negative (G − ve) bacteria, as evidenced by the minimum inhibitory concentration/ minimum bactericidal concentration (MBC/MIC) ratio. Moreover, cytotoxicity testing on normal human skin fibroblasts (HSF) indicated that AgNPs derived from the mushroom extract were safe, with a cell viability of 98.2%. Therefore, AgNPs hold promise as an alternative means to inhibit biofilm formation in the food industry sector. The food sector faces critical problems due to bacterial resistance towards antibiotics, chemical cleansers and disinfectants. Foodborne pathogens affect the healthcare settings, as they can cause foodborne illnesses in patients
Molecules (Basel, Switzerland), 2018
The present study demonstrates an economical and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using the wild mushroom . The synthesis of AgNPs was confirmed and the products characterized by UV-visible spectroscopy, dynamic light scattering spectroscopy and X-ray diffraction analysis. Furthermore, Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to identify the viable biomolecules involved in the capping and active stabilization of AgNPs. Moreover, the average sizes and morphologies of AgNPs were analyzed by field emission scanning electron microscopy (FE-SEM). The potential impacts of AgNPs on food safety and control were evaluated by the antimicrobial activity of the synthesized AgNPs against common food-borne bacteria, namely, , , , and . The results of this study revealed that the synthesized AgNPs can be used to control the growth of food-borne pathogens and have potential application in the food packaging industry. Moreover, th...
Journal of Chemistry, Environmental Sciences and its Applications, 2020
The present study was aimed to synthesize silver nanoparticles (AgNPs)using the extract of oyster mushroom Pleurotuscitrinopileatus and its use in antibacterial testing. This green synthesis approach of silver NPs is very fast, simple, environmentally friendly and economical. The initial confirmation of silver NPs synthesis was observed with the alteration of the colour of the solution from colourless to wine red. The prepared nano-material was further characterized by UV-Visible spectrophotometer, and transmission electron microscopy (TEM). The TEM revealed particle size of synthesized AgNPsbetween the range of 11-13 nm. The bactericidal efficacy of silver NPstested against Escherichia coli confirmed the lowest 50 µg/L concentration of silver NPs bactericidal. Therefore based the observations of the study silver NPsat the level of its 50 µg/L can be used for the purposes of potential water disinfection, killing of bacteria, disinfection of medical equipments, wound washings, preser...
Due to the growing demand to improve an environmentally friendly, nonhazardous and cost-effective technology as a biocide to control the drug-resistant microorganism, thus in the present study; food-origin Escherichia coli, Candida zeylanoides and C. krusei were used for the biosynthesis of silver nanoparticles. The biosynthesis of silver nanoparticles was monitored upon the colour change of the reaction mixture by ultraviolet-visible spectroscopy. Furthermore, the product was explored by Atomic Force Microscopy; the results revealed the formation of silver nanoparticles in the reaction mixture of the used microorganisms. The biosynthesized silver nanoparticles from E. coli, C. zeylanoides and C. krusei showed a maximum absorption at 423 nm, 415 nm and 426 nm respectively at range between 300 to 800nm wavelength and the size was 64.93 nm, 102.86 nm and 95.37 nm respectively. The biosynthesized silver nanoparticles were tested for their antimicrobial activity against various pathogens Pseudomonas flourescence, Klebsiella pneumonia, E.coli, Candida guilliermondii, and C. albicans; the results showed that the silver nanoparticles that biosynthesized in the current research exhibited an effective antimicrobial activity.
researchgate.net, 2011
In this paper we report an eco-friendly route for the synthesis of sliver nanoparticles using Agaricus bisporus (white button mushroom) extract. The synthesized silver nanoparticles were confirmed and characterized by UV-Visible spectrum of the aqueous solution containing silver ions showed a peak at 420 nm corresponding to the surface plasmon absorbance of silver nanoparticles. Transmission Electron Microscopy (TEM) micrographs showed the size of silver nanoparticles and were measured in the range of 8-50nm, Fourier Transform Infrared Spectroscopy (FTIR) spectrum detection of analysis showed peaks between 500-4000 cm-1 confirmed the presence of proteins, carbonyl groups, esters and carboxylic acids for the synthesis and stabilization of silver nanoparticles .X-ray diffraction (XRD) spectrum of the silver nanoparticles exhibited 2θ values corresponding to the silver nanocrystal. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against pathogens and nonpathogenic bacterial strains vis, Escherichia coli, Staphylococcus sps, pseudomonas sps, and Bacillus sps.
Biomolecules
The biosynthesis of silver nanoparticles (Ag NPs) could play a significant role in the development of commercial antimicrobials. Herein, the biosynthesis of Ag NPs was studied using the edible mushroom Pleurotus floridanus, and following its formation, spectrophotometry was used to detect the best mushroom content, pH, temperature, and silver concentration. After that, the morphology was described via transmission electron microscopy (TEM), and nanoscale-size particles were found ranging from 11 to 13 nm. The best conditions of Ag content and pH were found at 1.0 mM and 11.0, respectively. In addition, the best mushroom extract concentration was found at 30 g/L. According to XRD analysis, the crystal structure of the formed amorphous Ag NPs is cubic with a space group of fm-3m and a space group number of 225. After that, the function groups at the surface of the prepared Ag NPs were studied via FTIR analysis, which indicated the presence of C=O, C-H, and O-H groups. These groups cou...
The development of appropriate processes for the synthesis of nanoparticles is an important aspect of nanotechnology. In this study, fungus mediated synthesis of silver nanoparticles using Microporus Xanthopus has been done. The dried macro fungi were powdered and boiled for 20 minutes to get the extracts. The extracts were treated with silver nitrate and kept in the dark conditions for 48 hrs, for the appearance of colour change. The Plasmon peak was observed at 425 nm. These nanoparticles were examined for the stability for a period of 2 months and were further characterized by SEM and FTIR. SEM analysis revealed the spherical nature of silver nanoparticles and size distributed in the range of 40 nm. However further studies regarding XRD, SEM, EDX, are confirmed to strengthen the existing findings. The synthesized silver nanoparticles have been investigated against the pathogenic bacteria Bacillus subtilis , Staphylococcus aureus, Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae and shigella sp for their antibacterial activity and minimum inhibitory concentration.
Preparation of Nano-Silver Particles by Chemical Method for Anti-Bacteria’s Application
TJPRC, 2013
In this search , Nano-silver particles were prepared by chemical method(reduction method), the homogenous nano silver particles with size average (58.82 nm) were obtained from this method, different molar concentrations of reactive materials were used, the optimum molar concentration give this size average, Stable Ag Nano particles were prepared, their shapes and size distribution characterized by particle characterizer and scanning electron microscopic (SEM), and force electron microscopic (AFM) are used, the (DLS) Zetazaser tool was used to calculate of Zeta Potential and (PDI) of resulting nanoparticles , these results were showed by figure (3a,b), AFM and SEM pictures were showed in figure (4,5),The antimicrobial activity of Ag nanoparticles was investigated against yeast , the bio bacterial activity was showed these nanoparticles were very active to (E colie) bacteria.