Oxidative stress in autoimmune rheumatic diseases (original) (raw)

Role of oxidant stress in rheumatoid arthritis

Oxygen derived free radicals have been implicated in the causation of Rheumatoid arthritis (RA). In this study, evidence of free radical injury and oxidative stress in patients with RA is compared with healthy subjects by estimating superoxide dismutase (SOD) and catalase, which are anti-oxidant enzymes in RBCs, Glucose 6 Phosphate Dehydrogenase (G 6 PD) in RBCs and serum Malon-di-aldehyde (MDA) levels. Serum MDA levels in RA could be used as a biochemical marker of disease activity and for monitoring the response to treatment. There was no definite correlation between enzyme levels of SOD, catalase and G 6 PD and disease activity.

Oxidative stress in patients with rheumatoid arthritis

Revista de investigación clínica; organo del Hospital de Enfermedades de la Nutrición

Rheumatoid arthritis is an autoimmune disease of unknown etiology, characterized by articular inflammation. Oxidative damage induced by reactive oxygen species has been related to the pathophysiology of rheumatoid arthritis in several studies, although results have been inconsistent and contradictory. To determine oxidative stress markers in patients with rheumatoid arthritis. Descriptive cross-sectional study in rheumatoid arthritis patients and healthy controls. In peripheral blood samples from all study subjects, lipid peroxide (thiobarbituric acid reactive substances) and protein carbonyl levels were quantified as oxidative damage markers; superoxide dismutase and glutathione peroxidase activities, glutathione concentration, and the reduced glutathione/oxidized glutathione ratio were analyzed as antioxidant defense indicators. Mann-Whitney U tests were run. Statistical significance (a) was 0.05%. We included 29 rheumatoid arthritis patients, 10 with active disease, and 41 health...

-The role of oxidative strees in inflammation patients with rheumatoid

MPACT Journals, 2015

The aim of this study isto determine the level of blood markers of cellular oxidative stress and then to establish the oxidative profile in 145 patients with rheumatoid arthritis (RA).The samples of the study include patients with RA. They were achieved four or more of the criteria of the 2010 American College of Rheumatology, the sample of the study was conducted to fifty persons apparently healthy volunteered to perform the test of this study. We determined the plasmatic levels of malondialdehyde, compared with the inflammatory parameters. Results: in comparison to controls, patients with juvenile rheumatoid arthritis presented high concentrations of lipid per oxidation products (determined by plasmatic levels of malondialdehyde). concluded our results which indicate the presence of molecular damage determined by oxygen free radicals in patients with rheumatoid arthritis. KEYWORDS : Free Radicals, Inflammation, Malondialdehyde (MDA), Rheumatoid Arthritis, ROS

Oxidative status in rheumatoid arthritis

Clinical Rheumatology, 2007

The insufficiency of antioxidant defense systems and the acceleration of the oxidative reactions can be results of the pro-oxidant/antioxidant imbalance in rheumatoid arthritis (RA). The aim of our study was to investigate the changes in oxidant status by measuring two different parameters; one was the level of malondialdehyde (MDA) as an index of lipid peroxidation and the other was total oxidative status; we could then compare our results with the antioxidant status, superoxide dismutase (SOD) enyzme activities. All were assessed in 22 patients with active RA and 18 age-and gender-matched control subjects. While serum MDA levels were significantly increased in patients with RA compared to the control group (p<0.03), the total oxidative status levels were decreased in patients with RA compared to the control group (p<0.008), and serum SOD activities did not show any statistical difference between the two groups. In conclusion, the increased MDA levels in our study may be important as a marker but are not sufficient to conclude that there was an increase in oxidative stress in RA patients because supporting results were not obtained from SOD and oxidative status measurements. These results give further support to the concept of oxygen free radicals playing a role in the pathogenesis of chronic inflammatory disorders, but we also consider that there is a more complex relationship than has been assumed. We think that further studies are needed to clarify these conflicting results.

Reactive oxygen species in inflammation

2013

ISBN 978-951-29-5352-3 (PRINT) ISBN 978-951-29-5353-0 (PDF) ISSN 0355-9483 Painosalama -Turku, Finland, 2013 "The whole of science is nothing more than a refinement of everyday thinking." Albert Einstein "Desperation is a necessary ingredient to learning anything, or creating anything.

Investigation of protein oxidation and lipid peroxidation in patients with rheumatoid arthritis

Cell Biochemistry and Function, 2006

We aimed to determine the importance of neutrophil activation and the source of oxidative stress in the pathogenesis of rheumatoid arthritis (RA) by quantification of advanced oxidation protein products (AOPP) and total thiol levels as markers of oxidative protein damage, malondialdehyde (MDA) levels as a marker of lipid peroxidation and myeloperoxidase (MPO) activity as a marker of neutrophil activation in patients with RA. Fifty-seven rheumatoid arthritis patients were included in the study and sub-grouped according to disease activity (active, n = 31; inactive, n = 26) and compared with healthy controls (n = 25). Serum MPO activity, AOPP, MDA, and thiol levels were measured by an enzymic spectrophotometric method. Serum MPO activity (p < 0.001), AOPP (p < 0.001), MDA (p < 0.001) and levels of thiol (p < 0.002), were higher in the patient group than the controls. Active and inactive RA groups were compared with the control group and there were significant differences between each parameter. MPO activity, AOPP, MDA and thiol levels were significantly higher in both active and inactive RA patients than the controls. On the other hand, when a comparison was made between active and the inactive stage, a statistically significant difference was present only in MDA (p < 0.05) and AOPP levels (p < 0.05). There was also a significant positive correlation between all parameters. These data strongly suggest that neutrophils, which constitute the most important source of chlorinated oxidants due to their high MPO content, may be involved in serum AOPP formation and therefore the production of a novel class of pro-inflammatory mediators of oxidative stress in RA patients and that protein oxidation could play an important role in the pathogenesis of RA as does lipid peroxidation. Copyright © 2005 John Wiley & Sons, Ltd.

Oxidative stress parameters in different systemic rheumatic diseases

Journal of Pharmacy and Pharmacology, 2006

The involvement of oxidative stress in the pathogenesis of rheumatic disorders, such as systemic sclerosis (SSc) and chronic polyarthritides, has been suggested yet not thoroughly verified experimentally. We analysed 4 plasmatic parameters of oxidative stress in patients with SSc (n = 17), psoriatic arthritis (PsA) (n = 10) and rheumatoid arthritis (RA) (n = 9) compared with healthy subjects (n = 22). The biomarkers were: total antioxidant capacity (TAC) measured by ferric reducing antioxidant power (FRAP) method, hydroperoxides determined by ferrous ion oxidation in presence of xylenol orange (FOX) method and sulfhydryl and carbonyl groups assessed by spectrophotometric assays. The results showed significantly increased hydroperoxides in SSc, PsA and RA (3.97 ± 2.25, 4.87 ± 2.18 and 5.13 ± 2.36 μmol L−1, respectively) compared with the control group, (2.31 ± 1.40 μmol L−1; P < 0.05). Sulfhydryls were significantly lower in SSc (0.466 ± 0.081 mmol L −1), PsA (0.477 ± 0.059 mmol L−1) and RA (0.439 ± 0.065 mmol L−1) compared with the control group) (0.547 ± 0.066 mmolL−1; P < 0.05). TAC in all three diseases showed no difference in comparison with controls. Carbonyls were significantly higher in RA than in the control group (32.1 ± 42 vs 2.21 ± 1.0 nmol (mg protein)−1; P < 0.05). The obtained data indicate augmented free radical-mediated injury in these rheumatic diseases and suggest a role for the use of antioxidants in mediated prevention and treatment of these pathologies.

Oxidative stress and antioxidant status in patients with rheumatoid arthritis

2013

Rheumatoid Arthritis (RA) is a multifactorial autoimmune disease affecting around 1% of the world’s population leading to autoimmune arthritis. Though the exact etiology of RA remains unknown, various environmental and biological triggers have been suspected. The most important factor which is implicated in the pathogenesis of RA is oxidative stress. However the exact relationship between antioxidants and lipid peroxidation is not yet known in patients of RA. Hence the case control study was conducted in 50 patients of rheumatoid arthritis (Group II) diagnosed by criteria’s recommended by American Rheumatology Association were compared with age and sex matched 50 normal healthy controls (GroupI). Malondialdehyde (MDA) a marker of oxidative stress, antioxidant enzymes Glutathione peroxidase (GPx) and Superoxide dismutase (SOD) were measured by enzymatic spectrophotometric method. MDA levels was significantly increased (p< 0.01) in RA patients as compared to controls. The concentra...