HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITY OF ALPINIA MALACCENSIS ROSCOE RHIZOME (original) (raw)

2015, International Journal of Pharmacy and Pharmaceutical Sciences

Objective: The present study aims to evaluate hepatoprotective activity, antioxidant activity and total phenolic content of methanolic extract from rhizomes of A. malaccensis. Methods: The hepatoprotective activity in mice was studied by inducing oral dose at 250 mg/kg and 500 mg/kg b. wt., antioxidant activity was evaluated by different method viz; the superoxide anion scavenging activity, hydroxyl radical scavenging activity, nitric oxide scavenging activity, DPPH radical scavenging activity and total phenolic content. Results: The mice were significantly re established from hepatotoxicity evident by analyzing the factors such as triglycerides, ALT, AST, glucose, cholesterol, total protein, bilirubin, albumin and urea levels. Further more histopathological results revealed the significant improvement of liver. Methanolic extracts of rhizomes of A. malaccensis showed abilities to scavenge hydroxyl, superoxide free radicals, nitric oxide, DPPH with IC50 values of 50.42, 71.19, 96.38, 61.46 µg/ml and reducing power ability with the RP50 value of 183.54 µg/ml. The methanolic extract was found to have a total phenolic content of 48.18 µg/ml. Conclusion: Our study reveals the therapeutic effect of A. malaccensis on treated mice as well as the strong antioxidant potential with its total phenolic content. A. malaccensis so may be used as herbal medicine to replace synthetic one.