Evaluation of Genetic Diversity among Soybean (Glycine max) Genotypes, Using ISJ and RAPD Molecular Markers (original) (raw)
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Molecular Diversity Analysis of Soybean Genotypes Using Molecular Markers
Excellent Publishers, 2017
Soybean is legume crop having high protein (40%) and oil (20%) content. It has highest share in production among all oilseeds of the world. In present study 10 soybean genotypes were characterized using 14 RAPD primers. Total 103 amplicons were obtained out of which 75 were polymorphic and 28 were monomorphic. The percent polymorphism obtained was 72.81%. Highest average similarity coefficient was exhibited by the genotype MAUS-32 (0.675) and lowest similarity coefficient exhibited by MAUS-2 (0.584). The dendrogram of these genotypes grouped into two main clusters. These two clusters again divided into 6 sub-clusters having distinct morphological and physiological characteristics.
Journal of microbiology, biotechnology and food sciences
Soybean (Glycine max L.) is an important crop plant which contains a high amount of oil and protein. The main goal of the work was to use 13 RAPD (Random Amplified Polymorphic DNA) markers to study genetic polymorphism in a set of 28 soybean genotypes and to construct a dendrogram from the obtained results, based on which we will recommend genotypes for further breeding. In total, using 13 RAPD markers, we determined 108 fragments in a set of 28 soybean genotypes with an average number of 8.31 fragments per genotype. The number of fragments varied from 6 (OPB-08, OPE-07) to 12 (SIGMA-D-01). Of the total number of 108 fragments, 70 were polymorphic with an average number of 5.38 polymorphic fragments per genotype. The number of polymorphic fragments varied from 3 (OPE-07, OPF-14) to 9 (SIGMA-D-01). The average percentage of fragment polymorphism was 63.68% and ranged from 42.86% (OPF-14) to 83.33% (OPB-08). Diversity index (DI) values ranged from 0.710 (OPA-03) to 0.846 (OPD-08) with...
Genetic Variability and Diversity Studies in Soybean [Glycine max (L.) Merrill] using RAPD Marker
Soybean (Glycine max) is an important vegetable oilseed crop. It is considered to be a cash crop. It is a major source of edible vegetable oils and proteins which contains about 40% protein and 20% oil. The genetic diversity was estimated among 7 varieties of Soybean using 22 RAPD primers. The RAPD marker is useful tool for assessing genetic variation and resolving cultivars identities. Among the 22 primers, 11 primers showed polymorphism.
Molecular Biology Reports, 2021
Background The genetic base of soybean cultivars in India has been reported to be extremely narrow, due to repeated use of few selected and elite genotypes as parents in the breeding programmes. This ultimately led to the reduction of genetic variability among existing soybean cultivars and stagnation in crop yield. Thus in order to enhance production and productivity of soybean, broadening of genetic base and exploring untapped valuable genetic diversity has become quite indispensable. This could be successfully accomplished through molecular characterization of soybean genotypes using various DNA based markers. Hence, an attempt was made to study the molecular divergence and relatedness among 29 genotypes of soybean using SSR markers. Methods and results A total of 35 SSR primers were deployed to study the genetic divergence among 29 genotypes of soybean. Among them, 14 primer pairs were found to be polymorphic producing a total of 34 polymorphic alleles; and the allele number for...
International Journal of Chemical Studies, 2019
An experiment was conducted to assess the genetic diversity in soybean using Simple Sequence Repeat (SSR) diversity of 24 elite soybean genotypes were selected for high yield potential. The four primers showed reliable polymorphism produced a total of 92 bands of which 68 (75%) were polymorphic. Genetic similarity estimates based on simple matching coefficients revealed more genetic diversity among all the 24 elite lines, ranging from 0.143 to 1 indicating the distinctness of these genotypes under study. The dendrogram constructed using the UPGMA method separated these genotypes in two main groups each having two sub groups. The study also demonstrates high reliability, ease of applicability and importance of SSR markers in evaluating genetic variation among the genotypes of soybean.
Analysis of genetic diversity of soybean germplasm from five different origins using RAPD markers
Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 2016
The pattern of genetic diversity among 92 genotypes of soybean from 5 different origins/ sources (Pakistan, the USA, Asian Vegetable Research Development Centre (AVRDC), Japan and North Korea) was analyzed using randomly amplified polymorphic DNA markers. Out of 20 random primers 6 tested, 10 were polymorphic among genotypes and they yielded 107 markers, with an average of 10.7 markers per primer. The proportion of polymorphic bands within genotypes ranged from 0.47 to 0.71 with an average of 0.59. Pakistani and US genotypes exhibited the highest number of polymorphic bands (95%), while North Korean genotypes revealed the lowest (60%). The mean band frequency of the primers among genotypes was 0.57 with a range of 0.08-0.99. The Shannon's index and Nei's genetic diversity index revealed that primer OPF-06 showed maximum genetic diversity among the genotypes. Dendrogram constructed using Unweighted Pair Group Mean Average (UPGMA) method divided the genotypes into 5 main groups consisting of 13 clusters. The results of cluster analysis indicated that the genetic diversity between Pakistani and US or AVRDC genotypes is much larger than that between Pakistani and North Korean or Japanese genotypes. The Pakistani genotypes had distinct bands from plant introductions. Therefore, the Pakistani genotypes may be useful to soybean breeders.
Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers
Australian Journal of Crop Science
In this study, we aimed to investigate the genetic diversity and polymorphism among 30 soybean genotypes maintained by the ARC using simple sequence repeat (SSR) markers. Soybean genotypes were characterized using 20 SSR primers. DNA was extracted using the standard cetyl trimethylammonium bromide method and amplified using PCR. Allele size was determined via comparison with a 100 base pair (bp) DNA ladder. Molecular data were analyzed, and a dendrogram and matrix were generated using GGT 2.0 software. A total of 216 alleles with an average of 10.8 alleles per locus were detected. The allele sizes ranged between 2 and 33 bp with an average of 18.7 bp. The polymorphic information content among genotypes varied from 0.85 (Satt001) to 0.75 (Satt43) with an average of 0.716, and heterozygosity ranged from 0.87 to 0.78 with an average of 0.7485. The most diverse genotypes were B 66 S 31, 69S 7, and R5-4-2 M, which indicated the efficiency of the SSR markers for the detection of genetic d...
sing protein and DNA fingerprinting to study cultivars may provide information about genetic relationships between them that may be useful to plant breeders. The genetic diversity between eleven cultivars of soy bean was examined based on polymorphism using SDS-PAGE of seed protein and RAPD analysis. The protein banding pattern showed 59.1% polymorphism between the studied genotypes. Five primers generated a total of 47 RAPD markers of which 29 bands were polymorphic (61.7 %). The genetic relationships between the eleven cultivars based on variations in protein bands and RAPD fragments have been estimated by the NTSYS-pc software using UPGMA and Neighbor Joining (NJ) tree building methods. The two trees separated the studied cultivars into two main groups. The first group includes the two cultivars Dr101 and Clark and the second group is clustered in two subgroups; one comprising Giza 35 and Giza 111 and the other includes the remaining cultivars. In the NJ tree the two cultivars Giza 22 and Giza 82 were assigned to the first subgroup but UPGMA put them in the second subgroup with other varieties. Each of the following cultivars; Giza 21,Crawford and Holladay was clearly distinct in the second subgroup. The two cultivars Giza 83 and Toano are closely related in UPGMA and NJ trees.
Agricultural Research, 2019
Genetic diversity among 45 genotypes of soybean (Glycine max (L.) Merr.) was assessed based on qualitative traits and 36 microsatellite markers. Forty-five genotypes were characterized for eleven morphological traits: leaf shape, leaf intensity of green colour, leaf size of lateral leaflet, plant growth habit, variation in hilum colour, cotyledon/flower colour, hairiness, hair colour, pod colour, testa colour and seed shape. Principal component analysis revealed that genotypes, namely JS-95-60, JS-20-103, JS20-69, JS-20-114, JS-20-49 and JS-335, were diverged from each other, and the range of polymorphic information content for microsatellite markers was 0.018-0.580. With 27 polymorphic SSR markers, a total of 71 alleles were amplified with an average of 1.97 alleles per locus. Eleven alleles were found to be unique to 45 genotypes. Soybean genotypes distributed in two clusters revealed diverse genetic background. Overall, the present study paves the way for better characterization of soybean genotypes and confirms India as one of the important centres of soybean domestication containing valuable genetically important assets for soybean improvement.