Correction: A modified aeroponic system for growing small-seeded legumes and other plants to study root systems (original) (raw)
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A modified aeroponic system for growing small-seeded legumes and other plants to study root systems
Plant Methods
Background Various growth systems are available for studying plant root growth and plant–microbe interactions including hydroponics and aeroponics. Although some of these systems work well with Arabidopsis thaliana and smaller cereal model plants, they may not scale up as well for use with hundreds of plants at a time from a larger plant species. The aim of this study is to present step-by-step instructions for fabricating an aeroponic system, also called a “caisson,” that has been in use in several legume research labs studying the development of symbiotic nitrogen fixing nodules, but for which detailed directions are not currently available. The aeroponic system is reusable and is adaptable for many other types of investigations besides root nodulation. Results An aeroponic system that is affordable and reusable was adapted from a design invented by French engineer René Odorico. It consists of two main components: a modified trash can with a lid of holes and a commercially availab...
Architecture of the Root System of Potato Cultivars Grown in Aeroponics
Electronic Journal of Polish Agricultural Universities, 2018
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The study of Lycopersicum esculentum rooting in aero-hydroponic system
Analele Universității din Oradea, Fascicula: Protecția Mediului, 2015
The base principle in an aero-hydroponic system is to grow the plants suspended into a closed or half-closed environment, by pulverizing the suspended roots with a mixture of water and water-soluble nutrients, while the aerial part develops on top. The study was performed between 9 th April-7 th May 2015 in the USAMV Cluj-Napoca's Greenhouse. For the experiment rooted tomato seeLSDings were used and three treatment variants were studied: V1-rooted tomato seeLSDings; V2-tomato seeLSDings with chopped roots and treated with gel; V3-tomato seeLSDings with chopped roots and not treated. After one week from the experiment's start, the tomato plants with chopped roots have formed new roots with lengths of 3.83 cm (V3) and 2.25 cm (V2). The root's length is greater for the chopped roots treated with gel compared to the ones not treated, data being statistically significant in favour of the treated variant.
. Aeroponics System of Cultivation in Horticultural Crops
Population of earth is expected to rise by 3 billion people. It is estimated that approximately 109 hectares of additional traditional land will be needed to feed them. Only 80% of the Earth's arable land is suitable for farming now. A greater quantity of hectares with optimum inputs is needed every day to feed the rising population. This chain of high priority problems requires an improvement in the management of the use of resources so that human consumption has the priority in its use. To solve the problems mentioned, new farming methods have been searched, one of them being aeroponics. With this technique, the plants are held by certain structures that are maintained in a way that the roots are sustained up in the air. Aeroponic literally means "growing in air." An aeroponic system is medium-less in that the roots of the plant are free hanging inside an open root-zone atmosphere. Aeroponics structure supplies optimum levels of water, nutrients and air to the growing chamber. Aeroponics is the process of growing plants in an air or mist environment without use of soil or an aggregate media. The word aeroponic is derived from the Latin word 'aero' (air) and 'ponic' means labour (work). This is an alternative method of soil-less culture in growth-controlled environments. The aeroponic culture technique is an optional device of soil-less culture in growth-controlled environments such as greenhouses. This method consists of enclosing the root system in a dark chamber and supplying a nutrient solution of mist device. This was widely used in horticultural species including potato, tomato, lettuce, cucumber and ornamental plants such as chrysanthemum. Aeroponic systems for seed production have been established following increased demand for more efficient high quality seed production
Evaluation of aeroponics for clonal propagation of Caralluma edulis, Leptadenia reticulata and Tylophora indica – three threatened medicinal Asclepiads, 2014
Abstract The present study explores the potential of aeroponic system for clonal propagation of Caralluma edulis (Paimpa) a rare, threatened and endemic edible species, Leptadenia reticulata (Jeewanti), a threatened liana used as promoter of health and Tylophora indica (Burm.f.) Merill, a valuable medicinal climber. Experiments were conducted to asses the effect of exogenous auxin (naphthalene acetic acid, indole-3-butyric acid,indole-3-acetic acid) and auxin concentrations (0.0, 0.5, 1, 2, 3, 4 or 5gl−1) on various root morphological traits of cuttings in the aeroponic chamber. Amongst all the auxins tested, significant effects on the length, number and percentage of rooting was observed in IBA treated nodal cuttings.Centper centofthe stemcuttingsofC.edulisrooted if pre-treated with 2.0 gl−1 of IBA for 5 min while 97.7 % of thestemcuttingsofL.reticulata and93.33%ofstemcuttings of Tylophora indica rooted with pre-treatment of 3.0 gl−1 of IBA for 5 min. Presence of at least two leaves on the nodal cuttings of L. reticulata and T. indica was found to be a prerequisite for root induction. In all the species, the number ofadventitiousrootspercuttingandthepercentageofcuttings rooted aeroponically were significantly higher than the soil grown stem cuttings. Shoot growth measured in terms of shoot length was significantly higher in cuttings rooted aeroponically as compared to the cuttings rooted under soil conditions. All the plants sprouted and rooted aeroponically survived on transfer to soil. This is the first report of clonal propagation in an aeroponic system for these plants. This study suggests aeroponics as an economic method for rapid root induction and clonal propagation of these three endangeredandmedicinallyimportantplantswhichrequirefocused efforts on conservation and sustainable utilization.
Plant Methods
Background Root and tuber crops are becoming more important for their high source of carbohydrates, next to cereals. Despite their commercial impact, there are significant knowledge gaps about the environmental and inherent regulation of storage root (SR) differentiation, due in part to the innate problems of studying storage roots and the lack of a suitable model system for monitoring storage root growth. The research presented here aimed to develop a reliable, low-cost effective system that enables the study of the factors influencing cassava storage root initiation and development. Results We explored simple, low-cost systems for the study of storage root biology. An aeroponics system described here is ideal for real-time monitoring of storage root development (SRD), and this was further validated using hormone studies. Our aeroponics-based auxin studies revealed that storage root initiation and development are adaptive responses, which are significantly enhanced by the exogenous...
Horticulturae, 2018
Aeroponics is a relatively new soilless culture technology which may produce food in space-limited cities or on non-arable land with high water-use efficiency. The shoot and root growth, root characteristics, and mineral content of two lettuce cultivars were measured in aeroponics, and compared with hydroponics and substrate culture. The results showed that aeroponics remarkably improved root growth with a significantly greater root biomass, root/shoot ratio, and greater total root length, root area, and root volume. However, the greater root growth did not lead to greater shoot growth compared with hydroponics, due to the limited availability of nutrients and water. It was concluded that aeroponics systems may be better for high value true root crop production. Further research is necessary to determine the suitable pressure, droplet size, and misting interval in order to improve the continuous availability of nutrients and water in aeroponics, if it is to be used to grow crops suc...
2008 Roumet et al Plant and Soil
This study examines whether root traits differed between three major plant families (Asteraceae, Fabaceae and Poaceae) and whether they are related to root respiration and exudation. Nine traits related to biomass allocation, root topology, morphology, chemical composition and mycorrhizal colonisation were examined for nine C 3 herbaceous species grown in controlled conditions. Poaceae differed from Fabaceae for the whole set of root traits examined except mycorrhizal colonisation, while Asteraceae showed intermediate characteristics. As compared to Fabaceae, Poaceae allocated more biomass to roots; showed a more sparsely branched root system with a small average root diameter, a high root dry matter content and a low nitrogen concentration. Root respiration was weakly related to root mass ratio and root dry matter content; no significant relationship was found between root functions and root architecture or morphology. This study shows that plant classification based on taxonomic affiliation reflects differences in root system traits and functions. Whole root system traits do not allow strong predictions of root respiration and exudation, perhaps because these processes are more linked to fine root than to whole root system traits.
An aeroponic culture system for the study of root herbivory on Arabidopsis thaliana
Plant Methods, 2011
Background: Plant defense against herbivory has been studied primarily in aerial tissues. However, complex defense mechanisms have evolved in all parts of the plant to combat herbivore attack and these mechanisms are likely to differ in the aerial and subterranean environment. Research investigating defense responses belowground has been hindered by experimental difficulties associated with the accessibility and quality of root tissue and the lack of bioassays using model plants with altered defense profiles. Results: We have developed an aeroponic culture system based on a calcined clay substrate that allows insect herbivores to feed on plant roots while providing easy recovery of the root tissue. The culture method was validated by a root-herbivore system developed for Arabidopsis thaliana and the herbivore Bradysia spp. (fungus gnat). Arabidopsis root mass obtained from aeroponically grown plants was comparable to that from other culture systems, and the plants were morphologically normal. Bradysia larvae caused considerable root damage resulting in reduced root biomass and water absorption. After feeding on the aeroponically grown root tissue, the larvae pupated and emerged as adults. Root damage of mature plants cultivated in aeroponic substrate was compared to that of Arabidopsis seedlings grown in potting mix. Seedlings were notably more susceptible to Bradysia feeding than mature plants and showed decreased overall growth and survival rates.