Avian Infectious Laryngotrachietis Infection Virus in Some Domestic and Captured Wild Birds in Yobe State, Nigeria (original) (raw)
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Sero-Surveillance of Infectious Laryngotracheitis in Layer Birds in Bangladesh
Microbes and Health, 2013
Infectious Laryngotracheitis (ILT) is an economically important respiratory disease primarily of chicken caused by Infectious Laryngotracheitis Virus (ILTV) belonging to the family Herpesviridae. After infecting its host, the virus induces immune response; antibodies are produced, which can be easily detected by several immunological or serological methods like Enzyme-Linked Immunosorbent Assay (ELISA). In the present study, the research work was conducted to reveal the prevalence of antibodies (IgG) against ILTV in layer birds in Bangladesh during the period of July 2010 to April 2011. A total of 324 blood samples were collected randomly from 30 layer farms located in 15 different districts in the country, and subsequently sera were prepared. Aiming to detect the antibody against ILTV, the sera samples were subjected to indirect ELISA using a commercial ILTV Antibody Test kit. Out of 324 sera samples examined, a total of 299 (92.28%) were positive. The results indicate that layers ...
Serologic and Molecular Survey of Avian Infectious Laryngotracheitis in Ecuador
ECUADOR ES CALIDAD - Revista Científica Ecuatoriana
The serologic survey and the confirmatory molecular diagnostic suggested evidence of a fairly presence of ILT infection in the main poultry farms of Ecuador. The current work highlights the need to apply a control program of ILT based on vaccination and standard biosecurity measures in the poultry farms of Ecuador.
Natural infection of turkeys by infectious laryngotracheitis virus
Veterinary Microbiology, 2008
The infectious laryngotracheitis virus (ILTV) is an important respiratory pathogen of chickens that also infects pheasants and peafowl. Epidemiologically non-related commercial turkey flocks with clinical signs such as tracheitis, swollen sinuses, conjunctivitis and expectoration of bloody mucus were examined for the presence of the virus. Laboratory ILTV detection was performed by virus isolation in embryonated eggs and cell cultures, PCR and sequencing of amplification products, histopathology, indirect immunofluorescence and electron microscopy. One ILTV turkey isolate was also experimentally inoculated into susceptible chickens and turkeys, reproducing a mild respiratory disease. This is the first description of natural infections with ILTV in turkeys. #
The Scientific World Journal
Infectious laryngotracheitis (ILT) is a disease of high economic consequence to the poultry sector. Gallid herpesvirus 1 (GaHV-1), a.k.a infectious laryngotracheitis virus (ILTV), under the genus Iltovirus, and the family Herpesviridae, is the agent responsible for the disease. Despite the clinical signs on the field suggestive of ILT, it has long been considered nonexistent and a disease of no concern in Ethiopia. A cross-sectional study was conducted from November 2020 to June 2021 in three selected zones of the Amhara region (Central Gondar, South Gondar, and West Gojjam zones), Ethiopia, with the objective of estimating the seroprevalence of ILTV in chickens and identifying and quantifying associated risk factors. A total of 768 serum samples were collected using multistage cluster sampling and assayed for anti-ILTV antibodies using indirect ELISA. A questionnaire survey was used to identify the potential risk factors. Of the 768 samples, 454 (59.1%, 95% CI: 0.56–0.63) tested po...
Infectious Laryngotracheitis in Layer Birds from Tamil Nadu, India
LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, 2020
Infectious laryngotracheitis (ILT), caused by Gallid herpesvirus1, is one among the respiratory diseases of poultry gradually spreading worldwide, including Indian subcontinent. Present study was carried out to identify the pathogen from the suspected cases of the disease. The tracheal tissue samples (pooled) were collected from the birds suspected to have died of ILT from 26 commercial poultry farms located in and around Namakkal district of Tamil Nadu state of India. On post-mortem examination, haemorrhagic caseous exudate and fibrinous pseudo-membrane adhered to tracheal mucosa were noticed. A total of 22 farms were found positive through PCRs targeted against ICP4 gene and the thymidine kinase (TK) gene followed by confirmation through sequencing. Histopathology examination revealed decilliation, hyperplasia, degeneration and/or loss of tracheal epithelium with severe submucosal edema. There was infiltration with numerous lymphocytes, macrophages and plasma cells in milder infec...
Tropical veterinarian, 2018
Since 2003, India has had a well-established influenza surveillance network, though Influenza C virus was not the focus of study. We therefore retrospectively analyzed clinical samples from Pune, western India collected during January 2009 to August 2015, by real-time RT-PCR. Three of 2530 samples of patients with influenza-like illness (ILI) or severe acute respiratory illness (SARI) showed positivity for Influenza C virus infection, while 105 and 31 samples were positive for Influenza A and B viruses respectively. Influenza C viruses were successfully isolated using the embryonated egg system and whole genomes were sequenced and analysed phylogenetically.
Avian Diseases, 2009
; laryngotracheitis (ILT) is a respiratory disease of poultry caused by an alphaherpesvirus (ILTV). To evaluate differential detection of ILTVs belonging to the two types, wild-type or vaccine-type, both causing clinical signs, five PCRs were evaluated to detect wild-type and vaccine-type ILTV in clinical samples. By directly sampling the organs, we aimed to avoid changes in the virus genome and to facilitate a fast diagnosis. The samples were tracheal and spleen homogenates and feather shafts. The latter are easy to collect, nonlethal for the bird, and advantageous for monitoring purposes. We investigated the time interval for vaccine virus detection following commercial vaccination by the vent application, which is successfully practiced in Israel. The study indicated that ILTV amplification from feather shafts was possible in clinical cases for about a one-month period after vaccination. Vaccine strains were identified by nested PCR for the ILTV-gE gene and differed from wild-type ILTV strains by two criteria: 1) While avirulent vaccines could be detected for about a month after the vent application, wild-type virus could be detected, in conjunction with clinical signs, for an unlimited time period; and 2) The ILTV vaccine was present in the bird in minute quantities compared to the wild-type virus. We assessed the virus type that appeared in conjunction with the clinical signs and determined that the clinical signs appeared in conjunction with both molecular forms of ILTV.
Isolation and Characterization of Infectious Laryngotracheitits Virus in Layer Chickens
The present research work was conducted for the isolation and characterization of infectious laryngotracheitis (ILT) virus in layer chickens from commercial farms of Gazipur District. A total of 25 field samples were collected from suspected layer chickens of five commercial farms and were cultivated into 10-12 days old embryonated chicken eggs through chorioallantoic membrane (CAM) route for isolation of field virus. The field viruses were characterized by physico-chemical properties against p H , heat, ether and chloroform, serological test such as virus neutralization test (VNT) and passive haemagglutination (PHA) test and pathogenicity testing. In the embryonated chicken eggs, virus produced discrete pock lesions as early as 2 days of post inoculation and embryo death was recorded within 4-6 days of inoculation. The viruses could be inactivated by p H 4 within 2 hours. Inactivation of viruses was observed at 60 0 C for 6 minutes, 55 0 C for 15 minutes and 38 0 C for 2 days. Etherchloroform treatment also inactivated the viruses. Virus neutralization test revealed that all the virus isolates were neutralized by antiserum to ILT vaccine. Passive haemagglutination test showed that the tanned sheep RBC sensitized with the virus isolates were agglutinated in presence of the antiserum to ILT vaccine. The pathogenicity test recorded 100% mortality in experimental chickens. Data of this study suggest that the field isolates might be infectious laryngotracheitis virus.
Indicators and risk factors of infectious laryngotracheitis in layer hen flocks in Algeria
Veterinary World, 2021
Background and Aim: Since 2017, there have been epidemics with respiratory disorders in the laying hen farms in Algeria, as signs and lesions, respiratory difficulties, and hemorrhagic tracheitis, which closely like laryngotracheitis. This study aimed to analyze the epidemiological, serological, and clinical indicators, as well as the risk factors, of infectious laryngotracheitis (ILT) in layer hen flocks in Algeria. Materials and Methods: A total of 1728 layer hens were sampled randomly from 48 poultry houses. Blood samples were collected from each hen at the wing vein area, and an indirect enzyme-linked immunosorbent assay was done using an IDvet® kit. Results: The flocks showed 56.25% seroprevalence. Clinical signs and gross lesions of ILT suspect cases included respiratory signs characterized by hemorrhagic tracheitis and sinusitis; conjunctivitis; egg drop; and a low mortality rate varying from 5% to 20%. Statistical analyses showed the effect of risk factors on the seropositivity for ILT in 48 layer flocks. When the vaccination was not applied, flocks were significantly more seropositive by 54% (odds ratio OR=1.54, p=0.01) compared to vaccinated flocks. Furthermore, flocks with poor hygiene were more seropositive by 68% (OR=1.68, p=0.002) compared to those with good hygiene. Finally, flocks with decreased egg production between 10% and 30% were significantly more seropositive by 42% (OR=1.42, p=0.04) than those with egg production >30%. Conclusion: The serological survey revealed anti-ILT virus antibodies, signifying the circulation of this virus in layer hen farms in Algeria. Correct vaccination protocol, strict biosecurity measures, rapid diagnosis, and detection of latent carriers are necessary to control and eradicate the disease from layer farms.