An evaluation of the activity of biologically synthesized silver nanoparticles against bacteria, fungi and mammalian cell lines (original) (raw)
2020, Colloids and Surfaces B: Biointerfaces
Biogenic silver nanoparticles due to their size, surface characteristics and strong antimicrobial properties are getting much attention for various applications. The present study aimed to investigate the synthesis of silver nanoparticles from the indigenous strain Bacillus sp. MB353 (PRJNA357966). Detailed characterization of silver nanoparticles was performed by UV-Vis Spectrophotometer, FTIR, SEM and XRD. Biogenic silver nanoparticles were crystalline with average size 49-53 nm. These silver nanoparticles possessed good antibacterial activity against Gram positive and Gram negative bacteria (E. coli, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecium, Enterococcus faecalis and Streptomyces laurentii). The nanoparticles showed excellent antifungal activity against Aspergillus niger, Aspergillus fumigatus, Fusarium soleni. Occurrence of negligible antioxidant activity pointed out the possible mode of antimicrobial activity in generation of reactive oxygen species and interaction of silver ions with bacterial cells. Incubation of silver nanoparticles with mammalian cell lines (Rhabdomyosarcomas) and fibroblast cells showed cell death and inhibition of proliferation. Possible mode of cytotoxicity was due to changes in ROS generation and intracellular calcium levels. These findings suggested that biogenic silver nanoparticles could be used as alternative agents for biomedical purposes such as antibacterial and antifungal agents. Similarly they could only be used as anticancer agents if applied in site-directed therapies as they inhibited proliferation of cells and induced apoptosis by generation of ROS with no ATP production required for cellular energy.
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