A rapid HPTLC method to estimate piperine in Ayurvedic formulations (original) (raw)
Related papers
Indo American Journal of Pharmaceutical Research, 2013
Objectives: A new simple spectrophotometric method for simultaneous estimation of Embelin and Gallic Acid in Vidang, Harade and Vidangadi Churna was investigated. The λmax of Embelin and Gallic Acid were found to be 341 and 258 nm. Method: The graphical absorbance ratio method was used for simultaneous estimation of Embelin and Gallic acid in Vidangadi Churna, and the Isoabsorbtive point for simultaneous estimation of both the drug were found to be 307.5 nm. The method obeys Beers law in the concentration range from 2-12 μg/ml. The correlation coefficient was found to be 0.9973 for Embelin and 0.998 for Gallic Acid. The method was validated as per ICH guidelines. Results: The result of estimation of Embelin in Vidang, Gallic acid in Harade and both in Vidangadi Churna were found to be 1.05 %, 1.04 %, and 1.85. The proposed method was validated for linearity, accuracy and application for assay as per ICH guidelines. Conclusion: The study showed good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simple, specific, precise, and accurate. Hence it can be applied for simultaneous estimation of Embelin and Gallic Acid in various Ayurvedic Ch urna formulations.
Journal of Pharmacognosy and Phytochemistry, 2018
Herbal medicines widely used in health-care in both developed as well as developing nations. Herbals are usually considered harmless and ever supplementary being consumed by people devoid of prescription. Though, some of them leads to health troubles, some are not effective and some might be interact with supplementary drugs. Consequently, standardization of herbal formulations is essential in order to estimate the quality of drugs, based on the concentration of their active principles. In the present study, Shatavari based Galactogogue, herbal formulation manufactured by Fredun Healthcare Pvt. Ltd., Mumbai is used for standardization. The HPTLC method was validated according to ICH guidelines and shown to be specific, linear, repeatable and accurate, within the established ranges. Statistical analysis proves that the method is reproducible and selective. This method can be used for the quantitative determination of piperine in herbal extract and its formulations.
Uv Spectrophotometric Determination of Piperine in Navasaya Churna: A Polyherbal Formulation
2017
Navayasa Churna is an important ayurvedic formulation, is mentioned in Bhaishajyaratnavali in Pandu roga chikitsa iscombination of Nine i.e. Amlaki, Bibhitaka, Haritaki, Marica Pippali, Sunth, Chitraka, Musta, Vidanga and Lauha bhasma. The formulation is dispensed for the treatment of Anemia (pandu), Hepatoprotective and Liver disorders. The method for spectrophotometric determination of piperine from the fruits of Piper longum, Piper nigrum and Navasaya Churna has been developed at absorption maxima 342.7nm. The concentration of piperine present in raw material was found to be 2.981 ± 0.38 % (w/w) in marica and 0.981 ± 0.047 % (w/w) in pippali, respectively and in three identical laboratory batch of Navasaya churna name NY-I, NY-II, and NY-III, was 0.223 ± 0.34, 0.219 ± 0.42, 0.215 ± 0.43 % (w/w), respectively with mean value 0.219 ± 0.903 % (w/w). The piperine content of all the three batches is found to be in close proximities with each other.
TLC Densitometric Method for the Estimation of Piperine in Ayurvedic Formulation Trikatu Churna
2011
Trikatu Churna is well known Ayurvedic Formulation, comprised of the fruits of two medicinal important plants of Piper longum (Pipali) along with Piper nigrum (Marica) and rhizomes of Zingiber officinalis (Saunth). Trikatu churna is a digestive tonic for the assimilation of other foods in the body. It is a rejuvenator and stimulant and regulates kapha and vata. Thus plays an important role in treatment of variety of conditions 1-3 .
Asian Journal of Pharmacognosy, 2019
The plant species of the family Piperaceae contain valuable natural compounds. Among the genera of Piperaceae family the Piper is an important genus which consists of 700 plant species growing in the different parts of the world. Many plants of this Piper genus are the essential medicinal plants used in various traditional systems of medicine. Among those plants fruits of Piper nigrum L., fruits and root of Piper longum L. and stems and roots of Piper retrofractum Vahl are commonly used in the Sri Lankan traditional system and Ayurveda system of medicine. Piper species have demonstrated strong efficacy against both malignant and non-malignant diseases. Piperine is a valuable alkaloid found in the fruits and roots of P. longum (long pepper), P. nigrum (black pepper) and stems of P. retrofractum. The main objective of this study was to establish the physico-chemical, phytochemical and pharmacognostical profiles of P. longum, P. nigrum and P. retrofractum in order to develop standard parameters. Standardization parameters such as ash, extractive values, heavy metals and qualitative phytochemical analysis were performed. Analysis of TLC and HPTLC fingerprint patterns of methanolic extract of 3 plants were also done. The results of ash and extractable values were comparable with the results in Ayurveda pharmacopeia of India. Phytochemical analysis reveals the presence of saponins, alkaloids, tannins, phenols, flavanoids, terpinoids, cardiac glycosides and steroids. Heavy metal concentrations were found to be within the limits. Results of these physico-chemical parameters, phytochemical, macroscopic and microscopic analysis can be used to assess the quality and detection of any adulteration for P. nigrum, P. longum and P. retrofractum.
Asian Journal of Pharmacognosy, 2019
The plant species of the family Piperaceae contain valuable natural compounds. Among the genera of Piperaceae family the Piper is an important genus which consists of 700 plant species growing in the different parts of the world. Many plants of this piper genus are the essential medicinal plants used in various traditional systems of medicine. Among those plants fruits of Piper nigrum L., fruits and root of Piper longum L. and stems and roots of Piper retrofractum Vahl are commonly used in the Sri Lankan traditional system and Ayurveda system of medicine. Piper species have demonstrated strong efficacy against both malignant and non-malignant diseases. Piperine is a valuable alkaloid found in the fruits and roots of P. longum (long pepper), P. nigrum (black pepper) and stems of P. retrofractum. The main objective of this study was to establish the physico-chemical, phytochemical and pharmacognostical profiles of P. longum, P. nigrum and P. retrofractum in order to develop standard parameters. Standardization parameters such as ash values, extractive values, heavy metals and qualitative phytochemical analysis were performed. Analysis of TLC and HPTLC fingerprint patterns of methanolic extract of 3 plants were also done. The results of ash and extractable values were comparable with the results in Ayurveda pharmacopeia of India. Phytochemical analysis reveals the presence of saponins, alkaloids, tannins, phenols, flavanoids, terpinoids, cardiac glycosides and steroids. Heavy metal concentrations were found to be within the limits. Results of these physico-chemical parameters, phytochemical, macroscopic and microscopic analysis can be used to assess the quality and detection of any adulteration for P. nigrum, P. longum and P. retrofractum.
Quantification of Piperine from Single herb formulation containing Piper nigrum by HPTLC
2016
Background: Black pepper is the important plant in Ayurveda possessing Immunomodulator property. Objectives: To develop HPTLC method for Piperine from single herb formulation. Settings and Design: A HPTLC method was developed for the determination of Piperine in Single herb formulation. Materials Methods: Analysis of Piperine was performed on HPTLC aluminium plates pre-coated with silica gel 60F254 as a stationary phase. Ascending development was carried out in twin trough glass chamber previously saturated with mobile phase consisting of toluene: ethyl acetate: formic acid (8: 3: 0.5 v/v/v) at room temperature (25±2ºC). The densitometric scanning was carried out at wavelength 340 nm by using Camag TLC scanner-3. Statistical Analysis: The RSD and Correlation coefficient (r 2) were calculated by standard formulas Results: Piperine was appeared in chromatographic zones at Rf value 0.52 for Single herb formulation. The linear calibration plots showed good results with r 2 = 0.998. Conc...
Rasayan Journal of Chemistry 01/2008; 1(2):212-7., 2008
ABSTRACT: A validated HPTLC method for the estimation of piperine in herbal cough syrup is described. Separation was achieved on pre-coated silica gel plate 60F254 using Ethyl acetate: Hexane (60: 40 v/v) as mobile phase. Quantitation was carried out by the use of densitometer in absorbance mode at 330nm. The method gave good separation of piperine at Rf 0.37 from other compounds. The linearity for piperine was found to be in the concentration range of 10-50μg/ml. The percentage w/w content of piperine was found to ...
QUANTIFICATION OF PIPERINE BY HPTLC METHOD
In present study HPTLC method has been carried out for determination of piperine of different manufactures. These days' numbers of Pharmaceutical companies are manufacturing Tribhuvankirti Rasa. In this study five samples out of which one is lab prepared (self-prepared) Tribhuvankirti Rasa and four are market preparations. The alcoholic extract of Tribhuvankirti Rasa and standard piperine samples were applied on TLC aluminium plate pre coated with silica gel 60 F 254 and developed using toluene: Ethyl acetate (7: 3) v/v as a mobile phase, using UV detector at wavelength of 254 nm. Content of marker compound in the samples were found similar.
High-performance liquid chromatography method for estimating piperine in herbal tea
INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “TECHNOLOGY IN AGRICULTURE, ENERGY AND ECOLOGY” (TAEE2022)
Herbal Tea is a polyherbal blend that is an Ayurvedic formulation. In this study, a quantitative HPLC technique for estimating piperine in a laboratory formulation of Herbal Tea was established, with detection in the ultraviolet region at 342.5 nm. Three batches of Herbal Tea formulation, HT-I, HT-II, and HT-III were prepared in the lab and their percent (w/w) piperine concentration was determined. Piperine concentrations in Piper longum (Pippali) and Piper nigrum were observed to be 1.38±0.39% (w/w) and 3.57±0.35% (w/w) respectively (Marica). Piperine concentration was reported to be 0.66±0.06, 0.71±0.09, and 0.69±0.03 percent (w/w) in HT-I, HT-II, and HT-III, respectively. The piperine LODs and LOQs were 0.021035 mg/ml and 0.051562 mg/ml, respectively.