Simultaneous Determination Of Mefenmic Acid And Paracetamol From Combined Dosage Forms By Spectrophotometry (original) (raw)
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2011
A simple, fast, precise, specific, accurate reversed phase high performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous determination of Paracetamol (PC) and Mefenamic acid (MA) in tablets. The column used was Inertsil ODS 3V C 18, 250 x 4.6mm, i.d. 5µm and a mobile phase composed of methanol: buffer (0.02MKH 2 PO 4) (75:25), pH 7.1 adjusted with 0.1N NaOH. The flow time was set at 1.0mL/min. Analysis was performed using UV detection at 275nm.. The retention times of PC and MA were found to be 2.88 mins and 4.49 mins respectively. Linearity was established for PC and MA in the range of 36-180 µg/mL and 40-200 µg/mL, respectively. Repeatability and intermediate precision were acceptable (RSD<2%). The percentage recoveries of PC and MA were found to be in the range of 98.32% to 100.78% and 98.15% to 99.02% respectively. The proposed method was validated and successfully used for estimation of paracetamol and mefenamic acid in the pharmaceut...
2011
A simple, fast, precise, specific, accurate reverse d phase high performance liquid chromatographic (HPLC) method was developed and val idated for the simultaneous determination of Paracetamol (PC) and Mefenamic aci d (MA) in tablets. The column used was Inertsil ODS 3V C18, 250 x 4.6mm, i.d. 5μm and a mobile phase composed f methanol: buffer (0.02MKH2PO4) (75:25), pH 7.1 adjusted with 0.1N NaOH. The flow time was set at 1.0mL/min. Analysis was performed using UV detection at 275nm. . The retention times of PC and MA were found to be 2.88 mins and 4.49 mins respectively. L inearity was established for PC and MA in the range of 36-180 μg/mL and 40-200 μg/mL, respect ively. Repeatability and intermediate precision were acceptable (RSD<2%). The percentage recoveries of PC and MA were found to be in the range of 98.32% to 100.78% and 98.15% to 99.02% respectively. The proposed method was validated and successfully used for esti mation of paracetamol and mefenamic acid in the pharmace...
Spectrophotometric Simultaneous Determination of Paracetamol and Aceclofeanc in Tablet Dosage Form
Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2014
A simple, precise and accurate UV spectrophotometric method was developed for the simultaneous determination of Paracetamol and Aceclofenac in tablet dosage form. The method involved solving simultaneous equations based on measurement of absorbance at two wavelengths 247nm and 276nm. The proposed method was validated for linearity, accuracy and precision. The percentage recovery was found to be 99 - 101% for Paracetamol and 98 – 100% for Aceclofenac which indicates that the method was accurate and precise for
Analytica Chimica Acta, 2009
Second-order advantage of excitation-emission fluorescence measurements was applied to the simultaneous determination of paracetamol (PC) and mefenamic acid (MF) in urine samples. Two drugs were quantified by multivariate curve resolution coupled to alternative least squares (MCR-ALS) in micellar media of sodium dodesyl sulfate (SDS). Experimental conditions including pH and SDS concentration were optimized. Under the optimum conditions, pH 2.0 and 0.05 mol L −1 of SDS, paracetamol and mefenamic acid were determined in concentration range 4.00-20.00 g mL −1 and 0.80-5.00 g mL −1 , respectively, in urine samples.
Spectrophotometric Estimation of Paracetamol in Bulk and Pharmaceutical Formulations
E-Journal of Chemistry, 2011
A new, simple and sensitive spectrophotometric method for the determination of paracetamol has been developed. The proposed method is based on the reaction of paracetamol with iron(III) and a subsequent reaction with ferricyanide in an hydrochloric acid medium to yield Prussian bluish green coloured product with a miximum absorption at 715 nm. There were no interferences observed from the common excipients present in the formulations. The method is successfully employed for the determination of paracetamol in various pharmaceutical preparations and the results have been statistically compared with those obtained by the official method.
Spectrophotometric determination of mefenamic acid in pharmaceutical preparations
Journal of Analytical Chemistry, 2008
Three simple, rapid and accurate spectrophotometric methods were developed for the determination of mefenamic acid. The first method (Method I) is based on the reaction of mefenamic acid as N-donor with p-chloranilic acid as a π-acceptor. A red colour product shows peak at 520 nm and its absorbance is linear with concentration over the range 10-300 µg/mL with correlation coefficient (n = 12) of 0.9997. The second method (method II) involves oxidation of mefenamic acid with N-bromosuccinamide. A yellow colour product shows peak at 362 nm and its absorbance is linear with concentration over the range 5-70 µg/mL with correlation coefficient (n = 8) of 0.9999. The third method (method III) is based on the formation of an oxidative coupling product by the reaction of mefenamic acid with 3-methylbenzo-thiazolin-2-one hydrazone as a chromogenic reagent in presence of ferric chloride solution. A green colour product shows peak at 602 nm and its absorbance is linear with concentration over the range 1-6 µg/mL with correlation coefficient (n = 6) of 0.9999. The different parameters affecting the reaction pathway were thoroughly studied and optimized. The developed methods could be successfully applied to the determination of mefenamic acid in either pure form and in pharmaceutical formulations. The results obtained were in good agreement with those obtained using official methods.
In the present investigation, 1.0 M urea solution was employed as hydrotropic solubilizing agent to solubilize poorly water-soluble drug paracetamol for its spectrophotometric analysis. The proposed method is new, simple, environmentally friendly, accurate, reproducible, precise and validated statistically for simultaneous estimation of paracetamol and diclofenac sodium in bulk drug and tablet dosage form. Simultaneous equation method, absorption ratio method and dual wavelength method have been used in the estimation of both the drugs. All the three methods show good accuracy and precision which was validated statistically. The optimized methods showed good reproducibility and recovery with standard deviation of < 1.0% and percent relative standard deviation less then 2.0%, standard error in case of recovery studies are satisfactorily low and allow the simultaneous estimation of paracetamol and diclofenac sodium in concentration ranges employed for this purpose in the assay of bulk drug and tablets. It is, thus, concluded that the proposed method is new, simple, cost-effective, safe, accurate, precise and environmentally friendly. This method can be successfully employed in the routine analysis of both the drugs in tablet dosage form.
2015
The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. second order derivative method for the determination of paracetamol and aceclofenac in combined dosage form i.e. tablets by using methanol as a solvent. The method was further validated by ICH guidelines. The proposed second order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 267 nm and 224 nm were selected for the estimation of paracetamol and aceclofenac respectively. The linearity of the proposed method was found in the concentration range of 1 to 12 µg /ml (r 2 = 0.9984) for Paracetamol and 1 to 14 µg /ml (r 2 = 0.9985) for aceclofenac respectively. The percentage mean recovery was found to be 99.580 % for paracetamol and 101.166 % for aceclofenac respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less perce...
The Open Analytical Chemistry Journal, 2015
A comparative study of the use of first derivative zero order crossing spectra for the resolution of Paracetamol and Tramadol hydrochloride in mixtures has been achieved showing the success of the first derivative method in resolving and quantifying both compounds. Using the first derivative, rather than the second derivative, results in improved signal to noise ratio. The absorption spectra of prepared mixtures were scanned in the range of 200-500 nm. The linear concentration ranges were 25-112 and 6-48 µg mL -1 for paracetamol and Tramadol hydrochloride, respectively. The method has been successfully used for prediction of concentrations of both compounds in mixtures with good selectivity, high sensitivity and extremely low relative error. Statistical comparison was performed using t-test at 95% confidence level. There was no significant statistical difference between the results obtained by the first derivative method and the accepted values for both compounds. Also, the percentage errors were very low which adds to the merits of our work in terms of both sensitivity and accuracy.
The validation characteristics of the spectrophotometric quantitative determination of paracetamol in tablets by specific absorbance according to the British Pharmacopoeia (BPh) have been evaluated. The results of paracetamol content of 83.59% and 84.39% in terms of the average mass of one tablet do not meet the permissible limits В ±5.0%. The peculiarities of the sample preparation method for quantitative determination of the active pharmaceutical ingredient in tablets has been discussed, and comparative analysis of “Dissolution” and “Assay” tests for paracetamol tablets according to the BPh has been conducted. We have suggested to make such changes at the stage of the sample preparation as “… place the flask in an ultrasonic bath for 30 min…” instead of “…shake for 15 minutes…”. The acceptance criteria of the assay method for paracetamol tablets have been calculated for permissible limits of ±5.0%, ±7.5%, ±10.0%. The results of the convergence and linearity research of the method meet requirements for the permissible limits of ±5.0%. The results of the intermediate precision re- search of the method meet requirements for the permissible limits of ±7.5%. The results of the accuracy research of the method meet requirements for the permissible limits of ±10.0%. Taking into account the technical capabilities of the Ukrainian producers and a diverse list of excipients used in the manufacture of the drug, the spectrophotometric quantitative determination of paracetamol tablets by specific absorbance is recommended to use with the permissible limits of ±10.0%. The prognosis of the total uncertainty of the analysis results is consistent with requirements to the maximum permissible uncertainty of the analysis = 2.6 ≤ max = 3.2% and with results of the 3rd round of the Professional Testing Programme (PTP) of “Pharma-test” laboratories in the system of the State Inspection for Medication Quality Control of the Ministry of Public Health of Ukraine.