Abstract 463: Glucose-regulated protein 94 modulates the response of osteosarcoma to chemotherapy (original) (raw)

2018

Abstract

Background: Osteosarcoma (OS) is the most common and most aggressive primary solid malignant bone tumor in children and young adults and has high rates of recurrence and metastasis. The endoplasmic reticulum (ER) stress pathway is important in regulating chemo-responsiveness in cancer. However, the role of glucose-regulated protein 94 (GRP94) in regulating the chemotherapy response of OS has never been explored. Method: In this study, GRP94-knockdown (GRP94-KD) MG63 OS cells were generated using short hairpin RNAs, and the response to chemotherapy was assessed by an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Results: GRP94-KD cells were shown to be more resistant to chemotherapy than scrambled control cells, and the scrambled control cells had higher levels of apoptosis after chemotherapy than GRP94-KD cells. Conclusion: Therefore, silencing GRP94 may increase resistance to chemotherapy treatment in OS cells by inhibiting the activation of apoptosis. Thus, GRP94 may be a key biomarker for the chemotherapeutic response of OS. Citation Format: Huang Chien-Yu, Po-Li Wei, Yu-Jia Chang. Glucose-regulated protein 94 modulates the response of osteosarcoma to chemotherapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 463.

Po-Li Wei hasn't uploaded this paper.

Let Po-Li know you want this paper to be uploaded.

Ask for this paper to be uploaded.