Investigation of physico-chemical properties and evaluation of the biological potential of essential oil extracted from Artemisia pallens (original) (raw)
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Molecules
Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fresh leaves of A. vulgaris grown in Manipur. The AVEO isolated by hydro-distillation from A. vulgaris were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component...
Essential oil (E.O.) of Artemisia herba-alba Asso. (Asteraceae) known as Shih, growing wild in Hermel (Lebanon) was obtained by hydrodistillation and analyzed by GC and GC-Mass spectrometry (MS). The results were α-Pinene (45.89%) followed by Borneol (11.3%), 1,8-Cineole (10.8%), Terpineol (6.45%), Camphene (3.94%), γ-Terpinene (3.2%), α-Terpinene (2.72%), (+)-4-Carene (2.2%). The antimicrobial activities of E.O. from Artemisia herba-alba Asso were evaluated against five bacteria and two fungi strains by disc diffusion method and microdilution method. Among Gram positive bacteria growths, S. aureus was shown to be more sensitive (90 mm) E. faecalis (25 mm). On the other hand, three different Gram negative bacterial strains were tested,S. enteritidis showed maximum zone of inhibition (40 mm), followed by E. coli (26 mm) and P. aeruginosa (25 mm) respectively. Theinhibition zones displayed by fungalstrains tested were40 mm and 30 mm for C. albicansand A. fumigatus, respectively. The results of the study showed an interesting antimicrobial profile which could provide promising pharmaceutical and economic benefits of the potential use of the plant essential oils. The present investigation supported the traditional use of Artemisia herba-alba in the Lebanese folk medicine as an antimicrobial active representative of the genus Artemisia. Artemisia herba-albaextracts should be further studied for their potential use in preventing / treating diseases cited in the traditional Lebanese medicine.
Phytochemistry, 2008
The chemical composition of essential oils isolated from aerial parts of seven wild sages from Western Canada -Artemisia absinthium L., Artemisia biennis Willd., Artemisia cana Pursh, Artemisia dracunculus L., Artemisia frigida Willd., Artemisia longifolia Nutt. and Artemisia ludoviciana Nutt., was investigated by GC-MS. A total of 110 components were identified accounting for 71.0-98.8% of the oil composition. High contents of 1,8-cineole (21.5-27.6%) and camphor (15.9-37.3%) were found in Artemisia cana, A. frigida, A. longifolia and A. ludoviciana oils. The oil of A. ludoviciana was also characterized by a high content of oxygenated sesquiterpenes with a 5-ethenyltetrahydro-5-methyl-2-furanyl moiety, of which davanone (11.5%) was the main component identified. A. absinthium oil was characterized by high amounts of myrcene (10.8%), trans-thujone (10.1%) and trans-sabinyl acetate (26.4%). A. biennis yielded an oil rich in (Z)beta-ocimene (34.7%), (E)-beta-farnesene (40.0%) and the acetylenes (11.0%) (Z)-and (E)-en-yn-dicycloethers. A. dracunculus oil contained predominantly phenylpropanoids such as methyl chavicol (16.2%) and methyl eugenol (35.8%). Artemisia oils had inhibitory effects on the growth of bacteria (Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis), yeasts (Candida albicans, Cryptococcus neoformans), dermatophytes (Trichophyton rubrum, Microsporum canis, and Microsporum gypseum), Fonsecaea pedrosoi and Aspergillus niger. A. biennis oil was the most active against dermatophytes, Cryptococcus neoformans, Fonsecaea pedrosoi and Aspergillus niger, and A. absinthium oil the most active against Staphylococcus strains. In addition, antioxidant (beta-carotene/linoleate model) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were determined, and weak activities were found for these oils.
Separations
In Morocco, the endemic Artemisia herba-alba is well known by its traditional uses and health benefits. The search for natural, safe, and effective antibacterial and antifungal agents from plants is in high demand due to microbial and fungal resistance to conventional synthetic antibiotics and antifungal drugs. In this study, the A. herba-alba was collected from the region of Fez-Boulemane during the periods of March, June, and September. Essential oils (EOs) were extracted from the aerial part of the plant by the hydrodistillation method. The chemical constituents were determined using GC-MS as analytical tools. The antimicrobial activities of different oils were tested using the macrodilution method. The results showed the difference in the yields between the three EOs (0.49, 1.74, 1.30% (mL/100 g)), respectively, as well as in their corresponding chemical compositions. The main constituents revealed by GC-MS are higher contents of oxygenated monoterpenes (84.7, 84.4, 81%), such a...
International Journal of Biological Chemistry, 2015
Extraction of essential oils from locally available plant Artemisia Herba-alba was carried out using steam-distillation method. Extracted oils were screened for their chemical composition, antibacterial and antioxidant activities in order to find new metabolite products, which are characterized by a biological activity. Thirty-three constituents, representing 97.54% of the essential oil of Artemisia herba-alba was determined by GC-MS analysis. The main compounds identified are: davanone (42.8%), camphor (15.96%) and thujone (9.63%). The antimicrobial activity of the oil was tested using the agar disc diffusion method, by determining the inhibition zone and the minimum inhibitory concentration. The results have shown a great potential of the antimicrobial activity against the tested strains with an enhanced sensitivity towards the gram-negative strains of Salmonella enteric, Klebsiella and the gram-positive strains of Listeria monocytogenes and Staphylococcus sp. in comparison with the other tested bacteria. The test's results of the essential oil's antioxidant activity, obtained with the anti-radical method 2,2-diphenyl-1-1-picrylhydrazil (DPPH) and the iron-reducing power (FRAP), were compared with those of the ascorbic acid; the usual synthetic antioxidant. The comparison have demonstrated, firstly with the DPPH method a considerable level of antioxidant activity of the essential oil (IC 50 = 17.73±0.29 µg mLG 1) but still lower than that found for the synthetic antioxidant that is the ascorbic acid while the opposite occurred with the iron reduction method, with an higher obtained value of EC 50 =12.53±0.25 µg mLG 1 , for the essential oil's antioxidant activity.
The essential oil isolated from Turkish tarragon (Artemisia dracunculus) by hydrodistillation was analyzed by GC-MS. Thirty compounds representing 99.5% of total oil were identified. The predominant components in the oil were (Z)-anethole (81.0%), (Z)--ocimene (6.5%), (E)--ocimene (3.1%), limonene (3.1%), and methyleugenol (1.8%). The antibacterial and antifungal activities of the essential oils isolated from A. dracunculus, Artemisia absinthium, Artemisia santonicum, and Artemisia spicigera oils were also evaluated. In general, the oils exhibited potent antifungal activity at a wide spectrum on the growth of agricultural pathogenic fungi. Among the oils, the weakest antifungal activity was shown by the oil of A. dracunculus. In many cases, the oils of A. absinthium, A. santonicum, and A. spicigera completely inhibited the growth of some fungal species. As compared with antibacterial activities of all of tested oils, A. santonicum and A. spicigera oils showed antibacterial activities over a very wide spectrum. However, the essential oils tested showed lower inhibition zones than the inhibition zones of penicillin. In addition, antioxidant and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of tarragon oil were determined, and weak antioxidant and DPPH radical scavenging activities were found in comparison to butylated hydroxytoluene.
The Artemisia L. Genus: A Review of Bioactive Essential Oils
Molecules, 2012
Numerous members of the Anthemideae tribe are important as cut flowers and ornamental crops, as well as being medicinal and aromatic plants, many of which produce essential oils used in folk and modern medicine and in the cosmetics and pharmaceutical industry. Essential oils generally have a broad spectrum of bioactivity, owing to the presence of several active ingredients that work through various modes of action. Due to their mode of extraction, mostly by distillation from aromatic plants, they contain a variety of volatile molecules such as terpenes, phenol-derived aromatic and aliphatic components. The large genus Artemisia L., from the tribe Anthemideae, comprises important medicinal plants which are currently the subject of phytochemical attention due to their biological and chemical diversity. Artemisia species, widespread throughout the world, are one of the most popular plants in Chinese traditional preparations and are frequently used for the treatment of diseases such as malaria, hepatitis, cancer, inflammation and infections by fungi, bacteria and viruses. Extensive studies of the chemical components of Artemisia have led to the identification of many compounds as well as essentials oils. This review summarizes some of the main reports on the chemistry and anti-infective activities of Artemisia. Li. essential oils from the data in the recent literature (2000-2011).
Digest Journal of Nanomaterials and Biostructures
This study examines the essential oil and the ethanol extract of Artemisia alba Turra herb, in terms of chemical composition, antioxidant and antimicrobial activities in order to select the active pharmacological isolate for making phytopreparation from the group of bitter aromatics and/or bitter liqueurs. Chemical composition of the essential oils and volatile fractions of the extract was determined by GC-FID/GC-MS and non-volatile fractions of the extract by HPLC. The content of total phenolics and flavonoids in the extracts was determined by Folin-Ciocalteu reagent and aluminum chloride, respectively. The antioxidant activity of isolates was tested by FRAP and DPPH tests and the antimicrobial by microdilution method. Major constituents in the essential oil were camphor, artemisia ketone and 1,8-cineole (23.7%, 15.2% and 14.1%, respectively), and in the volatile fraction of the extract, scopoletin (14.0%) and corymbolone (10.3%). The main components in non-volatile fraction of the...
Phytochemical and Antibacterial Activity of The Essential Oil of Artemisia herba-alba from Morocco
2016
The extraction of essential oils from the aerial part of Artemisia herba-alba is obtained by hydrodistillation and analyzed by gas chromatography coupled with mass spectrometry (GC/MS) for determining their chemical composition. Their antibacterial activity was studied in vitro on two bacterial strains: Salmonella typhi and Staphylococcus aureus. The essential oil yields of the studied plant were 0.21 for fresh aerial part and 0.25% for dry aerial part. The major component of the essential oil from dry aerial part of Artemisia herba-alba was the α-terpineol (47.33%), myrtenyl acetate (22.22%) and chrysanthenyl acetate (20.55%). While the major compounds of essential oil from fresh aerial part was the Borneol (35.68%), αterpineol (33.36%), and δ-cadinol (12.07%). The bacterial strains tested were found to be sensitive to essential oils studied and showed a very effective bactericidal activity with minimum inhibitory concentration (MIC) ranging from 0.125 to 0.5 mg/ml.
Industrial Crops and Products, 2012
Hydrodistilled volatile oil obtained from the aerial parts of Artemisia annua L., cultivated near Sarajevo, Bosnia, was analyzed by GC–MS. More than one hundred compounds were identified, representing 95.5% of the total oil. The major constituents of essential oil were oxygenated monoterpenes, artemisia ketone (30.7%) and camphor (15.8%). Isolated essential oil was tested for radical-scavenging ability using the stable DPPH radical, the ABTS radical, for reducing power ability with a test based on the reduction of ferric cations, for reducing ability of hydroxy radical in ORAC assay, and for metal chelating ability using the ferrozine assay. In all tests oil did not show a prominent antioxidant activity, but still comparable with thymol, an already known antioxidant. The screening of antimicrobial activity of oil was individually evaluated against representatives of Gram-positive, Gram-negative bacteria and fungi, using the agar diffusion method. All tested microorganisms were inhibi...