The Initiation of Bone Formation Induced Intrinsically by Osteoinductive Hydroxyapatites (original) (raw)

The initiation of new bone formation within the porous spaces of hydroxyapatite (HA) implants involves the expression of osteogenic markers belonging to the TGF-β superfamily. To study the genetic expression of these osteogenic markers in relation to the type of HA implant used and implantation period, five different types of porous HA biomaterials were implanted in the rectus abdominis muscles of adult baboons Papio ursinus, and were harvested at two, three and 12 months. The total RNA of all harvested samples was extracted and analysed using the Northern blot technique. The results showed that Collagen type IV, GDF-10 and BMP-7 were expressed at the early time points at relatively high levels, and their expression levels were significantly reduced at 12 months. The expression of these makers was not affected by the type of porous HA implant used. The histological sections of these specimens at two and three months showed vascularised connective tissue within the porous spaces of the implants with no bone formation. However, at 12 months there were substantial amounts of bone formed in all the studied implants. The down-regulation of the expressed osteogenic markers at 12 months correlates to the amount of bone formed, suggesting some negative feedback mechanism which may be acting via inhibitory Smads proteins in relation to the amount of bone formed. Neither TGF-β1 nor BMP-3 messages were detected in any of the studied samples, It is v possible that these bone markers are not expressed locally within the vicinity of the porous HA implants but are adsorbed to the HA implants from the circulatory system. vi ACKNOWLEDGEMENTS I would like to thank: My supervisor, Professor Ugo Ripamonti, for his valuable guidance and inspiration in the creation of this project. Dr Lentsha Ramoshebi, my co-supervisor, for his help and critical input in experimental procedures and write-up. Dr Laura Roden, my co-supervisor, for her valuable scientific guidance at a difficult time in the project. Mrs. Thato Chidarikire, for her endless coaching throughout this project and for being a friend. Miss. Portia Khoboko and Miss. Nina Lewin, for being good supportive friends at a difficult time in the project. Mr. Malefetsane Sechaba Khoali, my husband, for his love, patience, emotional and financial support throughout this project.