Expression of inflammatory genes in the colon of ulcerative colitis patients varies with activity both at the mRNA and protein level (original) (raw)

Methods: 65 patients with mild, severe or remission stage UC, and 22 normal colon mucosal biopsies from control individuals were included in the study for measuring the expression of nucleotide binding oligomerization 1(Nod1) and related pro-and anti-inflammatory cytokines using quantitative reverse transcription-PCR (qRT-PCR). mRNA expression levels were then correlated with severity of disease. In order to check their expression at the protein level, immunohistochemistry (IHC) was performed using Nod1, TNF-␣, IFN-␥, IL-17, IL-23 and IL-13 antibodies.