Antimicrobial and Mechanical Properties of GIC Incorporated with Silver Vanadate Nanoparticles: An In-vitro Study (original) (raw)
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Journal of Esthetic and Restorative Dentistry, 2017
Objectives: To study the effect of silver nanoparticles incorporation to glass ionomer cement (GIC) on the Staphylococcus aureus biofilm in terms of bacterial growth and evaluate the incorporating effect on hardness and compressive strength. Methods: Silver nanopowder was added in concentration 0, 1, 3, and 5 wt% to the conventional powder of GIC Fuji IX GP and then the powder is added to the liquid and mixed together with the recommended Powder/liquid ratio of 3.6:1 g. One hundred and twenty disc and cylindrical-shaped specimens were prepared using teflon molds. The specimens were put in tissue culture plate wells contained S. aureus in brain-heart infusion broth. The plate was incubated at 378C for 24 h. Specimens were then washed, fixed, dehydrated, and air dried. The spatial distribution of biofilm was examined via scanning electron microscope. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also evaluated. After setting, the specimens were stored in distilled water for 24 h before testing for microhardness and compressive strength. Results: Scanning electron photomicrographs of biofilm formed on the control GIC, showed a consistent biofilm with a thick sheet of cells, whereas those formed were less dense at 3 wt% and below the detection limit at 5 wt% silver nanoparticles. MIC and MBC of S. aureus were 25 and 50 mg/mL, respectively. The microhardness and compressive strength values of tested groups showed a nonsignificant decrease from the control group, P 5 .58 and .82, respectively. Conclusion: Incorporation of silver nanoparticles with GIC can limit S. aureus biofilm formation with an insignificant effect on mechanical properties and noticeable influence on its coloration, which restrict its usage in areas where esthetic is not of major concern. Clinical significance As the modification of GIC with silver nanoparticles improved the antibiofilm properties without altering its mechanical properties, it could be used as a restoration of root carious lesion mainly in nonesthetic areas, a base under composite restorations in deep posterior cavities and as a core material in caries susceptible patients.
Anti-biofilm activity of silver nanoparticle-containing glass ionomer cements
Dental Materials, 2020
Objective. To develop a silver nanoparticle (AgNP) formulation for incorporation into glass ionomer cements (GICs) which minimises biofilm growth on restoration surfaces. Methods. GICs, Fuji IX, Ketac Molar, and Riva Selfcure were modified with 6, 10 and 24 g per GIC capsule of ␣-lipoic acid-capped AgNPs. Monoculture biofilms of Streptococcus mutans were cultured (72 h) on GIC specimens (n = 3) and biofilm accumulation was quantified using a viability stain with confocal laser scanning microscopy. Compression strength and flexural strength (CS & FS) were measured according to ISO 9917-1:2007 (n = 8, n = 25). GIC colour was measured at 0, 1, and 14 days following AgNP incorporation using a digital spectrophotometer. Silver release from AgNP-modified GIC specimens was monitored at 1, 3, 7 and 14 days using inductively coupled plasma-mass spectrometry. Results. AgNP-modified Fuji IX demonstrated the greatest reduction in biofilm accumulation, with 10 g Ag/capsule inhibiting biofilm formation by 99%. Ketac Molar and Riva Selfcure required 24 g Ag/capsule to achieve 78% biofilm reduction. AgNP-modified GICs demonstrated significantly higher CS and FS than sintered silver-containing GICs, and possessed equivalent or higher strength values when compared to unmodified GICs. The colour shades of AgNP-modified GICs were more comparable to VITA shades of non-modified GICs than were sintered silver-containing GICs. The silver (≥99.6%) remained within the GIC for at least two weeks following incorporation. Significance. AgNP-modified GICs exhibited significant antibiofilm activity and retained mechanical properties equivalent or superior to non-modified GICs. AgNP-modified GICs could reduce bacterial colonisation on and around restorations thereby reducing restoration failure caused by secondary caries.
Antimicrobial Properties of Silver-Modified Denture Base Resins
Nanomaterials
The surface quality of denture base resins allows for easy colonization by microorganisms including Candida albicans and Staphylococcus aureus, which cause major diseases of the oral cavity such as denture stomatitis. The widespread use of silver nanoparticles (AgNPs) in various fields of medicine has led to research of their possible application in dentistry, mostly in the prevention of bacterial adhesion, proliferation, and biofilm formation. The aim of the study was to synthesize cold and heat-curing denture base resins modified with AgNPs and AgCl, and evaluate the potential of the modified resins to reduce the growth of C. albicans and S.aureus. The produced material was characterized by Fourier transform infrared spectroscopy (FTIR). The antimicrobial potential of the modified material was demonstrated by the disc-diffusion method, microdilution method, and a modified microdilution method (i.e., disk-diffusion method in broth with viable counting). Spectroscopy confirmed the i...
Antimicrobial properties of acrylic resins for dentures impregnated with silver nanoparticles
Acta stomatologica Naissi, 2017
Sažetak Uvod. Poroznost i površinska adherentnost akrilata čine ih kolektorima infektivnog sadržaja iz usne duplje. To se posebno odnosi na hladno polimerizujuće akrilate, čija je struktura manje kompaktna. Cilj rada bio je ispitivanje antimikrobnog dejstva hladno polimerizujućeg akrilata nakon njegove impregnacije nanočesticama srebra. Materijal i metode. Polimernoj komponenti (prahu) hladno polimerizovanog akrilata dodate su različite koncentracije (2%, 5% i 10%) nanočestica srebra, nakon čega su napravljeni uzorci oblika diska promera 10 mm. Kao kontrola poslužio je disk od nanočestica srebra. Antimikrobna aktivnost ispitivana je disk difuzionom metodom na dva česta izazivača infekcija usne duplje-Gram pozitivnoj bakteriji, Staphylococcus aureus ATCC 25923, i gljivici, Candidi albicans ATCC2091. Rezultati su pokazali da uzorak čistog srebra, kao i uzorci polimera sa srebrom, pokazuju antibakterijsku aktivnost. Zona inhibicije rasta Staphylococcus aureusa na hranjivoj podlozi upravno je srazmerna koncentraciji nanočestica srebra u akrilatu. Sa druge strane, ispitivani uzorci nisu ihhibirali rast Candide albicans na hranjivoj podlozi. Zaključak. Nanočestice srebra u akrilatu pokazale su antibakterijsku aktivnost. Proširenje njihovog spektra delovanja, kao i mogućnost eventualne kliničke primene biće predmet budućih istraživanja.
Evaluating the Antibacterial Activity of AgGO Nanocomposite Against Clinical Isolate Bacteria
Journal of Southwest Jiaotong University, 2019
This study discusses the synthesis, characterization, and activity of silver nanoparticles, along with silver graphene oxide, against pathogenic bacteria. Silver nanoparticles are prepared by laser ablation and fabricated with silver graphene oxide. The synthesized silver nanoparticles and silver graphene oxide were characterized through UV-Vis spectrophotometer, Fourier-transforms spectroscopy, and zeta potential. The antimicrobial performance of silver nanoparticles and silver graphene oxide nanoparticles were monitored by the contradiction of gram-negative bacteria (Escherichia.coli and Acenetobacter bumannii), and gram positive bacteria: Staphylococcus aureus. Different concentrations of silver and silver graphene oxide have been investigated to monitor their antibacterial activity against bacterial isolates. The results indicate that the nanocomposites have antibacterial activity and the antibacterial effect increases with increased concentration of nanocomposites. Laser ablati...
EVALUATING THE ANTIBACTERIAL ACTIVITY OF AGGO NANOCOMPOSITE AGAINST CLINICAL ISOLATE BACTERIA
This study discusses the synthesis, characterization, and activity of silver nanoparticles, along with silver graphene oxide, against pathogenic bacteria. Silver nanoparticles are prepared by laser ablation and fabricated with silver graphene oxide. The synthesized silver nanoparticles and silver graphene oxide were characterized through UV-Vis spectrophotometer, Fourier-transforms spectroscopy, and zeta potential. The antimicrobial performance of silver nanoparticles and silver graphene oxide nanoparticles were monitored by the contradiction of gram-negative bacteria (Escherichia.coli and Acenetobacter bumannii), and gram positive bacteria: Staphylococcus aureus. Different concentrations of silver and silver graphene oxide have been investigated to monitor their antibacterial activity against bacterial isolates. The results indicate that the nanocomposites have antibacterial activity and the antibacterial effect increases with increased concentration of nanocomposites. Laser ablation method, a fast synthesis technique, has been used in the current study to prepare silver nanoparticle and silver nanoparticle-graphene oxide nanocomposites.
Food Packaging and Shelf Life, 2018
In this work, active antimicrobial films were synthetized with 1.0% to 8.0% silver ions incorporated into polyvinyl chloride (PVC), utilizing a simple solvent casting method was accomplished in a single step. The incorporation of silver in the PVC affected the thermal, structural and antimicrobial properties of the films, which were observed via different characterization techniques. The antimicrobial activity was evaluated through disk diffusion method against the following bacterial and fungi species: Bacillus subtilis, Aspergillus niger and Fusarium solani. In addition, a practical evaluation was performed in order to observe the shelf life extension of breads. PVC Ag1% exhibited better antimicrobial activity for Fusarium solani with halos (13.5 ± 0.3 mm), and PVC Ag2% for Bacillus subtilis (15.3 ± 0.7 mm). The resulting bread sample displayed an absence of microorganisms to PVC Ag1% after 15 days of storage. The results showed that the synthesis of the PVC-based films incorporated with silver nanoparticles (AgNPs) can be easily performed, resulting in films with antimicrobial activity, which may be applied to food storage at room temperature without use of a modified atmosphere. Recently, several combinations of antimicrobials have been exploited and or incorporated into different materials such as plastics, fibers and paper with the intention of increasing the shelf life, quality, and stability of foodstuff during storage (Cooksey, 2005). Among antimicrobial agents, silver (Ag) and silver salts (e.g. silver nitrate, AgNO 3) seem to present larger antimicrobial potential, mainly due to its effectiveness to a broad spectrum of bacteria, fungi and yeasts. The silver can destroy microorganisms by altering their metabolism (Lubick, 2008). This characteristic makes it widely used in both the pharmaceutical industry and in water treatment (Appendini & Hotchkiss, 2002). In the United States, the use of AgNO 3 is accepted by the FDA (Food and Drug Administration) as a food additive, and in the European Union it is accepted as a colouring agent, with no restrictions. Additionally, the European Food Safety Authority (EFSA) has a positive list of additives for food contact with restrictions (Quintavalla & Vicini, 2002). Nevertheless, one of the main requirements for the efficiency of these active antimicrobial films is the intense contact with food, which restricts the number of compounds that can be used for the film manufacture. Since direct contact must not cause any contamination or leave residuals that are harmful to human health or that, modify food properties (Robertson, 2006).
Journal of Functional Biomaterials
The aim of the study was to evaluate the antibacterial activity and surface hardness of a light-activated microhybrid composite resin modified with green silver nanoparticles (AgNPs). AgNPs were synthesized using an Equisetum sylvaticum extract and characterized through different methods such as UV-Vis, EDX, and FTIR. The obtained AgNPs were mixed with a microhybrid composite resin (Herculite XRV, Kerr Corp., Orange, CA, USA) in different concentrations: 0% (group A-control); 0.5% (group B); 1% (group C); and 1.5% (group D). A total of 120 composite resin disk-shaped samples were obtained and divided into 4 groups (n = 30) according to AgNP concentration. Each group was then divided into 2 subgroups: subgroup 1—samples were not soaked in 0.01 M NaOH solution; and subgroup 2—samples were soaked in 0.01 M NaOH solution. The antibacterial activity against Streptococcus mutans was determined using a direct contact test. A digital electronic hardness tester was used to determine the comp...
Carbohydrate Polymers, 2019
The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (< 2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10-20 nm silver nanoparticles (Ag NPs) within the agaragar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agaragar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gramnegative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag + ions is suppressed by the increase of the concentration of Mg 2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). Up to now, a variety of inorganic and organic materials (silica, zeolite, fiberglass, carbon materials, paper, different types of polymers including textile fibers, etc.) have been used to either host or support Ag NPs in order to obtain nanocomposites with antimicrobial activity
Development and Evaluation of Silver Nanosystem-based Novel Antimicrobial Dental Stone
World Journal of Dentistry, 2022
recognized to be effective antimicrobial agents and ingrained in dental and preventive operations. 7-9 However, they have not been combined into gypsum products. Laboratory personnel cannot consistently accept personnel protective appliances such as gloves, specifically being on the lathe machines; therefore, infections can be transmitted through skin abrasions or lacerations of empty parts. Several serological investigations have demonstrated that dental health care personnel have a slightly larger incidence of HBV disease than the general community. 10,11 An impression, IntroductIon Cross-infections and their control is a major issue in medical, dental, and nursing setups. A wide spectrum of diseases could be transmitted from materials of daily use. One such product is Gypsum. American Dental Association (ADA), along with Centre for Disease Control in 2003 republished changed rules for epidemic control in dental health care settings (Control and Prevention 2003).