Antimicrobial and Antioxidant Activities of Novel Marine Bacteria (Bacillus 2011SOCCUF3) Isolated from Marine Sponge (Spongia officinalis) (original) (raw)
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BIOACTIVE POTENTIAL OF SPONGE ASSOCIATED BACTERIA -A PRELIMINARY STUDY
IJPBS, 2018
The rich diversity of marine bacteria associated with marine invertebrates, including sponges has received major attention in pharmaceutical industries due to high scope for novel bioactive metabolites. In this study, bacteria associated with the sponges were isolated and characterized. The bioactive metabolites produced by these bacteria were extracted with ethyl acetate, hexane and chloroform. The bioactivity of crude metabolites was screened by using well and disk diffusion method against bacterial pathogens. A total of 70 bacteria were isolated from six different types of sponges. All the isolates were characterized and identified up to genus level. The results indicated that out of 70 bacterial isolates, only five bacterial species exhibited inhibitory activity against the tested pathogens such as Bacillus sp., E. Coli, Salmonella sp., Pseudomonas aeruginosa, Candida albicans and Aspergillus flavus and the strains were named as SgM1, SgM2, SgM3, SgM4 and SgM5. Among these, the SgM3 strain was suspected as Micrococcus sp., strain based on biochemical analysis, and it showed high inhibitory activity against bacterial pathogens, such as Bacillus sp., (19 mm), Salmonella sp., (20 mm) and Pseudomonas aeruginosa (16 mm) and also against fungus Candida albicans (7mm) both in well and disk diffusion assay. The crude ethyl acetate extract of SgM3 showed Minimum Inhibitory Concentration (MIC) of 20 µg/ml against Bacillus sp., and Salmonella sp., whereas the MIC value of crude extract of SgM1 was 20 µg/ml against Bacillus sp., and 40 µg/ml against Aspergillus flavus fungal pathogen. The Minimum Bactericidal Concentration (MBC) was 1.56 ml against both bacterial and fungal pathogens. Further, the active compound was characterized by thin layer chromatography (TLC) technique and the Rf value was 0.65 which revealed its bioactive potential. The present study reports that the sponge associated bacteria had the ability to produce a novel bioactive compound that may be a promising candidate for the therapeutic and biomedical application.
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges
2016
The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of α-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 µg/ml, there was significant α-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10µl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa . Base...
International Journal of Chemical and Pharmaceutical Analysis, 2014
Among the thirty-eight marine bacteria isolated from three sponges and three ascidians from Muttam India. Ethyl acetate extract of strain NIO MU 19.2 showed significant antimicrobial activity against human test pathogens as well as multi-drug resistant pathogens. A strain NIO MU 19.2 was subjected to morphological and biochemical characterization. In addition, fermentation media, media content and fermentation conditions were also optimized for maximum production of antimicrobial metabolite. NIO MU 19.2 was mass cultured using optimized conditions and ethyl extract of broth was further fractionated with column chromatography. Through bioassay-guided fractionation, active fraction was identified and subjected for chemical studies such as HPLC, UV, IR, and NMR. Further studies are in progress in order to elucidate the structure and mode of action of bioactive molecule.