Antimicrobial Activity of Fractions and the Extract from Gentiana Asclepiadea L. Underground Parts with Molecular Docking Analysis (original) (raw)

2022, Acta Medica Medianae

The willow gentian (Gentiana asclepiadea L.) is a valuable source of secoiridoids, Cglycosylated flavones and xanthones used empirically in the treatment of liver and gastrointestinal disorders. Guided by ethnopharmacological data on the use of G. asclepiadea underground parts in the treatment of diarrhea, antimicrobial activity against selected pathogens of gastrointestinal significance was examined. Presented study was aimed to evaluate antimicrobial activity of the aqueous-ethanolic extract of G. asclepiadea underground parts and its petroleum ether, ethyl acetate, butanol and water fractions. A molecular docking analysis was performed as well. The antimicrobial activity against pathogens related to gastrointestinal disorders was tested by a microdilution method. The ethyl acetate fraction showed the greatest antimicrobial activity. The lowest MIC of 0.78 mg/ml was observed against Bacillus cereus and Staphylococcus aureus, achieved by the petroleum ether and ethyl acetate fractions, respectively. The greatest bactericidal activity (MBC of 0.78 mg/ml), achieved by the ethyl acetate fractions, was recorded against Enterococcus faecalis. The yeast Candida albicans was the most resistant against the fractions and the extract. C-glycosylated flavones isoorientin and isovitexin showed the best binding affinity on Enterococcus faecalis lipoate-protein ligase A as determined by a molecular docking analysis. Considering the results of our study, underground parts of G. asclepiadea could be used as a valuable natural source of secondary metabolites with promising antimicrobial activity.

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