Effect of copper and zinc on the in vitro regeneration of Rauvolfia serpentina (original) (raw)
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Morphogenic responses of Rauvolfia tetraphylla L. cultures to Cu, Zn and Cd ions
The present work was undertaken to study the effect of heavy metals on morphogenic response from nodal explants of Rauvolfia tetraphylla L. The approach has been found to be significant for enhanced multiple shoot induction on Murashige and Skoog (MS) medium containing various concentrations of copper (Cu), zinc (Zn) and cadmium (Cd). Among various concentrations used, the optimum regeneration was recorded on MS medium supplemented with 10 lM 6-benzyladenine, 0.5 lM anaphthalene acetic acid, and 100 lM zinc sulphate which exhibited 12.2 shoots per explant with 2.5 cm shoot length after 12 weeks of culture. In case of copper sulphate, the optimum concentration was found to be 20 lM with 11.6 shoots per explant and 3.3 cm shoot length. Cadmium chloride was found to be most toxic as it showed negative effect on shoot regeneration. Healthy growing in vitro microshoots (3 cm) rooted efficiently on MS medium supplemented with indole-3-butyric acid (0.5 lM) which induced 4.8 roots with 1.8 cm root length after 4 weeks. Regenerated plantlets were successfully hardened off in Soilrite followed by their transfer to garden soil under normal sunlight.
In vitro regeneration protocol of Rauvolfia serpentina L
Bangladesh Journal of Scientific and Industrial Research, 2018
An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018
2008
The objectives of this research were to study the effects of Cu2+ addition on the reserpine production and callus growth from in vitro culture indian snake root (Rauvolfia serpentina (L.) Benth. Ex Kurz). This research frame work was based on the potency of snake root which was many exploited as anti-hypertension. The addition of elicitor Cu2+ in the form of CuCl2 would influence the ion transport of cell and changed of cytoplasm pH, and also has effects on synthesis and activity of enzymes which role in reserpine production and callus growth. The research was conducted in two steps, using Completely Randomized Design. The first step was the callus initiation to promote callus growth. Second step was the treatment to induce reserpine production. The callus was divided into five groups: 0; 5; 10; 20; 40; and 80 µM. Morphology, wet weight, dry weight, growth rate, and reserpine content of callus were determined after 15 treatment day. Data were analyzed using ANOVA and continued by DM...
Bangladesh Journal of Botany, 2020
Highest frequencies (75%) of friable embryogenic nodular calli were recorded from the root explants in the presence of MS + 4.0 mg/l BAP and 4.0 mg/l NAA. In combinations of BAP and NAA 45.4% callus produced direct plantlets formation and 36.3% only from aerial part of shoots. MS medium with 1.0 mg/l BAP and 0.5 mg/l Kn produced compact callus where 13.3% produced direct plantlets formation and 45.7% produced only shoot formation. Well-developed globular somatic embryos were found to form when the callus were cultured more than 6 - 8 weeks on MS with 1.0 mg/l BAP and 0.5 mg/l Kn. After acclimatization the well rooted plantlets were transferred to pot and grown successfully in soil.