SECONDARY METABOLITE FROM MANGROVE ENDOPHYTIC FUNGUS Fusarium proliferatum AED3 (original) (raw)

Mangrove-Associated Fungi: A Novel Source of Potential Anticancer Compounds

Journal of fungi (Basel, Switzerland), 2018

Cancer is the second leading cause of death worldwide, and the number of cases is increasing alarmingly every year. Current research focuses on the development of novel chemotherapeutic drugs derived from natural as well as synthetic sources. The abundance and diversity in natural resources offer tremendous potential for the discovery of novel molecules with unique mechanisms for cancer therapy. Mangrove-derived fungi are rich source of novel metabolites, comprising novel structure classes with diverse biological activities. Across the globe, coastal areas are primarily dominated by mangrove forests, which offer an intensely complex environment and species that mostly remain unexplored. In recent years, many structurally diverse compounds with unique skeletons have been identified from mangrove fungi and evaluated for their antiproliferative properties. These compounds may serve as lead molecules for the development of new anticancer drugs. Mangrove endophytes can be modulated using...

Analytical techniques for discovery of bioactive compounds from marine fungi

TrAC Trends in Analytical Chemistry, 2012

Marine fungi are a promising source of novel bioactive compounds as lead structures for medicine and for plant protection. The current analytical techniques and future perspectives of analytical methodologies are reviewed from the point of view of the discovery and the characterization of bioactive compounds isolated from marine fungi. This critical overview also includes a general assessment of sampling and preparation of extracts, the comparison of different methods used for separation and isolation, as well as different strategies used for structural characterization of the bioactive compounds. The evolution of the application of the bioassays for discovery of bioactive compounds is also ascertained. Finally, this review addresses the advantages and disadvantages of such techniques, and comments on future applications and potential research interest within this field.

Molecular and Biological Investigation of Isolated Marine Fungal Metabolites as Anticancer Agents: A Multi-Target Approach

Metabolites

Cancer is the leading cause of death globally, with an increasing number of cases being annually reported. Nature-derived metabolites have been widely studied for their potential programmed necrosis, cytotoxicity, and anti-proliferation leading to enrichment for the modern medicine, particularly within the last couple of decades. At a more rapid pace, the concept of multi-target agents has evolved from being an innovative approach into a regular drug development procedure for hampering the multi-fashioned pathophysiology and high-resistance nature of cancer cells. With the advent of the Red Sea Penicillium chrysogenum strain S003-isolated indole-based alkaloids, we thoroughly investigated the molecular aspects for three major metabolites: meleagrin (MEL), roquefortine C (ROC), and isoroquefortine C (ISO) against three cancer-associated biological targets Cdc-25A, PTP-1B, and c-Met kinase. The study presented, for the first time, the detailed molecular insights and near-physiological...

New secondary metabolites from the mangrove-derived fungus Aspergillus sp. AV-2

Phytochemistry Letters, 2019

Chemical investigation of the mangrove-derived fungus Aspergillus sp. AV-2 following fermentation on solid rice medium led to the isolation of a new phenyl pyridazine derivative (1) and a new prenylated benzaldehyde derivative, dioxoauroglaucin (2), together with fourteen known compounds (3-16). Chemical structures of the new compounds were unambiguously determined based on HRESIMS and extensive 1D and 2D NMR spectroscopic analyses. Compounds 2-7, 8 and 13 were assessed for their antiproliferative activity against Caco-2 cell lines, where flavoglaucin (6) revealed the most potent cytotoxicity with IC 50 of 2.87 μM. 2. Results and discussion Compound 1 was isolated as faint yellow oil, HRESIMS revealed a

Bioactive Secondary Metabolites from the Mangrove Endophytic Fungi Nigrospora oryzae

Indonesian Journal of Chemistry, 2021

Mangrove forest has a distinctive habitat adapting with marine and terrestrial environment. Chemical investigation of the extract from mangrove endophytic fungi Nigrospora oryzae had resulted in the isolation of sterigmatocystin ( 1 ) and pestalopyrone ( 2 ). The structure of sterigmatocystin ( 1 ) and pestalopyrone ( 2 ) were elucidated using mass, UV and NMR spectrometers together with the comparison with the literature data. The study also showed that sterigmatocystin displayed moderate cytotoxicity but it could be further developed as antiviral and antibacterial agent based on the SAR information reported from its analogue and derivatives.

Bioactive endophytic fungi isolated from Caesalpinia echinata Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium sp

Memórias do Instituto Oswaldo Cruz, 2015

65 online | memorias.ioc.fiocruz.br Bioactive endophytic fungi isolated from Caesalpinia echinata Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium sp. Aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native Brazilian tree Caesalpinia echinata Lam. (Fabaceae). We tested their ethyl acetate extracts in several in vitro assays. The organic extracts from three isolates showed antibacterial activity against Staphylococcus aureus and Escherichia coli [minimal inhibitory concentration (MIC) 32-64 μg/mL]. One isolate inhibited the growth of Salmonella typhimurium (MIC 64 μg/mL) and two isolates inhibited the growth of Klebsiella oxytoca (MIC 64 μg/ mL), Candida albicans and Candida tropicalis (MIC 64-128 μg/

Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans

Evidence-based Complementary and Alternative Medicine, 2022

e Sundarbans, a UNESCO world heritage site, is one of the largest mangrove forests in one stretch. Mangrove plants from this forest are little studied for their endophytic fungi. In this study, we isolated fourteen endophytic fungi from the plants Ceriops decandra and Avicennia o cinalis collected from the Sundarbans. Five of them were identi ed as Aspergillus sp. and one as Penicillium sp. by macroscopic and microscopic observation. Antibacterial activity of the crude extracts obtained from these endophytes was determined against Staphylococcus aureus, Micrococcus luteus, Escherichia coli, and Pseudomonas aeruginosa using resazurin-based microtiter assay. e isolated endophytes showed varying degrees of antibacterial activity with MICs ranging between 5 and 0.078 mg/mL. Molecular identi cation of the most active endophyte revealed its identity as Aspergillus fumigatus obtained from the leaves of C. decandra. Acute toxicity study of the ethyl acetate extract of A. fumigatus in mice revealed no mortality even at the highest dose of 2000 mg/kg bodyweight, though some opposing results are found in the subacute toxicity study. e extract was subjected to silica gel and Sephadex column chromatography resulting in the isolation of three pure compounds. LC-MS analysis of these pure compounds revealed their identity as fumigaclavine C, azaspirofuran B, and fraxetin. is is the rst report of fraxetin from A. fumigatus. All three identi ed compounds were previously reported for their antibacterial activity against di erent strains of both Gram-positive and Gram-negative bacteria. erefore, the observed antibacterial activity of the ethyl acetate (EtOAc) extract of A. fumigatus could be due to the presence of these compounds. ese results support the notion of investigating fungal endophytes from the Sundarbans for new antimicrobial compounds.

Marine Fungi: A Source of Potential Anticancer Compounds

Metabolites from marine fungi have hogged the limelight in drug discovery because of their promise as therapeutic agents. A number of metabolites related to marine fungi have been discovered from various sources which are known to possess a range of activities as antibacterial, antiviral and anticancer agents. Although, over a thousand marine fungi based metabolites have already been reported, none of them have reached the market yet which could partly be related to non-comprehensive screening approaches and lack of sustained lead optimization. The origin of these marine fungal metabolites is varied as their habitats have been reported from various sources such as sponge, algae, mangrove derived fungi, and fungi from bottom sediments. The importance of these natural compounds is based on their cytotoxicity and related activities that emanate from the diversity in their chemical structures and functional groups present on them. This review covers the majority of anticancer compounds isolated from marine fungi during 2012–2016 against specific cancer cell lines.

Cytotoxic secondary metabolites from mangrove-rhizosphere-associated fungus Emericella sp. strain SWR1718

Journal of King Saud University - Science, 2020

Chemical exploration of mangrove-rhizosphere-associated fungus Emericella sp. strain SWR1718 (Aspergillaceae) was performed through various chromatographic workup procedures. The achieved results afforded one new natural compound, emericelactone E (1) in addition to known compounds (2-7). The planar structures of the purified compounds were unambiguously carried out using several spectroscopic methods. Both relative and absolute configurations of compound 1 were carefully determined based on NOESY experiments, coupling constants and comparing its optical rotation with related congeners. Moreover, a plausible biosynthetic pathway of 1 and its related derivatives is reported for the first time in our study. The cytotoxic potential of isolated metabolites was assessed toward three human tumor cell lines where some of them exhibited moderate activities compared to paclitaxel as a standard anticancer.