Phytochemical analysis, antimicrobial activity and antioxidant activity of Valeriana jatamansi (original) (raw)
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International journal of Pharmacy and Pharmaceutical Sciences, 2014
Objectives: Asparagus racemosus (AR) Willd, is commonly known as Shatavari, belongs to family Liliaceae, and has potent medicinal values. The present study deals with detail Pharmacological evaluation of ethanol fraction of AR. Methods: Preliminary phytochemical screening, antimicrobial and antioxidant activity, qualitative analysis of antioxidant and flavonoid detection, total phenol and flavonoid content were measured. Results: Phytochemical screening showed the presence of various phytoconstituents like phytosterols, phenols, flavonoids, saponins and carbohydrates. Ethanol fraction of Asparagus racemosus roots have maximum relative percentage inhibition against gram (-) bacteria. The root displayed significant DPPH free radical scavenging activity against standard ascorbic acid. Thin layer chromatography of the ethanol fraction was also performed to determine the active principles. Four major spots obtained with the Rf values 0.20, 0.22, 0.24 and 0.58. Total flavonoid and phenol content was 188.3 mg/g quercetin equivalent and 165.22mg/g gallic acid equivalent respectively. Conclusion: The findings of the current study shows that Asparagus racemosus is a good source of various phytochemicals and can be useful to progress and surge further scientific investigation to prevent and management of different diseases by their medicinal properties.
Journal of Natural History Museum
Asparagus racemosus Willd. is an important medicinal plant of tropical and subtropical regions of Nepal and India. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani, and Siddha. Asparagus curillus Buch-Ham.ex Roxb. is also one of the species found in higher altitude of Nepal. Its roots are used as substitute for A. racemosus. Phytochemical investigation was done for these two species of Nepalese Asparagus as per Methodology for Analysis of Vegetable Drugs by I. Ciulei.1982. Phytochemical screening revealed the presence of coumarin, flavonoid, catecholic tannin, reducing compound in alcoholic extract of A. racemosus while its aqueous extract revealed polyuronoid, reducing compound, polyoses, saponin, gallic tannin, catecholic tannin, etc. Similarly, alcoholic extract of A. curillus revealed catecholic tannin, reducing compound and aqueous extract revealed polyuronoid polyoses, saponin, gallic ...
2017
Asparagus racemosus Willd. is an important medicinal plant of tropical and subtropical regions of Nepal and India. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani, and Siddha. Asparagus curillus Buch-Ham.ex Roxb. is also one of the species found in higher altitude of Nepal. Its roots are used as substitute for A. racemosus. Phytochemical investigation was done for these two species of Nepalese Asparagus as per Methodology for Analysis of Vegetable Drugs by I. Ciulei.1982. Phytochemical screening revealed the presence of coumarin, flavonoid, catecholic tannin, reducing compound in alcoholic extract of A. racemosus while its aqueous extract revealed polyuronoid, reducing compound, polyoses, saponin, gallic tannin, catecholic tannin, etc. Similarly, alcoholic extract of A. curillus revealed catecholic tannin, reducing compound and aqueous extract revealed polyuronoid polyoses, saponin, gallic ...
2018
Asparagus racemosus Willd belongs to family Asparagaceae is a common medicinal plant found in hilly and plane regions of India. The plant has got immense medicinal properties and because of that it has got special importance in ayurvedic system of medicine. In the present investigation attempts were made for study of phytochemical and antibacterial activity of ethanolic extract of root. The study showed presence of primary phytochemicals like steroids, alkaloids, saponins, carbohydrates, flavoniods, amino acids except tannins. Chromatographic analysis (HPTLC) showed presence of seven polyvalent phytochemical compounds with variable Rf valus and concentration. The antimicrobial study of extract showed significant growth inhibition against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis Streptococcus pyogenes, Streptococcus pneumoniae and Pseudomonas aeruginosa. The mixture of polyvalent compounds present in the extracts might be responsible for the antibacterial a...
Phytochemical, Antioxidant and Antimicrobial Screening of Roots of Asparagus racemosus Willd.
World Journal of Pharmaceutical Research, 4(4): 709-722, 2015
The study deals with qualitative and quantitative phytochemical evaluation of bioactive phytochemicals of roots of Asparagus racemosus, a traditionally acclaimed medicinal plant and antioxidant and antimicrobial assays of root extracts. The physicochemical and phytochemical analysis including quantitative estimation of total steroidal saponins, total alkaloid, flavonoids and TLC profiling of various extracts of A. racemosus roots were done by prescribed methods. The antioxidant activity of methanol extract was performed as reducing power assay and antimicrobial activity of water extract was assayed against Staphylococcus inoculums. Physicochemical studies resulted in standard value of certain quality ascertaining parameters. Phytochemical screening indicated the presence of various phytochemicals. Quantitative evaluation of major bioactive constituents revealed considerably high content of total steroidal saponins in roots. Methanol extract of roots recorded a high amount of total phenolics and resultant good reducing activity whereas water extract of roots found to exhibit antibacterial activity against Staphylococcus. Standard values for key quality parameters will be helpful in authentication of A. racemosus roots. Notable antioxidant and antimicrobial activities of root extracts provide scientific validation of traditional medicinal claims of the plant.
The aim of current study was to compare the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of roots of seven Asparagus species from NorthWest India. Total phenolic content measured by Folin-Ciocalteu method ranged from 3.85 ± 0.25 (A. racemosus) to 7.74 ± 0.03 (A. falcatus), 3.42 ± 0.14 (A. racemosus) to 7.22 ± 0.12 (A. falcatus) and 1.12 ± 0.13 (A. officinalis) to 3.93 ± 0.54 mg/g of dry weight of root powder (A. retrofractus) in methanolic, ethanolic and aqueous extracts, respectively, expressed as gallic acid equivalents. The total flavonoid content determined by Aluminium chloride method ranged from 0.61 ± 0.03 (A. officinalis) to 1.86 ± 0.0303 (A. densiflorus), 0.39 ± 0.01 (A. adscendens) to 1.63 ± 0.0403 (A. densiflorus) and 0.31 ± 0.00 (A. adscendens) to 1.51 ± 0.02mg/gDW (A. densiflorus) in methanolic, ethanolic and aqueous extracts, respectively expressed as rutin equivalents. The results showed that TPC and TFC values were highest in methanolic extracts followed by ethanolic and lowest in aqueous extracts. The antioxidant capacity in the form of DPPH was evaluated by spectrophotometric method. The lowest radical scavenging activity was recorded in A. adscendens (149.6 µg/ml), while highest in A. densiflorus (116.2 µg/ml).