Analysis of the bonding interface in human dentin of two adhesive systems with and without the use of chlorhexdine in the cementation of ceramic restorations (original) (raw)
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International Journal of Preventive and Clinical Dental Research, 2016
Context: Etching of dentin results in demineralization and activation of the dormant matrix metalloproteinases (MMPs). If the dentin bonding agent subsequently applied fails to penetrate the full depth of this demineralized zone, their collagenolytic effect results in deterioration of the resin-dentin bond over time. Anti-MMP agents like chlorhexidine (CHX) have a potential role in preventing this degradation. Aim: To evaluate the effect of 2% CHX as a rewetting agent on the bond strength of direct composite restoration to dentin. Settings and design: In vitro study. Materials and methods: Superficial dentin of the samples was exposed, etched, and divided into two groups. Group I specimens were rewet with water while CHX was used in group II. Composite cylinders of uniform dimension were built up and the specimens were subjected to shear bond strength testing immediately (at 24 hours) and after 6 months. Statistical analysis: Independent t-test to determine whether differences if present were significant. Results: Although the immediate shear bond strength values were higher for the CHX group, the difference was not statistically significant. After 6 months, the higher shear bond strength values were obtained for the CHX group and the difference was statistically significant. Conclusion: Rewetting with 2% CHX has a beneficial effect on the resin-dentin bond over a 6-month storage period.
International Journal of Adhesion and Adhesives, 2014
Since degradation of the adhesive interface plays an important role on dental restoration failure overtime, bonding protocols containing metalloproteinase synthetic inhibitors could be a valuable approach to preserve the bond strength of indirect restorations. A flat dentin surface was created on 40 recently extracted non-carious human third molars (n ¼10). Resin-composite blocks were randomly cemented using two resin cements: a self-adhesive and a conventional. A buffer-free 2% chlorhexidine digluconate solution was used as dentin pretreatment on experimental groups. Microtensile bond strength test was performed immediately and after specimen aging for two-years in artificial saliva. Fracture patterns were determined by SEM. Even after significant reduction in bonding effectiveness with aging, dentin bond strength values of the conventional resin cement remained higher compared to those of the self-adhesive resin cement especially when chlorhexidine pretreatment was performed. No statistical differences were observed between immediate and aged specimens luted with the selfadhesive resin cement. Chlorhexidine was effective to preserve dentin bond strength of indirect restorations when the conventional resin cement was used.
Effect of Replacing a Component of a Self-Etch Adhesive by Chlorhexidine on Bonding to Dentin
Brazilian Dental Journal, 2013
The purpose of this study was to evaluate the effect of replacing a component of the self-etch adhesive Adper Scotchbond SE (liquid A + liquid B) by 2% chlorhexidine (CHX) on bond strength to dentin after 1 day, 3 months or 6 months of water storage. Eight human teeth were sectioned to expose a flat dentin surface and were then randomly assigned to 2 groups. In the control group, the dentin surfaces were treated with the adhesive according to the manufacturer's instructions. In the experimental group, liquid A was replaced by 2% CHX. Next, a 6-mm-high resin composite block was incrementally built on the bonded surfaces. The restored teeth were then sectioned to produce stick-shaped specimens (cross-sectional area - 0.8 mm2). The microtensile bond strength (µTBS) was recorded, and the failure modes were assessed. Data were analyzed by two-way repeated measures ANOVA (α=0.05). Four additional teeth were processed in order to conduct a micromorphological analysis of the resin-denti...
Effect of a Chlorhexidine-containing Adhesive on Dentin Bond Strength Stability
Operative Dentistry, 2013
SUMMARY Purpose: The present study aimed to investigate a novel adhesive system containing 0.2% chlorhexidine digluconate (CHX) for its ability to improve the stability of the adhesive interface compared with the use of 2% CHX as a therapeutic primer. Furthermore, the study aimed to confirm the inhibitory properties of these CHX concentrations (0.2% and 2.0%) on dentin matrix metalloproteinase activity by gelatin zymography. Methods: Superficial dentin substrate for bonding was obtained from 120 non-carious human molars. A conventional adhesive Peak LC Bond and a CHX-containing adhesive Peak Universal Bond were used either in combination with 35% phosphoric acid (etch-and-rinse approach) or with self-etching primer (self-etch approach) for evaluation of the variables CHX treatment (2.0% therapeutic primer and 0.2% adhesive), adhesive approach (etch-and-rinse and self-etch), and storage time (24 hours and six months). A bonding jig was used to fabricate composite cylinders, which wer...
Microscopy Research and Technique, 2013
This study focused on adhesive interface morphologic characterization and nanoleakage expression of resin cements bonded to human dentin pretreated with 1% chlorhexidine (CHX). Thirty-two non-carious human third molars were ground flat to expose superficial dentin. Resin composite blocks were luted to the exposed dentin using one conventional (RelyX ARC) and one self-adhesive resin cement (RelyX U100), with/without CHX pretreatment. Four groups (n 5 8) were obtained: control groups (ARC and U100); experimental groups (ARC/CHX and U100/CHX) were pretreated with 1% CHX prior to the luting process. After storage in water for 24 h, the bonded teeth were sectioned into 0.9 3 0.9 mm 2 sticks producing a minimum of 12 sticks per tooth. Four sticks from each tooth were prepared for hybrid layer evaluation by scanning electron microscope analysis. The remaining sticks were immersed in silver nitrate for 24 h for either nanoleakage evaluation along the bonded interfaces or after rupture. Nanoleakage samples were carbon coated and examined using backscattered electron mode. Well-established hybrid layers were observed in the groups luted with RelyX ARC. Nanoleakage evaluation revealed increase nanoleakage in groups treated with CHX for both resin cements. Group U100/ CHX exhibited the most pronouncing nanoleakage expression along with porous zones adjacent to the CHX pretreated dentin. The results suggest a possible incompatibility between CHX and RelyX U100 that raises the concern that the use of CHX with self-adhesive cements may adversely affect resin-dentin bond.
Brazilian oral research
This study evaluated the effect of a 2% chlorhexidine-based disinfectant (CHX) on the short-term resin-dentin bond strength of a self-etch adhesive system to human dentin with different mineral contents. Dentinal mineralization was tested at 4 levels (sound, and after 2, 4, or 8 days of demineralization-remineralization cycles) and disinfectant at 2 levels [deionized water (DW, negative control) and CHX]. Dentin demineralization induced by pH-cycling was characterized by cross-sectional hardness (CSH). Each dentin surface was divided into halves, one treated with DW and the other with CHX (5 minutes). Each surface was bonded with a self-etch adhesive system and restored. The specimens were sectioned and subjected to microtensile bond testing. CSH and microtensile bond strength (µTBS) data were analyzed by regression analysis and ANOVA-Tukey tests (α = 5%), respectively. The groups treated with CHX resulted in mean µTBS similar to those found for the groups in which the dentin was ex...
Effect of 1% chlorhexidine gel on the bonding strength to dentin
Journal of Dental Sciences, 2010
Background/purpose: The purpose of this in vitro study was to evaluate the effect of 1% chlorhexidine (CHX) gel on dentin bond strengths of posterior composite resin applied with two different adhesive systems. Material and methods: In total, 75 extracted, caries-free human molars were used. The occlusal surface of each tooth was ground to create a flat dentin surface. Then, each tooth was mounted in acrylic. The dentin specimens were randomly assigned to five groups of 15 specimens each. In Group 1, Prime & Bond NT (PBNT) was applied; in Group 2, a 1% CHX gel + etching for 15 s + PBNT were applied; in Group 3, etching + 1% CHX gel + PBNT were applied; in Group 4, Clearfil S 3 Bond was applied; and in Group 5, 1% CHX gel + Clearfil S 3 Bond were applied. A dentine bonding system was applied to dentin surfaces, and composite cylinders were built up using a special device and then light-polymerized. Specimens were mounted and sheared using an Instron universal testing machine at a cross-head speed of 0.5 mm/min. Results: The results were recorded in megapascals. The sheared specimens were examined under a light microscope, and the type of failure (adhesive, cohesive or mixed) was recorded. Data were compared by one-way analysis of variance and Tukey's honestly significant difference tests. Means were 16.4 ± 4.1 MPa in Group 1, 16.2 ± 3.9 MPa in Group 2, 13.0 ± 4.5 MPa in Group 3, 11.9 ± 2.7 MPa in Group 4, and 11.5 ± 2.7 MPa in Group 5. The use of 1% CHX gel before acid etching was significantly higher than after etching on the shear bond strength of PBNT (P < 0.05), but did not differ significantly from PBNT alone (P > 0.05). Conclusion: Within the limitations of the present in vitro study, it was concluded that 1% CHX gel application did not adversely affect the shear bond strengths of dentin-bonding agents.
Effects of surface treatment on the microtensile bond strength of ceramic materials to dentin
Acta odontológica latinoamericana : AOL, 2007
This study evaluated the effects of distinct surface treatments on the micro-tensile bonding strength (microTBS) of different ceramic materials. The occlusal surfaces of eighteen human maxillary molars were flattened perpendicularly to the long axis and divided in groups based on surface treatment (sandblasting: s; hydrofluoric acid: a; tribochemical silica coating: t): DP-s, DP-a, DP-t, IE-s, IE-a, IE-t, IC-s, IC-a, IC-t) and ceramic materials (Duceran Plus: DP, IPS Empress 2: IE, In-Ceram Alumina, IC). Panavia F luting resins were used according to the manufacturers' instructions to bond ceramic materials to the exposed dentin specimens under a load of 7.5 N. After 3-day storage, microTBS was tested at a cross-head speed of 1 mm/min. Data were analyzed with ANOVA and Tukey's test. ANOVA results showed that the microTBS of DP and IC were significantly different. The microTBS of DP-a was significantly higher than those of DP-s and DP-t. The microTBS of IC-t was significantly...
Journal of Babol University of Medical Sciences, 2017
J Babol Univ Med Sci; 19(12); Dec 2017; PP: 36-42 Received: May 29 2017, Revised: Sep 29 2017, Accepted: Oct 28 2017. ABSTRACT BACKGROUND AND OBJECTIVE: The matrix metalloproteinase plays a significant role in the bonding stability of the composite-tooth. Chlorhexidine has been offered as matrix metalloproteinase inhibitor in current years. The aim of this study was to investigation of effect of 2% chlorhexidine on enamel microleakage of composite fillings in different bonding systems. METHODS: In this in vitro study, class V cavities were prepared on the lingual and buccal surface of forty extracted molar teeth. The cavities were etched and divided randomly to 8 groups: 1: Adper single Bond 2 (ASB), 2: Clearfil SE Bond (CSE), 3: Clearfil S3 Bond (CS3), 4: Single Bond Universal (SBU), 5: ASB + 2% Chlorhexidine (CHX), 6: CSE +2%CHX, 7: CS3 +2% CHX, 8: SBU + 2%CHX. After bonding application, the cavities were restored with Z250 composite and thermocycling procedure (5 to 55 C, 5000 cy...