Hydrothermal assisted biological synthesis of Silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against Pseudomonas sp. isolated from contact lenses (original) (raw)

Eurasian Journal of Biosciences, 2019

Abstract

Background: Biological synthesis of Silver Nanoparticles (AgNPs) by using honey and gomutra shall become biocompatible and cost effective alternative method wherein no extra reducing agents were used which may create problems for living systems and also adds extra cost to the system. Materials and Methods: The crude gomutra and purified honey (1 mg/mL) in sterile distilled water were used for the synthesis of Ag NPs by hydrothermal method. The characterization of Ag NPs was done by UV-Vis Spectroscopy and Scanning Tunneling Microscopy (STM) to study optical and morphological characteristics, correspondingly. The isolation of bacterial culture was carried out by incubation of swabs taken from contact lenses of healthy volunteers, followed by its identification through microscopic and biochemical studies. Antibacterial activity of Ag NPs was performed against Pseudomonas species by anti-well diffusion assay and broth dilution method. Studies were also performed for determination of bi...

Rabinder Henry hasn't uploaded this paper.

Let Rabinder know you want this paper to be uploaded.

Ask for this paper to be uploaded.