Environmental parameters affecting biosorption of heavy metals by mixed culture of Aspergillus niger and Aspergillus flavus in industrial wastewater (original) (raw)
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BIOSORPTION OF HEAVY METALS USING ASPERGILLUS SPECIES ISOLATED FROM CONTAMINATED SOIL.
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Bioremoval of Heavy Metals by the Native Strain Aspergillus niger
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The objective of this work was study the resistance and removal capacity of heavy metals by the fungi Aspergillus niger. We analyzed the resistance to some heavy metals by dry weight and plate: the fungi it grows in 2000 ppm of zinc, lead, and mercury, 1200 and 1000ppm of arsenic (III) and (VI), 800ppm of fluor and cobalt and least in cadmium (400ppm). With respect to their potential of removal of heavy metals, this removal efficiently zinc, (100%), mercury (83.2%), fluor (83%), cobalt (71.4%), fairly silver (48%) and copper (37%). We determine the optimal characteristics for lead, mercury, cobalt and zinc removal in dry cells. The ideal conditions for the removal of 100mg/L of the heavy metals Cr (VI) were 28 °C, pH between 4.0-5.5, 100ppm of heavy metal, and 5g of fungal biomass.
Journal of Applied Sciences and Environmental Management, 2005
Two isolates belonging to the predominant genera Aspergillus and Rhizopus isolated from agricultural field treated with sewage/ industrial effluents were selected for the biosorption potential evaluation of Cr and Cd. Pretreated, dead biomass of above fungi was used for bioadsorption experiment at pH value 4.5 with the biomass, 1-5 mg in a 100 ml metal solution of different concentration (2, 4, 6 and 8 mM) with a contact time of 18 hrs and agitation, 120 rpm. Bioadsorption of Cr ranged from 6.20-9.5 mg/g of dry mass at one or other initial metal concentrations by Aspergillus and Rhizopus sp. The bioadsorption of Cd was ranged from 2.3-8.21 mg/g. On the comparative basis Rhizopus sp. could bioadsorbed higher concentration of both metals as compared to Aspergillus sp. Bioadsorption of Cd and Cr was influenced by initial metal concentration and nature of organism. The findings revealed that fungi of metal polluted sites showed higher metal tolerance and bioadsorption capacity of chromium and cadmium. @JASEM
Factors Influencing the Process of Biosorption of Heavy Metals from Aqueous Solution
IAEME PUBLICATION, 2013
Various biological materials can be used for removing heavy metals like Cadmium, Copper, Nickel, Lead, and Zinc from aqueous solutions successfully. Biosorption is a process in which solids of natural origin are employed for binding heavy metals. It is a promising alternative method to treat industrial effluents, mainly because of its low cost and high metal binding capacity. Biosorption is possible by both living and non living biomass. A large number of micro-organisms belonging to various groups viz, bacteria, fungi, yeast, cyanobactreia and algae have been reported to bind a variety of heavy metals to different extents. The process of biosorption is dependent on various parameters such as contact time, pH, biomass concentration and temperature and of the solution. In this paper, the role of various influencing factors on the removal of heavy metals by biosorption is reviewed.
Bioremediation of Heavy Metals by using Aspergillus niger and Candida albicans
ZANCO Journal of Pure and Applied Sciences, 2023
Bioremediation is a branch of biotechnology that employs the use of living organisms, like microalgae and fungi, in the removal of contaminants, pollutants, and toxins from soil, water, and other environments. The study was design to know and evaluate the efficiency of fungi to remediate two types of heavy metals (Pb and Cd), by using different concentrations (5, 15, 35, and 50ppm). Results revealed that the lowest applied dose (5ppm) of both tested heavy metals had the highest reduction percent by using two fungal stains Aspergillus niger and Candida albicans were remove lead by 85.6 and 84.2%, while for cadmium were 80 and 78.4% respectively. Statistically significant differences (P≤0.05) were observed between control and the treatments for both tested heavy metals. Highest heavy metals removal was found at the end of experiment (20 days).
Effect of metal training on Aspergillus niger (Lin.) and its Biosorption capacity
© Serials Publications Pvt. Ltd., 2018
Currently, heavy metal pollution is becoming a drastic problem throughout the whole world. Conventional technologies to clean up the heavy metal from industrial effluent are less effective and time taking. An alternative to these technologies, biosorption is an inexpensive and promising solution for biodetoxification of the heavy metal pollution from wastewater. The filamentous fungi have great potential to produce a large amount of biomass which is used for metal adsorption. Filamentous fungi have gained an increasing, attention for removal and recovery of heavy metals due to their performance. For the present study Aspergillus niger, Van Tieghem was selected for the in vitro training and adsorption of lead. The species were selected on the basis of their growth rate and overall performance in the soil treated with different metal concentration. Potato Dextrose Agar medium was used for the in vitro training of these selected fungi against the lead sulphate (PbSO4). Training of fungi at a high concentration of heavy metal may increase their metal uptake capacity as a metal resistance mechanism. Aspergillus niger was strained to high concentration lead as lead sulphate (PbSO4) i.e., 1000 ppm, 1500 ppm, 2000 ppm, 2500 ppm. 3000 ppm, 3500 ppm and 4000 ppm by repetitive sub-culture on Potato Dextrose Agar medium containing a high level of lead metal. Different doses of fungal biomass i.e., 10 mg, 20 mg, 30 mg, and 40 mg were used for the present investigation. It has been observed that biosorption capacity increased with increase in the dose of biomass. Present work clearly signified 3000ppm the highest biosorption as 70.39% with 20 mg of fungal biomass. Freundlich' isotherm modal showed much better correlation coefficients than Langmuir's isotherm model in the examined concentration range. Biosorption found strongly dependant on the experimental parameters. Trained fungi can remove much lead from the solution than those of the non-trained fungal biomass.
Biosorption of heavy metals by Aspergillus niger
Global Environmental Biotechnology, 1997
Pesticide residue constitutes a danger to soil micro and macro fauna and flora and to humans. This study assessed the impact of the application of Aspergillus niger as a pesticide remediating agent on Lambda-cyhalothrin and some of its associated heavy metals translocated to the leaves of Lactuca sativa. A Complete Randomized Design (CRD) experiment using three Treatments was conducted. Lambda-cyhalothrin was extracted from the Samples using the multiple residue technique and its residue determined using Gas Chromatography with Pulsed Flame Photometric Detector (PFPD). The associated heavy metals were determined using the Atomic Absorption Spectrophotometric method. Data so generated were means from five replicates, and were subjected to a one way analysis of variance-ANOVA with Tukey HSD Test for differences between means at 99% confidence interval (P≤0.01). The results show that the highest mean value of Lambda-cyhalotrhrin residue in L. sativa leaves was found in Treatment A (1.50 mg/kg), a value which was significantly higher (P≤0.01) than the residue found in Treatments B (1.0 mg/kg) and C(0.02 mg/kg). Mean Lambda-cyhalothrin residue in Treatment A alone was above the Maximum Residue Level (MRL) of 1.0 mg/kg in vegetable set in the legal EU regulation.The application of A. niger also precipitated a significant reduction (P≤0.01) in the level of all the heavy metals evaluated except Arsenic. All the heavy metals evaluated were however below the WHO/FAO safe limit. The result from this study posits A. niger as a promising mycoremediation agent of pesticide pollution.
Biosorption of lead using pretreated cells of Aspergillus species
2013
Microbial bioremediation is an emerging technology for environmental cleanup. Application of living biomass for metal binding depends on nutrient type and concentration, environmental conditions and cell age. In addition, living biomass may be subject to toxic effect of heavy metals at elevated concentrations. To overcome the disadvantages; non-viable or dead biomass is preferred. To test these hypothesis three fungal strains were isolated from effluent of chemical and pharmaceutical industry using SDA agar. Identification of the above isolates was carried out and was identified to be predominant strains of Aspergillus i.e. (Aspergillus niger and Aspergillus flavus). Further preliminary test was performed to check the tolerance of the fungi to different metal salts of lead, copper, chromium, zinc, nickel, cadmium using 1mM concentration. All three fungal species showed tolerance to metal salts like lead nitrate, zinc sulphate and cupric sulphate above 20 mM. Furthermore minimum inhi...
Biosorption of cadmium and copper by Aspergillus spp. isolated from industrial ceramic waste sludge
Biological Diversity and Conservation, 2019
Under proper conditions, fungi can act as a good biosorbent for different heavy metals. In the present study, Aspergillus spp. have been isolated from ceramic industrial waste sludge and the tolerance of the fungi for copper and cadmium metals were examined. The experiments were carried out at 25 °C , pH=4-4.5 for Cu(II), pH=6 for Cd(II), biosorbent dose of 2.5 g, initial metal concentration of Cd (II) was 1 mM and Cu(II) was 5 mM. The removal efficiencies for cadmium and copper with two Aspergillus strains were found to be 90-95% and 85-90%, respectively. The sorption capacities of live and dead fungi for copper were 5.3676 mg g-1 , 18.661 mg g-1 and for cadmium were 0.1977 mg g-1 , 0.1772 mg g-1 respectively. FTIR analyses have showed that copper ions bound to vinyl compounds (950-900 cm-1) and cadmium ions bound to primer amides (1420-1400 cm-1), mostly. Considering biosorption results, Langmuir and Freundlich isotherm models have been described and it was clearly seen that none of the isotherm models have fitted the experimental data. The metal ion binding areas of the cell surface of fungi were determined by FTIR. SEM monitoring and EDX analysis were carried out. EDX results confirmed the biosorption of copper and cadmium.
Biosorption of cadmium, manganese, nickel, lead, and zinc ions by Aspergillus tamarii
In this paper, Cd 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ heavy metals ions adsorption properties of commercially obtained Aspergillus tamarii were investigated. The dead biomass was used with a batch system for experiments. The effect of the operating parameters, such as pH, temperature, agitation speed, contact time, initial metal concentration, and biomass dosage of aqueous solution containing Cd 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ was studied to find biosorption capacity. The optimum pH range for all heavy metal uptakes was 6.0. The experiments were carried out at different temperatures in the range of 20-50˚C and the maximum uptake was found to be at 25˚C. Heavy metal ion uptake increased with agitation speed until 150 rpm. After this agitation speed adsorption capacity slightly decreased. The adsorption equilibrium was obtained at 150 min contact time. At the optimal conditions, maximum uptake of Cd 2+ , Mn 2+ , Ni 2+ , Pb 2+ , and Zn 2+ was found to be 51. 69, 46.99, 58.74, 98.14, and 54.33%, respectively, by using 1.5 g biomass. The interaction between heavy metals and biomass was characterized by FTIR spectroscopy.