In Vitro Flowering of Passiflora suberosa L.1 (original) (raw)
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In vitro propagation of nodal and shoot tip explants of Passiflora foetida L.
developed an efficient micropropagation protocol by using node and shoot tip explants of Passiflora. Explants are surface sterilized and inoculated into culture medium with different concentrations of growth regulators. The MS medium supplemented with the hormone 6 -Benzyl adenine at the concentration of 1.5 mg/l was found to be ideal for optimal growth of the inoculated shoot and nodal cultures (100%) and MS medium supplemented with 1.0 mg/l IBA hormones was best suited for induction of roots in vitro (90%). The rate of successful acclimatization was about 78% in the Sand: Soil: Compost (at the ratio of 1:1:1) of the hardening process.
Germination and seedling growth of ornamental species of Passiflora under artificial shade
Acta Scientiarum. Agronomy, 2012
Passiflora morifolia, P. suberosa litoralis, and P. palmeri var. sublanceolata are three wild species with ornamental potential that occur in Brazil. These species were evaluated with the purpose of determining the effects of different shade levels on seedling emergence and initial growth. Treatment with 50% shade resulted in the highest percentage of seedling emergence and the highest emergence speed index for all three species. The 50% shade level was the best condition for growth of P. morifolia and P. palmeri var. sublanceolata seedlings because in this condition, they presented higher growth in relation to control and the other treatments. However, P. suberosa litoralis can be considered a sun species because it grew better in full sunlight and 25% shade, but its growth was stunted at 75% shade. Increased leaf area and specific leaf area were due to changes in leaf dimension and shape in response to an increase in shade levels. These observations related to the initial growth reflect the development and adaptation of adult plants to heterogeneous environments. Therefore, P. morifolia and P. palmeri var. sublanceolata seedlings adapted better to moderate shade conditions, and P. suberosa litoralis seedlings should be cultivated in full sunlight.
developed an efficient micropropagation protocol by using node and shoot tip explants of Passiflora. Explants are surface sterilized and inoculated into culture medium with different concentrations of growth regulators. The MS medium supplemented with the hormone 6 -Benzyl adenine at the concentration of 1.5 mg/l was found to be ideal for optimal growth of the inoculated shoot and nodal cultures (100%) and MS medium supplemented with 1.0 mg/l IBA hormones was best suited for induction of roots in vitro (90%). The rate of successful acclimatization was about 78% in the Sand: Soil: Compost (at the ratio of 1:1:1) of the hardening process.
Zemdirbyste-Agriculture, 2021
The aim of the experiment was to evaluate the effect of the basal medium and the type and concentration of cytokinin on direct regeneration from internodal segments of passion flower (Passiflora edulis Sims). Internodal segment explants were cultured on the Murashige and Skoog (MS) and woody plant medium (WPM) without growth regulators or supplemented with 0.5-3.0 mg L-1 of 6-benzylaminopurine (BAP), thidiazuron (TDZ) or zeatin (ZEA). The cultivation of the internodal segment explants on media supplemented with cytokinins resulted in direct organogenesis without a callus phase. The shoot formation frequency and shoot number per explant were strongly influenced by the type and concentration of cytokinin. MS medium supplemented with BAP resulted in a shoot formation frequency that was higher in comparison with TDZ but significantly lower as compared to 0.5-2.5 mg L-1 ZEA. On the other hand, on WPM supplemented with BAP the shoot formation frequency was significantly lower in comparison with that for analogous ZEA and TDZ concentrations. The shoot formation frequency and shoot number per explant were strongly influenced by interactions of the basal medium and type and concentration of cytokinin. The MS medium supplemented with 2.0 mg L-1 ZEA resulted in the highest shoot formation frequency, while the highest shoot number per explant was obtained on WPM supplemented with 1.5 mg L-1 ZEA. The highest shoot formation frequency (98.1%) with the highest number of shoots per explant (9.53) was observed on the WPM supplemented with 1.5 mg L-1 ZEA. The formed shoots were rooted on MS medium containing ½ macro and micro salts supplemented with 2.0 mg L-1 IBA (indole-3-butyric acid); about 92% of them survived and grew normally with true-to-type morphology.
Plant Cell Tissue and Organ Culture, 2011
Passiflora suberosa is used in popular medicine, improvement programs, and as an ornamental plant. The goal of this study was to establish efficient protocols for plant regeneration and callus induction from nodal, internodal and leaf segments excised from in vitro-grown plants. The different morphogenetic responses were modulated by the type and concentration of plant growth regulators, according to the basal medium and light conditions. Shoot formation occurred through three pathways: (1) development of preexisting meristems, (2) direct organogenesis, and (3) indirect organogenesis. Development of preexisting meristems was observed from nodal segments (1 shoot/explant) in response to α-naphthaleneacetic acid (NAA), picloram (PIC), and 2,4-dichlorophenoxyacetic acid (2,4-D), using two basal media (MS and MSM). Direct organogenesis in this species was obtained for the first time in this work, through shoot development from internodal segments in the presence of 6-benzyladenine (BA). The highest regeneration rates were achieved on MSM medium, regardless of the BA concentration. Indirect organogenesis was achieved from all explant types on media supplemented with BA, used alone or in combination with NAA. The highest regeneration efficiency was obtained from internodal segments cultured on MSM medium plus 44.4 μM BA. Compact, friable, or mucilaginous non-morphogenic calluses were induced by thidiazuron, PIC, 2,4-D, and NAA. High-yielding friable calluses obtained on MSM medium supplemented with 28.9 μM PIC are being used for the establishment of suspension cultures and further analysis of the production of bioactive compounds.
In vitro Micro Propagation of Blue Passion Flower (Passiflora caerulea L)
A successful in vitro culture establishment has been achieved from the leaves of blue passion flower (Passiflora caerulea L) and the regeneration of shoots was observed with and without callus development. Leaf segment of Passiflora caerulea L. formed callus when cultured on modified Murashige and Skoog (MS) medium supplemented with 0.5 mg/l NAA, 0.5 mg/l IAA, 0.5 mg/l 2-4-D, 0.5 mg/l Kinetin and 1 mg/l BAP. Direct shoot regeneration without a callus phase has also been achieved from the leaf segment in presence of 2 mg/l BAP and 1 mg/l kinetin..According to our study, a complete plant of blue passion flower along with shoots and roots was developed in around 54 days, which may take near about two seasons or sometimes a year for a seed of blue passion flower to germinate.
Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims
Journal of Medicinal Plants Research
Passiflora edulis Sims, besides having economic and social importance, is included in the medicinal plant list that is considered of interest by the Unified Health System (Sistema Único de Saúde-SUS). Due to its high nutritional value, this species is widely used in food, cosmetics and pharmaceutical industries. It also contains carotenoids, as well as passiflorine and maracugine, which have sedative effects. The aim of this research was to assess P. edulis minimum growth conditions in different concentrations and combinations of MS culture medium and sucrose for in vitro conservation. The following variables were analyzed: Plant height; number of green and senescent leaves; and number of roots. Root height means were highest in MS media containing 30 g L-1 sucrose (8.33). With regard to plant height, the best results were obtained in MS/4 media with 15 g L-1 or 30 g L-1 sucrose, which enabled the maintenance of lower plants (5.35 and 4.91, respectively) with green leaves after 120 days in vitro culture. The MS/2 media supplemented with 15 g L-1 sucrose was the best alternative for in vitro maintenance of plants with appropriate height and number of green leaves (8.22 and 3.86, respectively). Thus, we may conclude that MS/2 with 15 g L-1 sucrose represents an efficient strategy for in vitro conservation of P. edulis plants for a culture period of 120 days and 100% survival after acclimatization.
HortScience, 2018
Passiflora are ornamental plants that are appreciated as part of outdoor decor, composing pergolas and gardens, as well as in interior ornamentation where species tolerant to environments with less light availability are used. The objective of this study was to evaluate the influence of different levels of light and pot types on morphological and reproductive characteristics in Passiflora subrotunda and to support genetic breeding programs of ornamental passifloras. The conditions of 75% and 100% light favored vegetative morphological characteristics through the time (105 days). Floral characteristics also presented higher values along increasing light levels. All qualitative characters related to flower and plant coloration did not vary among genotypes. The species possesses diurnal anthesis and flowers throughout the year. Plants cultivated in concrete pots showed greater growth and flower production. Pollen grains (PGs) are large, with an isopolar form, a small polar area, and a ...
In Vitro Cellular & Developmental Biology - Plant, 2014
The present study aimed to establish a protocol for in vitro organogenesis of Passiflora setacea and to determine the genetic stability of regenerated plants. Three types of explants (leaf, hypocotyl, and root), four growth regulator combinations [Murashige and Skoog (MS) salts, MS + 6benzyladenine (BA), MS + thidiazuron (TDZ), and MS + BA + TDZ], and two light regimes (16-h photoperiod and continuous darkness) were tested. After 30 d on induction medium, the percentage of explants forming shoots was evaluated. Direct and indirect organogenesis was evident from hypocotyl-and root-derived explants, whereas only indirect organogenesis was observed from leaf explants. The presence of BA was essential for shoot formation from leaf explants and improved the response of hypocotyl segments under a 16-h photoperiod compared to the cytokinin-free control. However, after transfer to shoot elongation medium, the greatest number of elongated shoots was obtained from hypocotyl segments that had been induced on BA + TDZ medium under a 16-h photoperiod, as was also observed for root explants. Flow cytometry analysis confirmed the genetic stability of the regenerants based on DNA quantity (2C = 2.57 pg) in comparison with seed-derived plantlets (2C=2.60 pg). This is the first report on the in vitro regeneration of P. setacea.
BA and TDZ in the Morphogenesis in Vitro of Passiflora Setacea
2022
Passiflora setacea DC (Passifloraceae) is considered an important species in the genetic breeding of passion fruit. However, its use is limited due to low seed germination. This paper aimed to study the effect of cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ) on the in vitro morphogenesis of P. setacea using three explants: hypocotyl, nodal segment, and root segment. The explants were induced to morphogenesis in MS medium modified and with different concentrations of BA and TDZ. After 55 days, the percentage of calluses and shoots were evaluated, and anatomical characterization was performed. The three explants used in the in vitro morphogenesis of P. setacea showed callus and shoots formation, but in greater numbers in the nodal segments treated with BA. TDZ isolated affected equal to or less than BA on callus and shoots formation for the three explants. Direct and indirect organogenesis was observed in the three types of explants. From the results obtained for plant regener...