Agarose gel electrophoresis, Human HV1 PCR, Product size ∼440 bp (original) (raw)

PLOS ONE, 2013

Abstract

<p>a) Evaluation of different surface sterilization protocols. Lane 1: DNA ladder, 2: Ac, 3:Al, 4:Bl, 5:AlBl, 6:AlAc, 7:AcBl, 8:AlAcBl, 9,12:Extract control, 10:Alcohol, 11:Spike, 13: PCR -ve. b) Effectiveness of surface sterilization. Halite crystals (modern Saline Valley) spiked with different amounts of human DNA. Lane 1: DNA ladder, Lanes 2,3,10,11: 1.5 ng spike, Lanes 4,5,12,13: 3.0 ng spike, Lanes 6,7,14,15: 4.5 ng spike, Lanes 8,9,16,17: no spike. Lanes 2–9: no surface sterilization. Lanes 10–17 surface sterilized.</p

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