Figure S5 from Paclitaxel Reduces Tumor Growth by Reprogramming Tumor-Associated Macrophages to an M1 Profile in a TLR4-Dependent Manner (original) (raw)

Supplementary Figure S5. Generation of myeloid cell-specific TLR4 knockout mice. (A) Representative results of PCR-based genotyping: 1.The LysM-Cre heterozygous mice were detected by genomic PCR which gives raise two bands at 700-bp and 350-bp; 2. The homozygous TLR4-floxed mice were recognized by genomic PCR rendering a 285-bp band. To confirm the efficiency of LysM-Cre-mediated TLR4 loxP recombination in macrophages, BMDM from LysM-Cre +/and LysM-Cre +/-/TLR4 fl/fl mice were stimulated with LPS (100 ng/mL) or PCX (30 µM) and after 24h were measured the cytokines production in the supernatant: (B) TNF-α and (C) IL-6. All values are means ± SEM (n=3) *P<0.05