Distribution of Potato virus Y in Potato Plant Organs, Tissues, and Cells (original) (raw)
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Spread of potato virus Y in potato cultivars ( L.) with different levels of sensitivity
Physiological and Molecular Plant Pathology, 2004
The aim of this work was to correlate the appearance of the symptoms, multiplication and spread of virus after mechanical inoculation of potato (Solanum tuberosum L.) cultivars showing different levels of susceptibility and sensitivity to Potato virus Y NTN (PVY NTN ). The potato cultivars used were the resistant cultivar Sante and susceptible cultivars Igor, Pentland squire and Désirée. The spread of the virus PVY NTN in infected plants was monitored using different methods: DAS-ELISA, tissue printing, immuno-serological electron microscopy and real-time PCR. In all three susceptible cultivars, the virus was detected in the inoculated leaves 4-5 days after inoculation. From there virus spread rapidly, first into the stem, then more or less simultaneously to the upper leaves and roots. Real-time PCR was shown to be very sensitive and enabled viral RNA to be detected in non-inoculated leaves of susceptible cultivar Igor earlier than other methods. Therefore, for exact studies of plant-virus interaction, a combination of methods which detect viruses on the basis of their different properties (coat protein, morphology or RNA) should be used to monitor the spread of viruses. q
Physiological and Molecular Plant Pathology, 2004
The aim of this work was to correlate the appearance of the symptoms, multiplication and spread of virus after mechanical inoculation of potato (Solanum tuberosum L.) cultivars showing different levels of susceptibility and sensitivity to Potato virus Y NTN (PVY NTN). The potato cultivars used were the resistant cultivar Sante and susceptible cultivars Igor, Pentland squire and Désirée. The spread of the virus PVY NTN in infected plants was monitored using different methods: DAS-ELISA, tissue printing, immuno-serological electron microscopy and real-time PCR. In all three susceptible cultivars, the virus was detected in the inoculated leaves 4-5 days after inoculation. From there virus spread rapidly, first into the stem, then more or less simultaneously to the upper leaves and roots. Real-time PCR was shown to be very sensitive and enabled viral RNA to be detected in non-inoculated leaves of susceptible cultivar Igor earlier than other methods. Therefore, for exact studies of plant-virus interaction, a combination of methods which detect viruses on the basis of their different properties (coat protein, morphology or RNA) should be used to monitor the spread of viruses.
Cytopathological Potato virus Y structures during Solanaceous plants infection
Micron, 2012
The ultrastructural analysis of tobacco, potato and pepper tissues during infection with necrotic strains and the ordinary Potato virus Y strain of revealed the presence of virus inclusions not only in the epidermis and mesophyll but also in the vascular tissues. For the first time cytoplasmic inclusions were documented in companion cells and phloem parenchyma as well as in xylem tracheary elements. The ultrastructural features studied in this work consisted of mostly laminated inclusions (in the traverse and longitudinal section), which were frequently connected with enlarged cisternae of endoplasmic reticulum (ER) located in the direct vicinity of the cell wall attached to virus particles opposite to plasmodesmata. It was noticed that ER participates in synthesis and condensation of the PVY inclusions. During compatible interaction of tobacco and potato plants with PVY, amorphous and nuclear inclusions were observed. Such forms were not found in pepper tissues and potato revealing the hypersensitivity reaction to the infection with PVY necrotic strains. It was stated that the forms of cytoplasmic inclusions cannot serve as a cytological criterion to distinguish the potato virus Y strains and do not depend on host resistance level. Only in compatible interaction in Solanaceous plants tissues cytoplasmic inclusions were observed from the moment the morphological symptoms appeared. In the reaction of hypersensitivity, the inclusions were found on the 24th day following the infection with the PVY necrotic strains, whereas the symptoms were observed 3 days after the PVY infection.
Acta Physiologiae Plantarum, 2010
Potato plants cv. Rywal with hypersensitivity gene Ny-1 infected with PVY N or PVY NTN reacted in local necroses 3 days after infection. Potato virus Y (PVY) particles were found in epidermis, mesophyll, phloem and xylem cells in inoculated leaves. Noncapsidated virus particles (without capsid protein) were observed already 10 h after infection by using electron microscopy in situ. Capsid protein on one terminus of noncapsidated virus particles was located 5 days after inoculation with the use of immunogold labeling method. Whereas cytoplasmic inclusions were observed for the first time 24 days after infection during hypersensitive response. Ultrastructural studies showed that ER may take part in PVY RNA replication and capsidation of Potyvirus particles. Observed cytopathological changes and virus particles indicate that cell nucleus and mitochondrion might participate in PVY life cycle. During hypersensitive response PVY particles were found in plasmodesmata as well as in phloem and xylem.
MOLECULAR CHARACTERIZATION OF POTATO VIRUSES THROUGH RT-PCR AND ELECTRON-MICROSCOPY
In the present investigation viruses infecting potato were identified as Potato Virus X, Potato Leaf Roll Virus , Potato Virus S and Potato Mosaic Virus .These isolates were further characterized by Reverse Transcript-Polymerase Chain Reaction (RT-PCR) and electron-microscopy. The total RNA was extracted using spectrum Tm plant total RNA kit and their instructions. In RT-PCR diagnosis, Potato virus X (PVX) and Potato leaf roll virus (PLRV) showed amplification with their respective primers. Electron micrographs of purified virus (es) revealed flexuous (515x13nm) and isometric (24-25nm) virus particles resembling PVX and PLRV, respectively.
Novel Research in Microbiology Journal, 2019
Potato Leaf Roll Virus (PLRV) is one of the most serious viruses infecting potato plant (Solanum tuberosum L.) in Egypt. Indirect Enzyme Linked Immunosorbent Assay (Indirect-ELISA) results revealed that 70 % of the collected samples were infected with PLRV. A multiplex Polymerase Chain Reaction (PCR) was carried out using three different sets of primers, specific for both PLRV and Potato virus Y (PVY) isolates. For confirmation; the movement coat protein (MP) gene was isolated from the infected plant tissues, and a band with molecular size 336 bp was obtained using Reverse transcription-Polymerase chain reaction (RT-PCR). The DNA sequence of the Egyptian PLRV-Banha-MP gene was deposited in GenBank under an accession number of KR002119. Moreover, sequence analysis revealed that the Egyptian PLRV isolate was closely related to a New Zealand isolate of PLRV (GU002341), with identity of 100%. Transmission electron microscope (TEM) examination of PLRV showed isometric particles, with approximate size of 24-30 nm. The cytopathological examination of the potato plant infected with PLRV revealed many cellular effects such as; partially degraded and deformed chloroplast, starch with an increased size and color change, in addition to nucleus and cytoplasmic bridge. It could be concluded that PLRV is present in Egypt, infecting most of the potato cultivars. Moreover, four different strains of PLRV were detected based on the Single strand conformation polymorphism (SSCP) of the MP gene. The aims of the current study were to identify the PLRV infection of potato plant in Egypt using; molecular, serological and biological methods, in addition to studying the effect of this virus on potato cell organelles. This is the first record of the presence of four different strains of PLRV infecting potato in Egypt, using SSCP assay.
Research of necrotic and ordinary isolates of Y-virus of potato
Ustyim Experimental Plant Plant of the Institute of Plant Science named after V.Ya. Yuryeva NAAS The purpose. To determine biological characteristics of 2 isolates of Y-virus of potato, belonging to different groups of strains. One of isolates caused necrotic symptoms on plants of potato, another – of soft mosaic. Methods. Bioassays on plant-indicators. Direct methods of research of viruses: electronic microscopy withnegative contrasting, DAS-ELISA for research and identification of the revealed isolates. Results. There were revealed 2 isolates of Y-virus of potato, one of which caused striped mosaic, another – light mosaic. Necrotic isolate which caused light mosaic on host-plant – potato, caused symptoms of system necrosis on display plants of N. tabacum (cultivar Samsun). Usual isolate which induced light mosaic on plants of potato, on plant-indicators caused mosaic. Conclusions. Biological characteristics of 2 isolates of Y-virus of potato are investigated. It is determined that according to phenotypical features of infection of isolates on plant-indicators these isolates belong to different strain groups – necrotic and usual strains. The further molecular research of phylogenetic relationship of the revealed isolates is necessary. Support for agrobiodiversity is not possible without the use of a large variety of cultivar varieties. Due to the wide varieties of cultures, the need for assortment of agricultural products is met, sustainable yields are provided, and the bases of biosecurity of the state are created. An important issue is the receipt of seed material of various varieties, which must meet the requirements of high condition and be free from pathogens. Potato seed certification programs aimed at getting large seeds of seeds have faced increasing problems in the last decades in the visual detection of plants with viral infections. Detecting infections of viruses that are economically important due to their high vulnerability to potato culture is especially needed for seed certification programs. In particular, it is Y-potato virus (Potato virus Y, PVY), which has become widespread in potato culture and can reduce the yield of tubers by 30-70%. His strains can induce serious symptoms not only on the leaves of plants, but also on the tubers, causing the disease of necrotic ring spots of potato tubers. Detection of these strains is extremely important for the development of effective strategies for controlling the viral infection [1,2]. The variability of the symptoms of a viral infection can be caused both by the properties of the variety, and by the action of abiotic factors and creating obstacles to the detection of infected plants. Awareness of the fact that PVY is becoming a top-priority threat to potato seed certification programs, prompts virologists to thoroughly investigate all the factors that may lead to this. The classification of isolates PVY has historically developed on the basis of detecting the supersensitivity reaction in potato plants with the Nytbr or Nc resistance genes, as well as in the study of induction of necrosis in tobacco plants N. tabacum. From the now known five PVY strain groups, only isolates of PVYN PVYNTN groups can cause necrosis in tobacco plants [3]. In addition, new strains developed as recombinant variants of PVY (PVY N: O, PVY N-Wi and PVYNTN) are often associated with moderate symptoms on the leaves. These symptoms may be temporary in many popular potato varieties or the infection may be asymptomatic [4, 5]. The absence of symptoms on plants in breeding areas is often considered a manifestation of resistance to a virus, when in fact it may indicate asymptomatic infection in plants of tolerant varieties. These varieties sometimes contain the content of the virus, similar or exceeding this figure in varieties with clear symptoms [6]. In plants of these asymptomatic or
Physiological and Infectious Characters of Potato virus Y-Egyptian isolate
Egyptian Journal of Microbiology
P OTATO VIRUS Y (PVY) is one of the most destructive aphid transmitted pathogen to potato plants worldwide. In Egypt, PVY infection causing about 80% reduction the global yield of potato. The objective of this study was to characterize Potato virus Y (PVY-EG) infecting potato plants, based on biological, serological and molecular properties. Naturally infected potato plants by PVY gave positive reaction with PVY-polyclonal antibodies using DAS-ELISA for virus identification. PVY has a higher stability at 45 h (Longevity), 66°C at 10 min (TIP) at 10-6 (DEP), revealing presence of cytoplasmic and crystalline inclusion bodies of the epidermal strips from infected Datura metel (diagnostic host) leaves at 12 days post PVY inoculation. The PVY has UV-spectra at λ 235 and at λ 257 nm. PVY yield was 1.25/100 g leaf tissues and 260/280 more than 1 (it was 1.6). The viral particles were rod flexible (helical symmetry) of 11 x 570 nm, with obvious immunogenicity that represented by 1:1024 titer of antibodies. The cDNA fragment of CP gene was 610 bp. Sequence analysis revealed that PVY isolate showed 93-99% similarity with other worldwide PVY isolates.