Mapa físico y genético del cromosoma de Gluconacetobacter diazotrophicus PAL5 (original) (raw)

2008, Revista Latinoamericana de Microbiología

A macrorestriction map of the Gluconacetobacter diazotrophicus PAL5, an important nitrogen-fixing endophyte of several plants such as sugarcane, coffee, sweet potato and pineapple, was constructed using pulsed-field gel electrophoresis (PFGE) and DNA hybridization. For PFGE analysis, single and double digestions of the chromosome were carried out with I-CeuI, SwaI and PacI restriction enzymes. The resulting DNA fragments were positioned into physical map, using combinatory analysis of hybridization results, restriction enzymes sites position and DNA fragments length. G. diazotrophicus has 4 rrn operons into a circular chromosome, with estimated length of 4,240 Kb ± 41.6 Kb. The hybridization results allowed the positioning of 42 genetic markers on chromosome (39 single copy and 3 repeated elements), as well as the detection of 1 rrn operon with inverted orientation. The results contained in this paper were important to finish the genome assembly of the G. diazotrophicus PAL5, which was deposited and is available in the EMBL database.

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