Human Papillomavirus E6 biosensing: Current progression on early detection strategies for cervical Cancer (original) (raw)
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Research Square (Research Square), 2022
Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. In this study, we developed an electrochemical DNA biosensor for detection of HPV-16 and HPV-18, which are the 2 most prevalent genotypes for development of oral and cervical cancers. The assay involves the sandwich hybridization of the HPV target to silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly speci c and sensitive, with detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM to 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR /gel electrophoresis detection. The biosensor assay could be completed within 90 minutes. Due to its simplicity, rapidity and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories.
Advancements in electrochemical DNA sensor for detection of human papilloma virus - A review
Analytical biochemistry, 2018
Human papillomavirus (HPV) is one of the most common sexually transmitted disease, transmitted through intimate skin contact or mucosal membrane. The HPV virus consists of a double-stranded circular DNA and the role of HPV virus in cervical cancer has been studied extensively. Thus it is critical to develop rapid identification method for early detection of the virus. A portable biosensing device could give rapid and reliable results for the identification and quantitative determination of the virus. The fabrication of electrochemical biosensors is one of the current techniques utilized to achieve this aim. In such electrochemical biosensors, a single-strand DNA is immobilized onto an electrically conducting surface and the changes in electrical parameters due to the hybridization on the electrode surface are measured. This review covers the recent developments in electrochemical DNA biosensors for the detection of HPV virus. Due to the several advantages of electrochemical DNA bios...
Electrochemical DNA biosensor for human papillomavirus 16 detection in real samples
Analytica Chimica Acta, 2013
h i g h l i g h t s • Electrochemical biosensor was used to detecting human papillomavirus (HPV) in real samples from endocervical swabs. • The detection limit was of 18.13 nM. • No hybridization with noncomplementary sequence showed that the method is selective. • It can be an excellent approach to detect human papillomavirus (HPV) in real samples.
Voltammetric DNA Biosensor for Human Papillomavirus (HPV) Strain 18 Detection
IOP Conference Series: Materials Science and Engineering, 2020
This research was developed to focus on the study of the voltammetric DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor was expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection was conducted to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of the DNA probe. In this project, synthetic DNA sample and DNA from the saliva of several Biosystems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrodes on the IDE was detected under SEM. Surface modification of th...
Biosensors
Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. Hence, we developed a multianalyte electrochemical DNA biosensor that could be used for both oral and cervical samples to detect the high-risk HPV genotypes 16 and 18. The assay involves the sandwich hybridization of the HPV target to the silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly specific and sensitive, with a detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM and 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR/gel electrophoresis detection. The biosensor assay could be completed within 90 min. Due to its simplicity, rapidity, and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories as well as for epidemiological studies.
Clinical Oncology and Research, 2021
Cervical cancer has the highest mortality rate worldwide. In the quest for reducing such a high mortality rate, advancements in diagnosis as well as treatment are being undertaken at various scales across the globe. With the recent advancements in the applications of nanotechnology, simple, rapid and inexpensive diagnostic methods for cervical cancer, i.e., human papillomavirus (HPV), especially high-risk oncogenic subtypes 16 and 18 have started to gain attention of health care practitioners. This review outlines the current applications of biosensors for the diagnosis of HPV, as compared to the conventional techniques for measuring HPV that have some limitations. The traditional methods used for cervix cancer are less sensitive, whereas nanotechnology has greatly improved the sensitivity. Due to cancer incidence and mortality growing rapidly worldwide, the prevalence and risk factors are also discussed in this review.
Journal of Nanobiotechnology
Background In several years ago, infection with human papillomaviruses (HPVs), have been prevalent in the worlds especially HPV type 18, can lead to cervical cancer. Therefore, rapid, accurate, and early diagnosis of HPV for successful treatment is essential. The present study describes the development of a selective and sensitive electrochemical biosensor base on DNA, for early detection of HPV-18. For this purpose, a nanocomposite of reduced graphene oxide (rGO) and multiwalled carbon nanotubes (MWCNTs) were electrodeposited on a screen-printed carbon electrode (SPCE). Then, Au nanoparticles (AuNPs) were dropped on a modified SPCE. Subsequently, single strand DNA (ssDNA) probe was immobilized on the modified electrode. The link attached between AuNPs and probe ssDNA provided by l-cysteine via functionalizing AuNPs (Cys-AuNPs). The differential pulse voltammetry (DPV) assay was also used to electrochemical measurement. The measurement was based on the oxidation signals of anthraqun...
BMC infectious diseases, 2003
We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor) in identifying multiple pathogens. Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes. Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples. This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.