Inhibitory Effect of the Intrauterine Infusion of Phorbol 12-Myristate 13-Acetate and 1-Oleoyl-2-Acetylglycerol on the Decidual Cell Reaction in Rats1 (original) (raw)
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Reproduction, 1983
Intrauterine instillation of oil, but not saline, induced both a decidual cell reaction and a marked elevation in the uterine PGF-\g=a\content of suitably sensitized ovariectomized mice. Uterine PGF-\g=a\concentrations were elevated within 5 min of the oil instillation, reached maximal levels within 30\p=n-\60min and then declined to near baseline levels again by 3 h. A similar increase in uterine PGF-\g=a\content in response to oil instillation was seen in non-sensitized females, although no decidual cell reaction developed. No significant changes in PGE or 6-oxo-PGF-1 content were observed. These results suggest that although the increase in uterine PGF-\g=a\ content is not solely due to the distension of the uterus after intrauterine injection, the increase is not necessarily sufficient to induce a decidual cell reaction.
Prostaglandin F2α and Its Receptor as Activators of Human Decidua
Seminars in Reproductive Medicine, 2007
Prostaglandins produced by the intrauterine tissues of both mother and fetus (myometrium, decidua, placenta, chorion, and amnion) are involved with all of the physiologies of parturition (membrane rupture, cervical dilatation, myometrial contractility, placental separation, and uterine involution). For parturition to occur, however, the intrauterine tissues need to first be activated to prepare for the work of labor, then stimulated to initiate labor. Prostaglandins normally are considered to be stimulators of the physiologies of labor. This review presents evidence that one prostaglandin, PGF 2a , and its receptor, FP, are also activators, especially of the decidua. Stimulated by cytokines, the decidual synthesis of PGF 2a and the expression of FP lead to increased matrix metalloproteinase activity, further enhancement of cytokine activity, increased decidual oxytocin and oxytocin receptor expression, decreased progesterone responsiveness, and possibly, enhanced expression of vascular endothelial growth factor. These collective actions prepare the decidua for its role in parturition.
Effect of deciduogenic stimuli on protein secretion by the mouse uterus
Reproduction, 1988
Instillation of oil into progesterone-primed, oestrogen-sensitized uteri of mice resulted in secretion patterns which were similar, but not identical, to those found on Day 5 of pregnancy. Stimulus-dependent responses common to pregnancy and the experimental decidual cell reaction included an early but transient increase in a 40 000 Mr basic protein and decreases in two other proteins. Some of the characteristic changes were also found after oil instillation in the progesterone-maintained 'non-receptive' uterus, even though subsequent decidualization did not occur. Instillation of cholera toxin, another deciduogenic substance, also resulted in patterns similar to those of pregnancy, including an increased secretion of a 25 000 Mr acidic protein which was only minimally produced during the oil-induced decidual cell reaction.
Metabolic factors regulating the generation of prostaglandins E1 and E2 by isolated rat uterus
Prostaglandins, Leukotrienes and Medicine, 1983
The generation and output of PEE2 and PGE, by isolated rat uterus incubated with or without glucose as well as the influence of different metabolic substrates and selective enzyme inhibitors on tissue formation and release of PGE material of the 1 and 2 series, were explored. The output of PEE2 was comparable with glucose, fructose, lactate or pyruvate as the substrate but diminished significantly in citrate-containing solution. In aub-&rate-free media uteri released more PGE than in the presence of glucose or other substrates. The PGEl z iberated was similar in glucose, fructose, lactate or pyruvate and decreased significantly in citrate or without substrate. 2-deoxy-D-glucose (2-DG), fluoride or arsenite failed to alter significantly the release of PEE2 whereas 2,4_dinitrophenol (ONP> evoked augmentation. The uterus released less PEEI after 2-DE or fluoride but not following the addition of DNP or arsenite. The results suggest that the synthesis and release of PGE2 and PGE, in the isolated rat uterus appear to be selectively modulated by different tissue metabolic pathways.
Reproduction, 2009
To determine if changes in endometrial expression of the enzymes and receptors involved in prostaglandin (PG) synthesis and action might provide insights into the PGs involved in the initiation of decidualization, ovariectomized steroid-treated rats at the equivalent of day 5 of pseudopregnancy were given a deciduogenic stimulus and killed at various times up to 32 h thereafter. The expression of PG-endoperoxide synthases (PTGS1 and PTGS2), microsomal PGE synthases (PTGES and PTGES2), cytosolic PGE synthase (PTGES3), prostacyclin synthase (PTGIS), prostacyclin receptor, peroxisome proliferator-activated receptor δ (PPARD) and retinoid x receptor α (RXRA) in endometrium was assessed by semiquantitative RT-PCR, western blot analyses and immunohistochemistry. In addition, to determine which PG is involved in mediating decidualization, we compared the ability of PGE2, stable analogues of PGI2, L165041 (an agonist of PPARD), and docasahexanoic acid (an agonist of RXRA) to increase endome...
Journal of the …, 2006
OBJECTIVE: Prostaglandins (PGs) are key regulators of cervical dilatation and membrane breakdown at the onset of labor. PG synthase and receptor expression has been previously documented in uterine tissues; however, mechanisms governing the changes occurring in the cervix and amnion are less well established. The aim of the current study was to determine the level of expression of PG synthetic enzymes and receptors in these tissues in association with induced labor in sheep. METHODS: Labor was induced in sheep at 135 days of gestation by continuous fetal dexamethasone infusion. Amnion and cervical tissue was obtained before and after laborfor measurement ofmRNA encoding enzymes (cytosolicphospholipase A2 [cPLA2], PGH synthase-2 [PGHS-2], PGFsynthase [PGFS], and PGE synthase [PGES]) and receptors (FP and EP1-4) by real-time polymerase chain reaction (PCR). RESULTS: cPLA2 expression increased significantly in cervical tissue at labor onset, whereas expression of the other enzymes measured did not change. There was a marked rise in EP3 expression in the cervix, but abundance of this receptor was lower than EP2 and FP expression, which did not change. The amnion exhibited a labor-associated decrease in PGHS-2, PGFS, and FP mRNA expression. CONCLUSION: The regulation of PG synthesis and action occurring in the amnion and cervix in association with labor appear to difer markedly between the two tissues, indicating tissue-specfic rolesfor PGs. The data support a rolefor increased PG synthesis and action in the cervix and suggest a decrease in PG production and action in the amnion, in sharp contrast to the pattern reported in human amnion. Soc