Isolation and Biochemical Characterization of Endophytic Bacterium Gluconacetobacter diazotrophocus from Native Sugarcane Cultivar of middle Gangetic Plains of India (original) (raw)

2024, Isolation and Biochemical Characterization of Endophytic Bacterium Gluconacetobacter diazotrophocus from Native Sugarcane Cultivar of middle Gangetic Plains of India

https://doi.org/10.55362/IJE/2024/4202

The study focused on the isolation and characterization of Gluconacetobacter diazotrophocus, an endophytic bacterium obtained from various parts of a native sugarcane cultivar in India's middle Gangetic plains. Using conventional culturing methods, isolated and screened 115 isolates, ultimately selecting 15 highly efficient isolates based on their production of growth-promoting hormones. Among these, GdS08S, isolated from sugarcane cultivar CoP-9301, exhibited the highest nitrogen-fixing ability, with 153μg of N/mg of carbon used. Isolate GdS15S, from sugarcane cultivar Co-0238, produced the highest concentration of gibberellic acid (8.19μg/25ml). Additionally, GdS07R displayed notable phosphorus and zinc solubilization zones, measuring 4.22 cm and 4.52 cm, respectively. Gluconacetobacter diazotrophocus, residing within sugarcane tissues, plays a pivotal role in promoting rooting, cell elongation, and overall sugarcane growth through the biosynthesis of indole-3-acetic acid (IAA) and gibberellic acid (GA). Furthermore, it actively fixes atmospheric nitrogen, offering a significant nitrogen supply to the growing crops. This research underscores the significance of G. diazotrophocus as a potential biofertilizer for native sugarcane cultivars in the middle Gangetic plains of India, contributing to sustainable agriculture practices.