Production of Cellulase and Amylase Enzymes in both Solid and Liquid States by Two Species of Fungi (original) (raw)

2022, Asian Journal of Biotechnology and Bioresource Technology

Aims: To isolate fungi capable of simultaneous production of amylase and cellulase Study Design: The experiment was carried out in aseptic conditions, data were subjected to one way Analysis of Variance (ANOVA) and the means were separated using Least Significance Deference (LSD). Place and Duration of Study: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria, between August 2010 and July 2013. Methodology: Plantain peels and infected bark of the stump of a tree were collected for fungi isolation. A 1 g sample each was weighed separately and added to 10 ml distilled water, 1 ml of each of the diluents was plated out on Emerson’s yeast phosphate soluble starch (YPSs) medium. After growth, colonies were picked and subcultured several times for purity. The isolates were characterized and identified based on colony morphology and microscopic examination. They were later screened for amylase and cellulase activities by growing them on various concentrations ...

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