Isolation and identification of Mycoplasma agalactiae associated with respiratory tract infection in goats: A case study (original) (raw)
Related papers
Isolation of Mycoplasma species from respiratory infections of sheep and goats
The present study is carried out on isolation of Mycoplasma species from respiratory infections of sheep and Goats because Mycoplasma is also one of the major etiological agent in the production of respiratory infections which produces economic losses to small-scale farmers. In the present study a total of 188 Mycoplasma suspected samples, 95 (44 nasal swabs, 49 lung tissues and 2 pleural fluids) from sheep and 93 (56 nasal swabs, 33 lung tissues and 4 pleural fluids) from goats were collected. Isolation carried out on PPLO broth and PPLO agar. Positive samples produce color change in broth from brown to yellow and production of two different types of colonies on agar. One type is typical fried egg colony and other type is nipple-like with prominent tip. Conformation also done by PCR targeting Mycoplasma genus yielding 280 bp product size.
Investigation of Mycoplasma agalactiae in sheep in Kurdistan province by PCR
archives of razi institute, 2015
Contagious agalactia (CA) is a serious disease syndrome of sheep and goats that is characterized by mastitis, arthritis, keratoconjunctivitis and, occasionally, abortion. Mycoplasma (M.) agalactiae is the main cause of the disease in sheep and goats. The aim of the present study was to detect M. agalactiae in conjunctival, synovial fluid, nasal, ear and milk samples in sheep herds with or without CA sign in Kurdistan province. One hundred and seventy three samples analyzed were taken from sheep herds reared in a CA endemic area. Of the samples tested, 130 were positive by PCR for Mycoplasma spp. and of these, 19 showed a positive result for M. agalactiae . M. agalactiae was detected in conjunctival (7/59), synovial fluid (1/8) and milk (11/21) samples. Ear and nasal swap samples were free of M. agalactiae . The results of 60 samples in sheep without signs of CA syndrome showed that Mycoplasma spp. and M. agalactiae were detected in 25 and 6 samples, respectively. Our findings indica...
2014
Background: Contagious agalactia (C.A) is an infectious syndrome of sheep and goat characterized by mastitis, arthritis, keratoconjunctivitis and abortion. Mycoplasma agalectiae is the main cause of the disease in small ruminants. Objective: The aim of this study was to isolate and identify Mycoplasma mycoides cluster using PCR and culture from goats of Kerman city in Iran. Results: A total of 298 samples were collected from ear, eye and nose swab and milk secretion. All samples were cultured in PPLO broth and agar. After culture 56 samples (18.79%) were positive and showed fried egg colony on the agar media. At the same time the bacterial DNAs were extracted by Phenol-Chloroform method and the PCR assay was applied for detection of Mycoplasma genus in 163bp fragment of 16SrRNA gene, M.agalectiae in 375bp fragment of lipoprotein gene and M.mycoides cluster in 259bp fragment of 16SrRNA gene in all clinical samples. Out of 298 samples, 127 samples (42.6%) were positive for Mycoplasma-...
Journal of Veterinary and Animal Sciences, 2021
Mycoplasmal pneumonia is an important contagious disease that significantly affects the economy of small ruminant farming worldwide and Mycoplasma ovipneumoniae (M. ovipneumoniae) is one of the major aetiological agents associated with pleuropneumonia in goats. It is considered as a serious epidemic disease of goats due to its huge economic impact and hence, rapid and early diagnosis of the disease is warranted. Clinical mycoplasmosis often lacks pathognomonic signs, so definitive diagnosis of the disease is quite burdensome. Polymerase chain reaction (PCR) test has been proven to be a specific and sensitive technique for the early diagnosis of mycoplasmosis. The present study highlights the detection of M. ovipneumoniae employing PCR test in 150 nasal swab samples collected from goats with symptoms of respiratory tract infection from five districts of Kerala. Results revealed that, out of 150 samples, 83 (55.33 per cent) were positive in 16S rRNA Mycoplasma genus specific PCR test....
Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds
Acta Veterinaria Brno, 2007
The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan. Mycoplasma agalactiae, sheep, goats, Jordan, polymearase chain reaction Contagious agalactia of sheep and goats is a serious infectious disease affecting small ruminants. It is characterised by arthritis, keratoconjunctivitis, pneumonia and, in females, mastitis and occasional abortion. It may be caused by any of the five mycoplasma species associated with this disease, namely, Mycoplasma agalactiae, Mycoplasma mycoides subsp. mycoides LC (large colony), Mycoplasma capricolum subsp. capricolum, Mycoplasma mycoides subsp. capri and Mycoplasma putrefaciens (Nicholas 1996; Sarris 1996; Bergonier et al. 1997; Bölske 1994). However, Mycoplasma agalactiae (M. agalactiae) is still regarded, particularly in sheep, as the "classical" aetiological agent of contagious agalactia (Bergonier et al. 1997). Mycoplasma putrefaciens is found only in goats. However, other animal species, such as cattle, camels or small wild ruminants, can also be infected (Perrin et al. 1994). Contagious agalactia is at present an important disease in countries with intensive small animal husbandry, such as the Mediterranean region and the Balkan Peninsula, Western
Goats were infected experimentally with a mycoplasma (the ''Irbid'' strain) isolated previously from a goat with contagious agalactia in northern Jordan. The strain was unusual in that, although it had been identified by molecular methods as Mycoplasma mycoides subsp. mycoides LC/Mycoplasma mycoides subsp. capri, it showed no inhibition of growth by any of the hyperimmune rabbit antisera conventionally used to speciate members of the Mycoplasma mycoides cluster. Animals were infected either intratracheally or by aerosol and placed ''in-contact'' with other goats. After 2 weeks, those infected intratracheally became febrile, showing a nasal discharge and slight conjunctivitis, followed a week later by respiratory distress and polyarthritis; lesions seen at necropsy included coagulative necrotic pneumonia, fibrinous pleurisy with pleural exudate, and inflammatory exudates, necrosis and fibrosis in the joints. Animals infected by aerosol showed much milder clinical signs, including nasal discharge and occasional swollen joints. In the ''in-contact'' goats, seroconversion was first seen after 7 weeks, accompanied by coughing and laboured respiration; lesions in this group consisted of fibrinous pneumonia with focal areas of necrosis and abundant pleural exudate.
2017
Contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. The disease is rapidly spread by the contact of the infected animals with the healthy ones. Domestic sheep and goats of both sexes can be infected at an equivalent frequency. Most of the researchers use culture and molecular methods for the isolation and identification of Mycoplasma. Mycoplasma agalactiae is the main cause of the disease in sheep. The aim of this study was to isolate and identify M. agalactiae by using culture and polymerase chain reaction (PCR) assay in the sheep herds in Guilan province, Iran. A total of 71 specimens were collected from seven sheep herds with clinical signs of agalactia disease. All of the seven sheep herds (100%) were positive either in PPLO agar or Mycoplasma PCR test. Out of the 71 specimens, 50 (70.4%) cases were positive; however, 21 (29.6%) samples were negative. Furthermo...
Pakistan Veterinary Journal, 2011
In this study, the presence of Mycoplasma ovipneumoniae and other mycoplasmas was investigated by culture and PCR in the nasal swab samples of 692 goats with nasal discharges in 44 different flocks in eastern Turkey. The results showed that approximately 41% (18/44) of the flocks and 11% (75/692) of the goats harbored Mycoplasma agents. Among the Mycoplasma species; M. ovipneumoniae was the most prevalent species detected within 29.5% (13/44) of the flocks and 8.1% (56/692) of the goats. Mycoplasma arginini was also detected solely or in combination with M. ovipneumoniae in 18.2% (8/44) of the flocks and 1.9% (13/692) of the goats with nasal discharges. None of the samples were determined to be positive in the M. mycoides cluster and Mycoplasma agalactiae specific PCR analyses. The remaining 12 samples could not be identified at species level. Because the investigation of other agents which may cause respiratory problems such as Mannheimia was beyond the scope of this study, it is n...
Ten goats were experimentally infected with a Mycoplasma identified by biomolecular methods as Mycoplasma mycoides subsp. capri, strain Irbid which was isolated from goats in an outbreak of contagious agalactia in north Jordan and defined as ‘unusual’, due to its serological characteristics. Two groups of goats infected by the endotracheal route and by aerosol, respectively, were placed in contact with a third group of naive animals. Six weeks after infection, some animals from both the infected and contact groups presented fever and nasal discharge, followed by severe respiratory signs and polyarthritis. Organs were taken from animals that died during the trial or those that were sacrificed at the end of the trial. The results of microbiological isolation and immunohistochemical tests conducted on the organs were compared after a description of the clinical picture and anatomopathological and histopathological signs.
African Journal of Microbiology Research, 2013
Mycoplasma agalactiae is the etiological agent of contagious agalactia, which is the first isolated mycoplasma from sheep and goats. The bacteria have been detected using polymerase chain reaction (PCR) method in Iranian goats, but there has been no investigation of the molecular characterization of contagious agalactia agents in goats in Iran. Several studies have also characterized M. agalactiae strains. The aim of this study was to analyze the DNA sequence of M. agalactiae isolates from Iranian goats, and compare it with the other Iranian isolates and vaccinal strains obtained from GenBank. Therefore, PCR with a mycoplasmal cytoplasmic membrane protein 80 KDa (P80) gene was applied to Iranian isolates and the PCR product was purified and sequenced. After the computer alignment of these gene sequences was done, the next process was comparing the result with the specific species of M. agalactiae deposited in GenBank. However, a sequence identity matrix is represented (Bio Edit, Clustal W) and a phylogenetic tree is built by the neighbor-joining method. 1000 bootstrap simulations were given at the tree's branching points and represent these points' phylogenetic relationships. Sequence analysis of different M. agalactiae showed significant variability among the field isolates. In comparison with vaccine strains, which are used in Iranian goat herds, characterized isolates under this study has a poor homology.