Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of Henna ( Lawsonia inermis ) flowers (original) (raw)
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Phytochemical Screening and Anti-inflammatory Properties of Henna Leaves (Lawsonia inermis)
European Journal of Medicinal Plants
In traditional West African setting, plants are commonly used for cosmetic and medicinal benefits such as antibacterial, antifungal, anti-inflammatory, antioxidant and antiparasitic effect of which Henna (Lawsonia inermis), a natural occurring plant of the lythraceae family is not an exception. A preliminary qualitative phytochemical screening and anti-inflammatory properties of Lawsonia inermis leaves extracted with N-butanol and ethyl acetate was investigated. Standard analytical procedures were observed, and statistical analysis using ANOVA version 20.0 was conducted to analyze for mean ± standard deviation (SD) and the least significant difference (LSD) obtained from the study. Results revealed the presence of flavonoids, tannins, terpenes, cyanogenic and cardiac glycosides; while In vivo anti-inflammatory activity wasobserved on a week old cockerel chicks induced carrageenan inflammation post extract administration, using aspirin tablet as control. The anti-inflammatory activit...
Antioxidant and Immunomodulatory Constituents of Henna Leaves
2000
The immunomodulatory bioassay-guided fractionation of the methanolic extract of henna (Lawsonia inermis L.; syn. Lawsonia alba L.) leaves resulted in the isolation of seven compounds; three have been isolated for the first time from the genus, namely p-coumaric acid, 2-methoxy-3-methyl-1,4-naphthoquinone and apiin, along with the previously isolated compounds: lawsone, apigenin, luteolin, and cosmosiin. Structural elucidation of the isolated compounds was based upon their physical, chemical as well as spectroscopic characters. Their immuomodulatory profile was studied using an in vitro immunoassay, the lymphocyte transformation assay. The ABTS [2,2Ј-azino-bis (3-ethyl benzthiazoline-6-sulfonic acid)], free radical scavenging assay depicted that all isolated compounds exhibited antioxidant activity comparable to that of ascorbic acid.
İJFER
The henna plant (Lawsonia inermis) which is known and used as a dye plant from past to present, plays a role in our lives with its cultural effects. The cosmetic feature of this plant is the lawson molecule, a red-orange pigment, which is the main coloring agent found in its leaves, and this molecule is a subgroup of naphthoquinones. Although it comes to the fore with its cosmetic feature, it is a medicinal product that has positive effects on health with its anticarcinogenic, antimicrobial, anti-inflammatory, analgesic, and antipyretic properties thanks to its bioactive compounds such as flavonoids, naphthoquinones, quinoids, naphthalene derivatives, triterpenoids, organic acids, tannins, phenolic and phenolic glycosides. determined to be a plant. This review was mainly written to give information about the phenolic, antioxidant and antimicrobial studies on the henna plant and the coloring agent Lawson molecule obtained from it.
Bioactive flavonoids from the leaves of Lawsonia alba (Henna
Phytochemistry Letters
Three new flavonoids, lawsochrysin (1), lawsochrysinin (2), and lawsonaringenin were isolated from the leaves of Lawsonia alba Lam. along with four flavonoids 3 0 ,4 0 -dimethoxy flavone (4), 7-hydroxy flavone (5), 3,3 0 ,4 0 ,7-tetrahydroxy flavanone (6) and rhoifolin hitherto unreported from this genus. Their structures were elucidated on the basis of spectroscopic evidences. Compounds and showed a good urease inhibition activity IC 50 = 139.1 AE 0.77 and 184.53 AE 0.61 mM respectively, (6) also showed a DPPH radical scavenging activity with IC 50 = 33.83 mM.
Physiology and Pharmacology
Introduction: Continuous exposure of oxidants to the skin may disrupt the antioxidant balance and leads to inflammatory skin diseases (ISD). The aim of the present study was to compare the antioxidant activity, phenolic and flavonoid content of two traditionally used plants in ISD, Lawsonia inermis and Haplophyllum vermiculare. Methods: The hydroethanolic extract of the plants was prepared by maceration. Phenolic and flavonoid content of the extracts was measured respectively with Folin-Ciocateu and aluminum chloride methods. The monovalent reducing power and radical scavenging activity were also evaluated respectively by ferric reducing antioxidant power and 2,2-diphenyl-1picryl-hydrazyl methods. Results: The reducing power of Lawsonia inermis (862.89±32.23 μmolFe 2+ /g) was significantly higher than Haplophyllum vermiculare extract (765.52±29.39 μmolFe 2+ /g). The radical scavenging activity of Lawsonia inermis extract at a concentration of 1000µg/ml (%65.72±0.77) was also significantly higher than Haplophyllum vermiculare (%36.34±2.52). The higher antioxidant activity of Lawsonia inermis is probably due to its higher phenolic (96.76±3.34μg GAE/mg) and flavonoid content (197.69±5.76μg QE/mg). Conclusion: Henna leaves had higher antioxidant activity, phenolic and flavonoid content compared to aerial parts of Haplophyllum vermiculare, and may be more effective in improving oxidative stress, prevention and treatment of ISD.
The analgesic and anti-inflammatory effect of lawsone isolated from Lawsonia inermis
ScienceRise: Pharmaceutical Science, 2022
This study aimed to assess the analgesic and anti-inflammatory effects of lawsone isolated from henna leaves (Lawsonia inermis). Methods: A total of 120 healthy adult mice (weighing about 25±5 g) were included in this study. Sixty mice out of the total were used to determine LD50, 30 mice to determine the anti-inflammatory test, and the remaining (30 mice) were used for an analgesic test. The hot plate was used to determine the analgesic effect, while the anti-inflammatory effect was determined by the ability of the compound to minimize the inflammation and edema caused by the injection of carrageenan. Results: Lawsone was isolated from Lawsonia inermis. A Stuart SMP10 digital melting point apparatus was used for measuring all melting points. Infrared spectrometer FT-IR 400D was used for measuring/recording IR spectra (KBr) which the frequency of absorption was represented as cm−1. For 1H-NMR spectrum recording, a Bruk-spectrophotometer of 400 MHz was used with internal TMS stand...
Lawsonia Inermis Linn: A Plant with Cosmetic and Medical Benefits
International Journal of Applied Sciences and Biotechnology, 2016
Plants play a major role on the earth and human beings depend on plants because of their medicinal properties. About 80% of the world population using plants as a medicinal drugs because plants have no side effects and show synergistic effect unlike modern medicine. In the present study, review and authentication of the various aspects of the plant Lawsonia inermis was carried out. This plant is mainly present in subtropical and tropical areas and is used in all over the world. The common name of L. inermis is Henna and Mehndi. It has been used for over 9000 years for their cosmetic values as a dye. Traditionally, in Asian countries like India and Pakistan, plant leaves are applied to hands, hairs and feet. Morphologically the plant is a small tree or shrub. L. inermis is cultivated for roots, flowers, stem bark and seeds for their medicinal uses. Proteins, carbohydrates and fatty acid are primary metabolites along with secondary metabolite such as tannins, quinines, terpenoids, coumarins etc which are present in L. inermis plant. Phytochemical constituents of L. inermis are responsible for its analgesic, antiinflammatory, hepatoprotective and hypoglycaemic properties. The plant has also been reported for antibacterial, antifungal, immunostimulatory, antioxidant and cytotoxic activity. Presence of these properties in L. inermis plant develops it as medicine against various pathogenic organism and diseases. This review gives a wide view on the phytochemistry, pharmacological properties and traditional uses of the plant.
Plant science today, 2024
Lawsonia inermis L., commonly known as Henna, is a plant that has been extensively utilized throughout the ages for its medicinal and cosmetic properties. This plant contains a variety of bioactive compounds and has attracted significant interest due to its potential antioxidant and antimicrobial activities. The aim of our study is to evaluate the antioxidant capacity and antibacterial activity of Lawsonia inermis seeds. L. inermis seed powder was extracted using hexane, dichloromethane, ethanol and water. The 4 fractions were quantified for polyphenols, flavonoids and tannins contents. The antioxidant capacity was assessed using 4 methods: 2,2′-Diphenylpicrylhydrazyl hydrate (DPPH) , 2,2'-azino-bis(3-ethylbenzothiazoline-6sulfonic) (ABTS), Ferric Reducing Antioxidant Power (FRAP), and Phosphomolybdate assay (PMA). Antimicrobial activity was determined by agar diffusion against Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The liquid dilution method was used to determine the minimum inhibitory concentration, minimum bactericidal concentration and minimum fungicidal concentration. The highest content of polyphenols and tannins was obtained in the ethanolic fraction, with values of 594.01±6.07 mg GAE/g dw and 242.4±1.6 mg CE/g dw respectively. The ethanolic fraction exhibited significant scavenging of free radicals (DPPH) with an IC50 of 3.06±0.01 µg/mL, lower than the IC50 of the standard quercetin (5.30±0.02 µg/mL). Moreover, the ethanolic fraction showed antimicrobial activity against S. aureus, B. cereus and E. faecalis strains, with zone of inhibition values of 15.5±0.7, 14.0±0.0 and 14.5±0.7 mm respectively. The minimum inhibitory concentration of the ethanolic fraction was found to be 1.563 mg/mL against both S. aureus and B. cereus strains, while the minimum bactericidal concentration was 3.125 mg/mL against B. cereus and 6.25 mg/mL against E. faecalis strains. L. inermis seeds exhibit significant antioxidant capacity and demonstrate antimicrobial activity. These findings indicate the potential of L. inermis seeds as a valuable source of bioactive compounds with antioxidant and antimicrobial properties.