Chemical Composition and Antimicrobial and Antioxidant Activities of Essential Oils and Various Extracts of Juniperus phoenicea L. (Cupressacees) (original) (raw)
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Mediterranean Journal of Chemistry
This study aimed to compare the chemical composition, the antioxidant activity and the antibacterial activity of essential oils (EOs) of Juniperus phoenicea, Juniperus thurifera and Juniperus oxycedrus, obtained from Ait Bouguemez region (Province of Azilal, Morocco). The analysis by GC/MS of essential oils led to identify 37, 54 and 38 components for J. phoenicea, J. thurifera, and J. oxycedrus, respectively. Monoterpenic fraction was found predominant in essential oils of the three samples. The DPPH free radical scavenging activity showed that essential oil of J. thurifera has the strongest antioxidant activity with an IC50 value of 12.07 μg/mL. The antibacterial activity showed that S. aureus was more sensitive than P. aeruginosa and E. coli for the three EOs tested.
Antimicrobial Activity and Chemical Analysis of the Essential Oil of Algerian Juniperus phoenicea
Natural product communications, 2016
The essential oils of Juniperus phoenicea L. from Algeria were obtained by hydrodistillation and analyzed by GC-FID and GC-MS. Concerning their chemical composition, 74, 61 and 72 volatile compounds were identified from fresh leaves, dried leaves and berries, representing 88.8%, 91.3% and 94.7% of the total composition, respectively. The main monoterpene in the oils of fresh leaves, dried leaves and berries was a-pinene (29.6% / 55.9% / 56.6%), accompanied by lesser amounts of the sesquiterpenes β-caryophyllene (2.6% / 1.6% /1.2%) and germacrene D (2.01% / 1.7% / 1.5%), respectively. Antibacterial activity of J. phoenicea essential oils was tested against one Gram-negative and four Gram-positive bacterial strains and the yeast Candida albicans, responsible for nosocomial infections. As references, 14 antibiotics and 5 antifungal agents were evaluated. The berry essential oil was ineffective against all but two of the strains tested, whereas the essential oil of dried leaves signific...
Chemical Composition and Antibacterial Activity of the Essential Oil of Moroccan Juniperus phoenicea
Natural Product Communications
The antibacterial activity of the essential oil from leaves of Juniperus phoenicea (Cupressaceae) and its chemical constituents were investigated in this study. The essential oil was obtained by hydro-distillation and analyzed by gas chromatography equipped with a flame ionisation detector (GC-FID), and gas chromatography coupled to a mass spectrometer (GC-MS). Sixty-three volatile compounds were identified representing 52 to 92% of the total oil compositions. The main monoterpenes were α-pinene (26.7-78.7%) and δ-3carene (7.6-15.4%). The antibacterial activity of J. phoenicea essential oil, when tested against both Gram-positive and Gram-negative bacteria, showed high activity against all bacteria tested, except Pseudomonas.
Open Chemistry
Juniperus phoenicea (L.) is a medicinal plant that has been used in phytotherapy as a treatment of certain pathological infections. In this context, the present work aimed to valorize the essential oil of J. phoenicea seeds (EOGP) by studying its chemical composition, and antioxidant and antimicrobial activities. The EOGP was extracted by use of hydrodistillation and characterized by gas chromatography (GC–MS). The antioxidant power was evaluated by three methods (TAC, DPPH, and FRAP). The antimicrobial power was evaluated against Staphylococcus aureus (ATCC6633), Escherichia coli (K12), Bacillus subtilis (DSM6333), Proteus mirabilis (ATCC29906), Candida albicans (ATCC10231), Aspergillus niger (MTCC282), Aspergillus flavus (MTCC9606), and Fusarium oxysporum (MTCC9913). The GC/MS results revealed a total identification of 99.98% with a dominance of carvacrol (39.81%) followed by p-cymen-3-ol (34.44%) and o-cymene (13.60%). Findings showed that EOGP exhibited important antioxidant pow...
Background: There are no information of the yield, chemical composition and antimicrobial activity of essential oils of berries (EOB) or leaves (EOL) of Juniperus excelsa Bieb. (Cupressaceae) growing wild in R. Macedonia. Materials and Methods: Plant material was collected from two localities during two seasons. Essential oil composition was analyzed by gas chromatography/flame ionization detector/mass spectrometry (GC/FID/MS) and antimicrobial screening was made by disc diffusion and broth dilution method. Results and Discussion: EOB yield ranged from 1.6-9.4 ml/kg and from 8.9-13.9 ml/kg for EOL. Two chemotypes of essential oil were differentiated, α‑pinene‑type (with 70.81% α‑pinene in EOB and 33.83% in EOL), also containing limonene, β‑pinene and β‑myrcene while the sabinene‑type (with 58.85-62.58% sabinene in EOB and 28.52-29.49% in EOL), was rich in α‑pinene, β‑myrcene, limonene, cis‑thujone, terpinolene and α‑thujene. The most sensitive bacteria to the antimicrobial activity of EOB was Haemophilus influenzae (MIC = 31 µl/ml). EOL have showed high activity towards: Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae (MIC = 125 µl/ml). The pinene‑type of essential oil showed moderate activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp. and Campylobacter jejuni (MIC >50%). The sabinene‑type of the oil showed moderate activity to Streptococcus pyogenes, Haemopilus influenzae, Campylobacter jejuni and Escherichia coli (MIC >50%). No activity was observed toward Candida albicans. Conclusion: The analysis of EOB and EOL revealed two chemotypes (α‑pinene and sabinene type) clearly depended on the geographical origin of the Macedonian Juniperus excelsa which also affected the antimicrobial activity of these oils. Key words: Antimicrobial activity, essential oil, gas chromatography/flame ionization detector/mass spectrometry analysis, juniper berries and leaves, Juniperus excelsa
Acta Scientiarum Polonorum-hortorum Cultus, 2023
Juniper species are especially used in traditional medicine due to their analgesic, diuretic, antibacterial, antimicrobial, anti-inflammatory, and liver-protective effects. The goal of the present study was to investigate the chemical compounds, antioxidant and antimicrobial properties of essential oil of Juniperus excelsa M. Bieb., a species of juniper with a large spread area in Turkey. Essential oils were extracted by the hydro-distillation method. The components of the hydro distilled oil samples were analyzed by gas chromatography-mass spectrometry (GC-MS). In the study, the essential oil was evaluated for its antibacterial activity against six bacterial strains consisting of two Gram (+) and four Gram (-) by the agar disc diffusion method. Additionally, the antioxidant properties of the oil were determined by TEAC/ABTS + free radical scavenging assay. As a result, there were 27 compounds in the essential oil of J. excelsa. Of the 27 essential oil components identified, α-pinene (40.59%), α-cedrol (18.15%), β-myrcene (4.53%), and limonene (3.84%) were determined as the main components in total 91.54% of the essential oil. As a result, it was observed that the examined juniper essential oil showed a weak but effective antibacterial activity against all bacterial strains compared to the control agents, and also, the examined oil had low but valuable antioxidant activity.
Journal of agricultural …, 2003
The chemical composition of essential oils obtained from Rosmarinus officinalis (family Lamiaceae) and Juniperus Phoenicea (family Cupressaceae) were determined. Their essential oil was determined by hydro-distillation, analysed by GC/MS and GC-FID. The analyses for leaves of Rosmarinus officinalis resulted in the identification of twenty three compounds, representing 63.81% of the total oil and the yields were 0.54%. The major component was α-pinene (18.25%); other predominant components were camphor (6.02%), 1.8-cineole (5.25%), camphene (5.02%), β-pinene (4.58%), bornylacetate (4.35%), limonene (3.56%), borneol (3.10%), α-terpineol (2.89%) and cymene (2.02%). Twenty tree compounds were identified in leaves oil of Juniperus phoenicea representing 81.87% of the total oil composition. The yield of essential oil was 1.62% and the major compound in aerial parts was α-pinene (49.15%) followed by α-phyllandrene (7.39%), mycene (5.24%), β-pinene (3.58%), linalool (2.54%), piperitone (1.56%), γ-terpinene (1.28%), Trans-pinocarveole (1.23%) ρ-cymene (1.10%), α terpineol (1.02%) and γ-cardinene (1.01%).
Antimicrobial activity of juniper berry essential oil (Juniperus communis L., Cupressaceae)
Acta pharmaceutica (Zagreb, Croatia), 2005
Juniper essential oil (Juniperi aetheroleum) was obtained from the juniper berry, and the GC/MS analysis showed that the main compounds in the oil were alpha-pinene (29.17%) and beta-pinene (17.84%), sabinene (13.55%), limonene (5.52%), and mircene (0.33%). Juniper essential oil was evaluated for the antimicrobial activity against sixteen bacterial species, seven yeast-like fungi, three yeast and four dermatophyte strains. Juniper essential oil showed similar bactericidal activities against Gram-positive and Gram-negative bacterial species, with MIC values between 8 and 70% (V/V), as well as a strong fungicidal activity against yeasts, yeast-like fungi and dermatophytes, with MIC values below 10% (V/V). The strongest fungicidal activity was recorded against Candida spp. (MIC from 0.78 to 2%, V/V) and dermatophytes (from 0.39 to 2%, V/V).
2012
The aim of this study was to investigate the chemical composition, antioxidant and antimicrobial activities of the essential oil of Salvia hypargeia from Turkey. According to the results of GC-EIMS analysis, the major compounds of the oil were determined as α-pinene (15.9 %), β-pinene (22.3 %), pulegone (13.5 %) and β-ylangene (17.8 %). Essential oil could not show radical scavenging effect in DPPH system. On the other hand, inhibition capacity of the essential oil against linoleic acid oxidation was measured as 21.0 %. In the case of antimicrobial activity, the most sensitive microorganism was B. subtilis which has the lowest MIC value (35.80 μg/ml). This is followed by S. aureus and C. diphteriae (8.45 and 14.20 μg/ml, respectively).