{"content"=>"Comparative ovicidal activity of leaf extracts on eggs.", "i"=>[{"content"=>"Moringa oleifera"}, {"content"=>"Fasciola gigantica"}]} (original) (raw)
Related papers
Comparative ovicidal activity of Moringa oleifera leaf extracts on Fasciola gigantica eggs
Background: Fasciolosis is an important zoonotic disease affecting the productive performance of farm animals in Egypt. Aim: The aim of the present study was comparing the ovicidal effect of different extracts as an alcoholic (Methanolic and Ethanolic) and aqueous Moringa oleifera leaf extracts on Fasciola gigantica non-embryonated and developed eggs. Materials and Methods: Tested concentrations of extracts ranged from 12.5 to 800 mg/ml. Nitroxynil was used as reference drug with a dose of 100 mg/ml. Results: M. oleifera alcoholic and aqueous extracts showed a concentration-dependent ovicidal effect on F. gigantica non-embryonated and developed eggs. Based on LC50 values, water extract showed the highest ovicidal activity since it registered the lowest values of 2.6 mg/ml on non-embryonated eggs. Non-embryonated eggs were more susceptible to aqueous extract than developed eggs. On the other hand, the developed eggs were more susceptible to ethanolic extract than non-embryonated eggs even the lowest LC50 (12.38 mg/ml). Conclusion: M. oleifera leaf extracts especially aqueous extract could be a promising step in the field of controlling fascioliasis. Further, in vivo studies are needed to enlighten the therapeutic potential of M. oleifera extracts in treating F. gigantica infection. Keywords: Fasciola gigantica, leaf extract, Moringa oleifera, nitroxynil, ovicidal activity.
Journal of Kerbala for Agricultural Sciences
This research aimed to study the impact of spraying different Concentrations of Moringa oleifera leaves Extract on Broiler Breeder Hatching Eggs. The results cleared that, at 18th day the egg weight ranged from 60.50 in the group treated with 5 % M. Water extract to 62.16 gm in control group. While, the egg losses observed in group 4 that, treated with 2 % M. water extract that was 20.56 % at 21st day. The higher hatchability % of total eggs showed in control group that was 69.70 %. While, the hatchability % of fertile eggs showed a higher percentage in group 5 that treated with 5 % M. Water extract that was 99.02 %. The higher percentage of dead embryo observed in control group that was 9.10 % and the lower level observed in the group 2 that treated with 2 % M. Alchole exteract that was 5.46 %. The total aerobic bacteria counts at 18th day of incubation showed a lower level in group 5 that treated with 5 % M. water extract that was 7.88. while, the activity of chicks and in abnorm...
Ciência Rural
ABSTRACT: Fasciolosis has been diagnosed in cattle, goats, sheep and horses in southern and southeastern Brazil. Effective alternative treatments are the targets of study. One promising alternative is the use of plant extracts. The aim of this study was to perform phytochemical analysis of extracts of Eugenia uniflora L., Harpagophytum procumbens, Psidium guajava L. and Stryphnodendron adstringens, and to evaluate the in vitro efficacy of these extracts on ovicidal activity in Fasciola hepatica. Plant extracts were analyzed for phytochemical properties. F. hepatica eggs were collected directly from the gallbladders of animals diagnosed as positive for fasciolosis on post mortem examination. One hundred eggs were incubated with 3 ml of each extract at concentrations of 0.10%, 0.25% and 0.50%, albendazole 0.50% (positive control) or tap water (negative control). To determine anti larval efficacy of each plant extract, hatched eggs were counted and the averages were used. Phytochemical...
Open Life Sciences
Fascioliasis is a disease of livestock which is now recognized as an emerging disease in humans. Cantharellus cibarius and Ganoderma applanatum are known for their medicinal properties. The use of ethanolic extracts of these macrofungi against the eggs and miracidia of Fasciola spp. is a promising method to break the parasite transmission cycle. The aim of the study is to evaluate the inhibitory effects of ethanolic extracts of the mushrooms on eggs and miracidia of Fasciola spp. Concentrated eggs and miracidia of Fasciola spp. were exposed to different concentrations (1-8 mg/ml) of extracts of Ganoderma applanatum (GEE) and Cantharellus cibarius (CEE) at different time intervals. GEE showed superior antiparasitic activities when compared to CEE at all concentrations tested. Significant positive correlations were observed between the concentration of GEE and mortality in miracidia (r=0.980, P <0.05) and CEE and mortality in miracidia (r= 0.968, P <0.05). The study showed that ...
Veterinary Research Communications
Fasciola gigantica is one of the worldwide parasites that cause livestock and human illnesses. Chemotherapy is now the primary therapeutic option for its treatment. Drug abuse has led to the emergence of drug-resistant strains. As a result, there is an urgent need to discover natural and efficient anthelmintics against Fasciola spp. The study aims to evaluate the ovicidal activities of camel milk and its fractions on F. gigantica eggs. In the in vitro assay of F. gigantica eggs were submitted to different concentrations (0.5% and 1%) of camel milk fractions; Camel Milk Whey (CMW), Camel Milk Casein (CMC), and Skimmed Camel Milk (SCM) as well as a positive control (PC) of Nitroxynil (100 mg/ml) and a negative control (NC) with physiological saline. The Egg Hatching Assay (EHA) results showed that camel milk fractions exhibited ovicidal activity, especially CMW, and CMC, which showed 97.58 ± 0.58 and 96.9 ± 1.99 ovicidal activity, respectively, at a concentration of 1% after 15 days o...
American Journal of Plant Sciences, 2014
Haemonchus contortus is a blood-sucking abomasal helminth of small ruminants responsible for major economic losses to small farmers worldwide. Widespread resistance to synthetic anthelminthics has stimulated a need for alternative strategies of parasite control, among which is the use of medicinal plants with natural anthelminthic properties. This study assessed in vitro the efficacy of infused and macerated aqueous extract as well ethanolic extract of Moringa oleifera against fresh eggs, embryonated eggs, L1 and L2 larvae of H. contortus. For this purpose, five different concentrations (0.625, 1.25, 2.5, 3.75 and 5 mg/ml) were prepared from dry extracts via serial dilutions with distilled water. Fresh eggs obtained from artificially infected goat feces were exposed to these different concentrations for 48 hours, while embryonated eggs and larvae were exposed for 6 and 24 hours respectively. Distilled water and 1.5% DMSO were used as negative control. The results were expressed in terms of mean inhibition percentage of egg embryonation, mean inhibition percentage of egg hatch and mean percentage of larval mortality. An overview of results revealed that ethanolic leaf extract of M. oleifera was most efficient on eggs by inhibiting 60.3% ± 8.2% and 92.8% ± 6.2% eggs embryonation at 3.75 and 5 mg/ml respectively with a significant difference (P ˂ 0.05), which contributed to obtaining the lowest LC50 value of 0.985 mg/ml. This extract also inhibited 99% ± 2% egg hatching of H. contortus at 5 mg/ml with an LC50 value of * Corresponding author. G. Mbogning Tayo et al. 1703 against the four developmental stages with LC50 values ranging from 2.08 mg/ml for L2 larvae to 2.92 mg/ml for L1 larvae and 2.37 to 2.52 mg/ml for embryonated and fresh eggs respectively. The current study showed that all three extracts of M. oleifera tested possessed potential ovicidal and larvicidal activities against H. contortus. However, further in vivo studies are necessary to validate the anthelminthic property of this plant.
International Journal of Innovative Science and Research Technology, 2020
One issue affecting the poultry production sectors in developing nations is the rise in feed costs brought on by the high costs of protein and energy sources. Researchers are also looking for organic components that are antibacterial. To cut the expense of the feed, several tropical legumes and plants were added to the diets of chickens as protein sources. Some herbs, spices, and extracts may be coccidiostats, anthelminthic, and antimicrobial, according to recent studies. Most of the tropics are home to the Moringa tree, which has a wide range of applications and is quite significant economically. In order to study its impact on the productive performance of laying hens, nutritionists added it to the diet of poultry. This review's focus is on using Moringa oleifera leaf in diets of laying hens: effect on egg production and quality. Various scientific findings and published research articles were considered about issues such as the study background, objectives, major findings, and review conclusions. M oleifera is also known as a drum stick or miracle tree due to its abundant supply of various nutrients with high biological values. In addition, it has a hypocholesterolemic effect on chickens. It has nutritional as well as therapeutic properties. However, there is still a lot of ambiguity in previously published articles about the major roles of M. oleifera in chicken production performance. Taking this into consideration, the current study provides an outline of the experimental uses of M. oleifera in growth performance, egg production performance, and egg quality, on laying hens, which is supported by previous findings. The gaps in knowledge from previous studies are considered, and the feasibility of M. oleifera in poultry rationing is proposed. The findings have prompted additional research on M. oleifera to determine the most active ingredients and optimal doses in laying hen rations. Finally, the current study shows that M. oleifera supplementation may improve egg quality and production performance.
A STUDY OF EFFECTS OF SEED EXTRACTS OF MORINGA OLEIFERA ON
A STUDY OF EFFECTS OF SEED EXTRACTS OF MORINGA OLEIFERA ON DEVELOPMENT OF AEDES AEGYPTI L, 2019
Aedes aegypti is a vector of dengue, Zika virus and yellow fever, is endemic to Southeast Asia, the Pacific Island, Africa and the Americas. Various synthetic pesticides have been employed against these mosquitoes with an effort to control or eradicate the populations. These pesticides, although highly efficacious against the target species, are facing a threat due to the development of insect’s resistance to these insecticides and degradation effects on the environment (Liu et al., 2006). To prevent proliferation of this mosquito borne diseases and to improve quality of environment and public health, the use of phytochemicals should be prevalent to synthetic insecticides. A study was carried out to determine larval and pupa development of Aedes aegypti in boiled and dissolved seed extracts of Moringa oleifera at varying degree of concentrations. The powdered seeds were measured out in 4, 8 and 12 g and were dissolved in 2 L of distilled cold water for 24, 48 and 72 hours respectively. The same weights of powdered seeds were also put in 2 L of distilled cold water and boiled for 5, 10 and 15 minutes respectively. The result shows that plant seed extracts of M. oleifera, at different concentrations were effective in inhibiting the development of larvae and pupae of A. aegypti.