Moringa oleifera Diets Effect on Haematological Parameters of Rat (Ratus norvegicus) (original) (raw)
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This study was aimed at investigating the effects of Moringa oleifera leaf-meal (MOLM) on some haematological parameters of male albino rats. Sixteen (16) albino rats were randomly selected into four (4) groups; a control group (group A) and three experimental groups (groups B, group C and group D) containing four (4) animals per group. Group A was given normal diet, groups B, C and D were provided with MOLM diets containing 37.5 gm, 56 gm and 75 gm respectively. They were fed with water ad libitum daily for 21 days. At the end of the experimental period, haematological parameters (white blood cell count, packed cell volume, red blood cell count, haemoglobin count and differential white blood cells) were determined. The result showed significant differences (p<0.05) in hemoglobin count, red blood cells and packed cell volume in group B while there was a significant difference (p<0.05) in lymphocyte count of animals in group C and D, although all the groups were within normal range while no significant difference (p >0.05) was shown in the white blood cell count when compared to the control group. In conclusion, this study revealed that MOLM possesses nutritional benefits and this was indicated by its effects on RBC, PCV, haemoglobin and lymphocyte of the animals.
Archives of Applied Science Research, 2013
The experiment was conducted to investigate the effect of Moringa oleifera leaf meal (MOLM) on haematology and serum biochemical parameters of weaner rabbits. Twenty (20) weaned rabbits of mixed breeds, aged between 4- 5weeks with initial average live weight of 627.5g were randomly allocated into four (4) treatment groups with five (5) rabbits per treatment. Each rabbit served as replicate. The rabbits were fed diets containing 0%, 5%, 10%, and 15% Moringa leaf meal in diets designated as T1, T2, T3, and T4 respectively. The experimental diets and clean drinking water were supplied ad-libitum throughout the experimental periods of 8 weeks. The result of the experiment showed no significant (P>0.05) differences among the treatments for packed cell volume (PCV), red blood cell (RBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemolobin concentration (MCHC), white blood cell, lymphocyte, neutrophils, monocytes, basophils and eosinophils. Sig...
Aims: To determine the influence of Moringa oleifera leaf meal (MOLM) on haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. Study Design: Completely randomized design. Place and Duration of Study: At the Rabbit unit of the Teaching and Research farm of Federal College of Agriculture, Ishiagu, Ivo L.G.A., State, Nigeria, between May, 2015 and July 2015. Methodology: 48 growing rabbits were used for this study. Four diets were formulated such that diets T1, T2, T3 and T4 contain MOLM at 0%, 10%, 20% and 30%, respectively. The diets were offered to the growing rabbits, which were randomly divided into 4 groups of 12 rabbits each, with 4 animals constituting a replicate in a completely randomized design pattern. Each animal received the experimental diet for 49 days. Blood samples were drawn from each animal on the last day of Original Research Article Jiwuba et al.; BBJ, 15(2): 1-7, 2016; Article no.BBJ.28095 2 the trial and evaluated for haematological and serum biochemical indices, data obtained were analysed statistically. Results: Results showed that packed cell volume (PCV), haemoglobin Mean cell haemoglobin concentration and white blood cell differed (P<0.05) significantly, while red blood cell, mean cell haemoglobin and mean cell volume were similar (P>0.05) among the groups PCV ranged between 35.01–36.59%. Haemoglobin was improved (p<0.05) by Moringa oleifera leaf meal at 30% inclusion level. White blood cell (WBC) count of weaner rabbits in treatment groups was significantly (p<0.05) higher and better than the control. All the serum biochemical indices were significantly (P<0.05) except creatinine. Cholesterol was lowest at 30% inclusion indicating the anti-diabetic properties of the test ingredient. Conclusions: All the parameters studied fell within the normal range reported for clinically healthy rabbits; an indication that MOLM had a beneficial effect on health status of rabbits. ABBREVIATIONS MOLM = Moringa oleifera leaf meal; PCV = Packed cell volume; MCHC = Mean corpuscular haemoglobin concentration; MCH = Mean corpuscular haemoglobin; MCV = Mean corpuscular volume; RBC = Red blood cell; WBC = White blood cell; ANOVA = Analysis of variance; DM = Dry matter; NFE = Nitrogen free extract; ME = Metabolisable energy; CP = Crude protein; CF= Crude fibre.
Hematological Alterations Occurring in Culinary Exposure of Wistar Rats to Some Indigenous Plants
Journal of Advanced Pharmacy Research, 2024
Background: Traditional medicine has been regarded as a suitable alternative to modern medicine considering the wide acceptance and availability of herbal products across the globe. Many plants have shown hypoglycemic, hypolipidemic, and Insulinogenic properties. However, there is a dearth of information on the hematological alterations following acute culinary exposure of normal rats to these selected medicinal plants. Objectives: This study sought to examine the hematological alterations in Wistar albino rats after culinary exposure to some selected plants with a claim of having medicinal values. Testing of hematological indices can be used to determine the extent of the deleterious effect of foreign compounds including plant extracts on the blood composition of animals. Certain medicinal plants and herbs are believed to enhance health and improve resistance against infection through conditioning the body tissues and re-establishing body equilibrium. Methods: In this study, male albino rats of the Wistar strain were fed diets containing 30% and 70% of weight/weight of plant and animal feeds-Mangifera indica, Peperomia pellucida, Momordica foetida, and Tapinanthus bangwensi-for four days before being killed by cervical dislocation. By puncturing the heart, blood samples were obtained. Using the Auto-Haematology analyzer (URTI) 3300, the significant hematological parameters were examined. Packed Cell Volume (PCV), White Blood Count (WBC), Platelets, and other measurements are among the hematology parameters. Results: The extracts of M. indica, M. foetida, and Viscum album increased red blood cells (RBC), packed cell volume (PCV), and hemoglobin (Hb), among other hematological changes of importance that were seen in the data. Additionally, M. foetida and V. album extracts boosted platelet count. Conclusions: In addition to demonstrating the nontoxic effects of these plants when consumed in moderation, this study provides a key to unlocking novel therapeutic targets for the treatment of anemia and bleeding.
Hematological Indices of Experimental Rats Fed with Rat Chow Fortified with Processed Breadfruits
Asian Journal of Biochemistry, Genetics and Molecular Biology
Background: The abundance of nutrients, vitamins, and fiber in breadfruit has been documented in many literature studies that can hardly be exhausted and are consumed majorly by the Igbos and other southern parts of Nigeria to ameliorate hunger. Aim: This study however was aimed at determining the effect of feeds fortified with grilled, preboiled, and cooked breadfruit on hematological indices of wistar rats. Methods: Hematological parameters were determined using an automated hematology analyzer (Mindray-BC-5300). The parameters analyzed include Hemoglobin (HGB), Packed Cell Volume (PCV), Red Blood Cells (RBC), Platelets (PLT), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), White Blood Cells (WBC), Neutrophils (NEUT), Lymphocytes (LYMPH), Monocytes (MON), Eosinophils (EOS) and Basophils (BAS). Results: The result from the experiment indicated that all fortified groups showed no significant increase or decrease (p&...
Effect of various dietaryprotein levels on haematology and
2016
Protein is very crucial for growth of birds.Blood parameters are used as indicator for diagnosis of diseases. Haematological values also reflect the levels of stresses due to nutritional and environmental factors. The present study was conducted to evaluate the effect of various dietary protein levels on haematological and blood biochemistry values in different close bred flocks of Japanese quail. One thousand and eighty, day-old chicks from four different close bred flocks comprising 270 chicks having 135 of each separate sex (♂ and ♀) were divided into 3 subgroups assigning 3 different protein levels (21, 23, 25%) having 3 replicates comprising 15 birds each. From 4th week onward 72 birds (randomly picking one bird from each replicate) were slaughtered at the end of each week. The blood samples were collected in the tubes from the jugler vein by slaughtering the birds. The blood was analysed following standard procedure to study the haematological and blood biochemistry. The results showed that strains of Japanese quail did not differ significantly in haematological parameters andblood cholesterol level from 4 th to 6 th week in both sexes. However, strains differ in total protein, albumin and globulin content in blood. Dietary protein level significantly affected most of haematological and biochemical parameters of blood. Highest protein level (25%) showed best performance in all the parameters except hemoglobin and cholesterol level. It is concluded that 25% protein level in feed is best for rearing of Japanese quail for meat purpose in tropics.
The use of medicinal plants as food supplements and in the treatment of specific diseases dates back to antiquity. Age old anecdotal reports from many cultures strongly suggests a role for diet of plant origin in preventive and therapeutic medicine. Anaemia remains a major public-health problem especially in developing countries. This study aimed at investigating the antianaemic potentials of combined diet of garlic (Allium sativum), garden egg (Solanum melongena) and groundnut (Arachis hypogaea).Twenty five wistar rats of both sexes weighing 160-200g were divided equally into five groups. Group 1 served as the non-anaemic control and fed with normal rat chow. Groups 2, 3, 4 and 5 were induced with anaemia by oral administration of phenylhydrazine (PHZ) given at 50mg/kgBW. Group 2 served as anaemic control, fed with normal rat chow; Group 3 were fed with 20g (75%ww) of garlic, garden egg and groundnut in the ratio of 1:1:1 with normal rat chow; Group 4 fed with 10g of garden egg (50%ww) in the ratio 1:1 with normal rat chow and Group 5 fed with 10g of ground, nut (50%ww) in the ratio 1:1 with normal rat chow. Animals were sacrificed at the end of fourteen days of dietary feeding using chloroform vapour with blood collected at the left ventricle to test for haematological parameters such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin concentration (Hb) and packed cell volume (PCV). Results showed that RBC, WBC, Hb and PCV values increased in all the experimental as compared to anaemic control with a significant (P<0.05) in WBC values of groups 3 and 4 as compared to the anaemic control. This suggests a consumption of garlic, garden egg and groundnut will moderately improve anaemic condition.
Effect of Moringa oleifera aqueous leaf extract on some haematological indices in Wistar rats
The use of Moringa oleifera leaf extract in the treatment of virtually all ailments calls for further research to support the claim for its ability to boost blood. Thirty six albino rats of the Wistar strain weighing 200-230g were sorted into groups according to their weights and sex. The animals were divided into two groups, male and female. Three (3) test groups (1%, 5%, and 10% w/w) for the males containing five (5) animals each and a control group, for the females, three (3) test groups (1%, 5%, and 10% w/w) and control group with four (4) animals each. The result of haematological indices in female Wistar rats administered aqueous extract of M. oleifera shows that Hb count generally increased significantly in all the groups compared with their control group. Similarly, PCV and WBC generally increased in all the groups, this increase was significant for WBC in all the groups. RBC values showed a non-significant (p>0.05) value at 1% treated group but a significant increases (p...
Effects of commonly used food additives on haematological parameters of Wistar rats
Heliyon, 2020
This study was done to investigate the effects of common food additives such as sodium benzoate (SB) and ascorbic acid (AA) on haematological parameters of male Wistar rats. Forty-eight (48) male albino rats with an average weight of 105 g were grouped into twelve (n = 4) of Basal Control and other 11 groups orally administered 1 mg of SB, 10 mg of SB, 10 mg of AA, 0.2 mg of AA + 0.5 mg of SB, 0.2 mg of AA + 1 mg of SB, 0.2 mg of AA + 10 mg of SB, 0.2 mg of SB + 0.1 mg of AA, 0.2 mg of SB + 0.5 mg of AA, carbonated soft drinks (CSD)+ 0.1 mg of AA, CSD + 1 mg of AA and CSD + 10 mg of AA, respectively for 21 non-consecutive days. At the end of the experiment, blood samples were collected in EDTA anticoagulant tubes, haematological parameters were evaluated, and data were analyzed. There was a dose-dependent increase (p < 0.05) in White Blood Cell counts of SB treated rats compared with the control group. The lymphocyte exhibited significant reduction (p < 0.05) in the groups tre...
NUTMEG ON HAEMATOLOGICAL PARAMETER
In spite of wide spread biological uses of Myristica fragrans (nutmeg), there is a dearth of information on its effects on haematological indices. This work was therefore conducted to evaluate the effects of ethanolic extract of M. fragrans on some haematological indices using albino rat as a model. Twenty four (24) Wistar strain albino rats weighing 140 to 160 g were randomly distributed into four (4) groups of six (6) animals per group. Group I consists of rats which received 10 ml/kg normal saline (orally) and served as the control while those in Groups II, III and IV received 50% ethanolic seed extract of M. fragrans (orally) at doses of 100, 250 and 500 mg/kg, respectively. In all groups, the blood samples were obtained by cardiac puncture for analysis of haematological indices after feeding regimens lasted for 14 days. The results showed significant decreases (p< 0.05) in red blood cell (RBC) count, packed cell volume (PCV), haemoglobin concentration (HbC) and platelet count especially at high doses. There was significant increase (p< 0.05) in total white blood cell (WBC) count. This study therefore seems to confirm the anti-inflammatory properties of seed of M. fragrans and also suggests that it may have deleterious effects on haemopoiesis at high doses.