Structure Dynamics Guided Enzyme Improvement of ENDO-BETA-1, 4-XYLANASE I (original) (raw)

Enzyme structure dynamics has recently been revealed to be essential for structure-function relationship. Among various structure dynamics analysis platforms, hydrogen deuterium exchange mass spectrometry stands as an efficient and highthroughput way to analyze protein dynamics upon ligand binding, protein folding, and enzyme catalysis. HDX-MS can be used to study the regional dynamics of proteins based on the m/z value or percentage of deuterium incorporation for the digested peptides in the HDX experiments. Various software packages have been developed to analyze HDX-MS data. However, for the accurate, enhanced, and explicit statistical analysis of HDX-MS data statistical analysis of software was developed as HDXanalyzer. v ACKNOWLEDGEMENTS I would like to express my deep gratitude to my advisor Dr. Joshua S. Yuan, for his invaluable guidance and suggestions, his constant encouragement and financial support, and his understanding, patience, and friendship in my pursuit of a PhD degree. I