A Review on Mass Spectrometry: Its tools and data analysis for proteomics (original) (raw)

A Review on Mass Spectrometry: Technique and Tools

Protein structure prediction has gain important in area of life sciences, because of its complex structure. The protein-protein interaction is necessary to study the behavior of protein in a specific environment, and study molecular relationship in living systems. Therefore, large scale proteomics technologies are required to measure physical connection of proteins in living organisms. Mass Spectrometry uses the technique to measure mass-tocharge ratio of ion. It's an evolving technique for characterization of proteins. A Mass Spectrometer can be more sensitive and specific, also complement with other LC detectors. Liquid Chromatography, unlike gas chromatography is a separation technique which helps to separate wide range of organic compounds from small molecular metabolites to peptides and proteins. This paper addresses the study of data analysis using mass Spectrometry. It also includes the study of various methods of Mass Spectrometry data analysis, the tools and various applications of Mass Spectrometry.This review briefs on Mass Spectrometry technique, its application, usage, and tools used by Mass Spectrometry.

Analyzing peptides and proteins by mass spectrometry: principles and applications in proteomics

2012

The study of proteins has been a key element in biomedicine and biotechnology because of their important role in cell functions or enzymatic activity. Cells are the basic unit of living organisms, which are governed by a vast range of chemical reactions. These chemical reactions must be highly regulated in order to achieve homeostasis. Proteins are polymeric molecules that have taken on the evolutionary process the role, along with other factors, of control these chemical reactions. Learning how proteins interact and control their up and down regulations can teach us how living cells regulate their functions, as well as the cause of certain anomalies that occur in different diseases where proteins are involved. Mass spectrometry (MS) is an analytical widely used technique to study the protein content inside the cells as a biomarker point, which describes dysfunctions in diseases and increases knowledge of how proteins are working. All the methodologies involved in these descriptions are integrated in the field called Proteomics.

Peptide and protein analysis with mass spectrometry

Spectroscopy, 2002

Mass spectrometry (MS) is rapidly becoming a fundamental tool for biologists and biochemists in their efforts to characterize cellular function. Recent advancements in MS technology and front-end methodologies, along with the completion of the human genome have greatly popularized its use by researchers for protein identification and characterization. This paper is a general overview of how mass spectrometry is being used for the analysis of peptides and proteins, focusing on its application to molecular weight determination. Sample preparatory and cleanup techniques used in our laboratory for protein and peptide analysis are provided, along with a discussion of data interpretation. The utility of mass spectrometry for protein and peptide analyses lies in its ability to provide highly accurate molecular weight information on intact molecules. The ability to generate such accurate information can be extremely useful for protein identification and characterization. For example, a prot...

Mass Spectrometry: An Essential Tool for Genome and Proteome Analysis

2003

Mass spectrometry (MS), in its various forms, has become an essential tool for genome and proteome analysis. It involves gaseous ionization of the analyte to be examined, followed by separation of ions according to mass-to-charge (mlz) ratio and determination of molecular masses of iOIlSfrom mass spectra obtained after mass spectrometry of analyte. Several methods for ionization, mainly including MALDI and ES, each coupled with a specific mass spectral analysis system (e.g. TOF-MS and quadrupole MS) are available. MS/MS is devised particularly for the determination of amino acid sequences of small peptide. The advantage of MS over other techniques is its speed, since gel electrophoresis and labeling of the analyte, needed in other techniques used for genome/proteome analysis, can be dispensed with. Applications of mass spectrometry for genome analysis include DNA sequencing and SNP detection, the latter involving PinPoint assay (minisequencing), PNA hybridization, invader cleavage, ...

Mass Spectrometery as an Analytical Method for Determining Protein Structures

Journal of Bioengineering & Biomedical Science, 2015

Mass spectrometry (MS) has become a powerful technology in the discovery and development of protein therapeutics in the biopharmaceutical industry. This review article describes the two main types of mass spectrometry methods for determining the protein structure. Further the role of tandem mass spectrometry in diagnostics has also been highlighted. Mass spectrometry based proteomics is a central life science technology that has realized great progress towards identification, quantification and characterization of the proteins that constitute a proteome.