A Review on Mass Spectrometry: Its tools and data analysis for proteomics (original) (raw)

With advent of „The Human Genome Project‟ large-scale proteomics has rapidly come to dominate the post genomic age. The Protein Prediction is hard because of its complex structure. Data Analysis is the main issue while predicting the protein. The main problem in proteomics is to estimate the several proteins available in cell structure or tissue sample. Therefore, large scale proteomics technologies are required to measure physical connection of proteins in living organisms. Mass Spectrometry uses the technique to measure mass-to-charge ratio of ion. It‟s an evolving technique for characterization of proteins. A Mass Spectrometer can be more sensitive and specific, also complement with other LC detectors. Liquid Chromatography, unlike gas chromatography is a separation technique which helps to separate wide range of organic compounds from small molecular metabolites to peptides and proteins. This paper addresses the study of data analysis using mass Spectrometry. It also includes th...