The role of E receptors in the attachment of thymocytes and T lymphocytes to human target cells (original) (raw)
1983, Clinical immunology and …
Human thymocytes, activated T lymphocytes, and neuraminidase-treated T cells possess the distinct capacity of forming conjugates with various human cell lines. The present study investigated whether E receptors, which endow human T cells with their capacity to bind sheep red blood cells (SRBC), are involved in this phenomenon. Monoclonal antibodies to human T cells and various simple sugars were studied for their effect on the attachment of human T cells to target cells. A-22, a monoclonal antibody to the E receptor, inhibited the formation of E rosettes by T cells and SRBC, and reacted in immunofluorescent-staining assays with the majority of human thymocytes and peripheral T cells, and with T-cell lines capable of forming E rosettes. When human thymus cells were treated with A-22 antibody they showed a reduction of up to 70% in their capacity to attach to the GM-4762 lymphoblast cell line and the K-562 myeloid line. Antibody treatment of the target cells, rather than of the thymus cells, had no effect on the formation of conjugates between thymus cells and target cells. Treatment of thymus cells with various monoclonai antibodies to T cells which do not react with the E receptor had no inhibitory effect. The exposure of human thymus cells to various simple sugars (D-fWinnOSe, n-fucose, galactose, and lactose) markedly reduced their capacity of forming conjugates with target cells. Exposure of neuraminidase-treated peripheral blood lymphocytes and of activated T cells to A-22 antibody inhibited their attachment to human target cells.
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Immunoregulation of the proliferative response of lymphocytes by soluble E receptors
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The role played by soluble human T-lymphocyte receptors for sheep erythrocytes (E) upon the proliferative response of lymphocytes was investigated. Preparations containing soluble E receptors, e.g., normal human serum dialysate (NHSD) and leukocyte dialysate containing transfer factor (TF) were added to cultures performed in the presence of normal human serum. In most of the cultures stimulated by allogeneic cells or by phytohemagglutinin (PHA), the addition of these preparations induced inhibition of the [3H]thymidine uptake. Previous absorption with E abolished or diminished significantly this inhibitory effect. Addition of NHSD absorbed with E restored the proliferative response in mixed leukocyte cultures performed in medium containing dialyzed normal human serum instead of NHS. The immunoregulation exerted by soluble E receptors upon the proliferative response of human lymphocytes seems to be more clearly expressed in the response to allogeneic cells than in the response to PHA.
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Cell to Cell Interaction in the Immune Response: V. Target Cells for Tolerance Induction
Journal of Experimental Medicine, 1970
Collaboration between thymus-derived lymphocytes, and nonthymus-derived antibody-forming cell precursors occurs during the immune response of mice to sheep erythrocytes (SRBC). The aim of the experiments reported here was to attempt to induce tolerance in each of the two cell populations to determine which cell type dictates the specificity of the response. Adult mice were rendered specifically tolerant to SRBC by treatment with one large dose of SRBC followed by cyclophosphamide. Attempts to restore to normal their anti-SRBC response by injecting lymphoid cells from various sources were unsuccessful. A slight increase in the response was, however, obtained in recipients of thymus or thoracic duct lymphocytes and a more substantial increase in recipients of spleen cells or of a mixture of thymus or thoracic duct cells and normal marrow or spleen cells from thymectomized donors. Thymus cells from tolerant mice were as effective as thymus cells from normal or cyclophosphamide-treated ...
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