The Chemical Structure of the Lipopolysaccharide of a Rc-Type Mutant of Proteus mirabilis Lacking 4-Amino-4-Deoxy-L Arabinose and Its Susceptibility towards Polymyxin B (original) (raw)

Springer eBooks, 1990

Abstract

The mutant Proteus mirabilis R4 (R4/028) was obtained from the wild-type strain P. mirabilis 028 (F87) by ultraviolet irradiation. Isolation of R4/028 lipopolysaccharide (LPS) and the partial elucidation of the glucose-heptose region as a trisaccharide: β-glucosyl-(1→ 3/4)-L-glycero-α -D-manno-heptosyl-(1 → 4/3)-L-glycero-α-D-manno-heptosyl-7-phosphate has already been described (11). The linkage region between d0clA and heptose, the terminal and side chain-linked d0clA, the substituents of the phosphate groups and the lipid A structure were the aim of this study. In addition, we examined the effect of polymyxin B on P. mirabilis R4/028 mutant, after finding that its LPS is lacking 4-amino-4-deoxy-L-arabinose (Ara4N). The presence of that unusual aminopentose has been suggested by Vaara et al., (15, 16) to be the reason for the resistance of P. mirabilis strains towards the action of polymyxin.

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