Preparation, Release Pattern and Antibacterial Activities of Chitosan-Silver Nanocomposite Films (original) (raw)
Related papers
2018
A simple and eco-friendly approach has been successfully developed for the preparation of chitosan nanocomposites films loaded with silver nanoparticles using kumquat extract as a biological reducing agent. The chitosan/silver nanocomposites (CTS/Ag NCPs) films were prepared from chitosan/silver nanocomposites solution and dried for 14 h at 70C in a vacuum oven with the pressure of 0.03 Mpa. The morphology and characterization of CTS/Ag NCPs films have been also determined by FTIR, XRD, and SEM. The UV-vis spectroscopy and TEM image indicated that synthesized chitosan/silver nanocomposites have spherical shape with their uniform dispersion and their average particle size of about 20-30 nm. The prepared CTS/Ag NCPs films showed their great antibacterial activity on Staphylococcus aureus (S. Aureus) and Escherichia coli (E. coli). Therefore, this eco-friendly method that would be used for the preparation of chitosan/Ag nanocomposites films could be competitive and alternative to the e...
Colloids and Surfaces B-biointerfaces, 2010
The present investigation involves the synthesis of porous chitosan-silver nanocomposite films in view of their increasing areas of application in wound dressing, antibacterial application, and water purification. The entire process consists of three-steps including silver ion-poly(ethylene glycol) matrix preparation, addition of chitosan matrix, and removal of poly(ethylene glycol) from the film matrix. Uniform porous and brown colour chitosan films impregnated with silver nanoparticles (AgNPs) were successfully fabricated by this facile approach. Both, poly(ethylene glycol) (PEG) and chitosan (CS) played vital roles in the reduction of metal ions into nanoparticles (NPs) as well as provided good stability to the formed nanoparticles. The developed porous chitosan-silver nanocomposite (PCSSNC) films were characterized by UV-vis and FTIR spectroscopy, and thermogravimetric analysis for the confirmation of nanoparticles formation. The morphology of silver nanoparticles in nanocomposite films was tested by optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The embedded AgNPs were clearly observed throughout the film in SEM and the extracted AgNPs from the porous chitosan-silver nanocomposite showed ∼12 nm in TEM. Improved mechanical properties were observed for porous chitosan-silver nanocomposite than for chitosan blend (CSB) and chitosan-silver nanocomposite (CSSNC) films. Further, the examined antibacterial activity results of these films revealed that porous chitosan-silver nanocomposite films exhibited superior inhibition.
Journal of Agricultural and Food Chemistry, 2013
This study aims to develop antimicrobial films consisting of chitosan and silver nanoparticles that are homogeneously distributed throughout the polymer matrix. Nanoparticles were generated in situ during the neutralization of the chitosan acetate film with sodium hydroxide. The temperature of neutralization and the concentration of silver in the film were crucial determinants of the shape and size of the nanoparticles. Neutralized films exhibited antimicrobial activity against Escherichia coli and Staphylococcus aureus in liquid growth media. However, the effectiveness of the films was considerably greater in diluted growth media. Furthermore, no significant differences were found either in the antimicrobial capacities of films incorporating different amounts of silver or in the amount of silver that migrated into the liquid media after 18 h of immersion of the film. Neutralized films maintained their activity after 1 month of immersion in deionized water, which can be attributed to the slow sustained release of silver ions and thus efficacy over time. KEYWORDS: synthesis, silver-based nanoparticles, chitosan, antimicrobial activity ■ MATERIALS AND METHODS Synthesis of Chitosan/Silver-Based Nanoparticle Films. Low molecular weight chitosan (MW 50−190 kDa, 75−85% deacetylated) from shrimp shells, sodium hydroxide (ACS reagent, ≥97.0%, pellets), and silver nitrate (ACS reagent, ≥99.0%) were obtained from Aldrich Chemical Co., Inc., Milwaukee, WI, USA. A 1.5% (w/w) chitosan solution was prepared in 0.5% (w/w) acetic acid and stirred at 40°C for 1 h. After the solution had cooled to room temperature, silver nitrate, previously diluted with a small amount of distilled water, was added to the solution and left shaking, protected from light, until complete dissolution. Several chitosan solutions with different silver
Synthesis of Chitosan-silver nanocomposites and their antibacterial activity
International Journal of Scientific and Engineering Research
The present study explores the in situ fabrication of chitosan-silver nanocomposites in view of their increasing applications as antimicrobial packaging, wound dressing and antibacterial materials. Chitosan/Silver nanocomposites were prepared by embedding of silver nanoparticles in chitosan polymer. Synthesis of nanocomposites was confirmed by Fourier Transform Infrared (FTIR) spectroscopy, X-Ray Diffraction (XRD) analysis and Differential Scanning Calorimetry (DSC) etc. In addition, the formed nanocomposites have an average particle size of ~10-15 nm as observed by Transmission Electron Microscopy (TEM). Their antibacterial activity was assessed by zone of inhibition method against Staphylococcus aureus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella entrica MTCC 1253 in vitro.
Chitosan–silver oxide nanocomposite film: Preparation and antimicrobial activity
Bulletin of Materials Science, 2011
The chitosan-silver oxide encapsulated nanocomposite film was prepared by solution casting method. The prepared film was characterized by FTIR, scanning electron microscopy (SEM), thermal studies, and UV-Vis spectroscopy. The elemental composition of the film was studied by energy dispersive X-ray analysis (EDAX). The antibacterial activity of the composite film against pathogenic bacteria viz. Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa was measured by agar diffusion method. Our observations suggest that chitosan as biomaterial based nanocomposite film containing silver oxide has an excellent antibacterial ability for food packaging applications.
Journal of Biomaterials Science-polymer Edition, 2009
In this work chitosan/silver nanoparticle films were synthesized by a simple photochemical method of reduction of silver ions in an acidic solution of AgNO 3 and chitosan. This is a novel, cheap, easy, quick and in situ approach to prepare chitosan films loaded with silver nanoparticles (AgNPs). Chitosan used here is a natural polymer and acts as a very good chelating and stabilizing agent; thus, this approach of formation of chitosan/silver nanoparticle films is proved to be an excellent 'green approach' for the synthesis of metal nanoparticle composites. The presence of silver nanoparticles was confirmed from the transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA) of the film. The surface plasmon resonance (SPR) obtained at 400 nm also confirmed the presence of nanosilver in the chitosan film. The developed chitosan-nanosilver films demonstrated excellent antibacterial action against model bacteria, Escherichia coli and Bacillus. This approach can be easily used in the large-scale production of such silver-nanoparticles-loaded chitosan films. These films can be used as antimicrobial packaging materials, as wound dressings and can also be grafted onto various implants.
International Journal of Biological Macromolecules, 2011
The present work describes the preparation of new chitosan complex with 4-(ethoxycarbonyl) phenyl-1-amino-oxobutanoic acid (ETHA), as a matrix for silver nanoparticles to obtain a nanocomposite film by solution casing method. The characterization of the prepared nanocomposite film was made by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetry (TG) and scanning electron microscopy (SEM). The optical property of nanocomposite film was analyzed by UV–Visible and photo-luminescence (PL) spectroscopy. The nanocomposite film was screen for antibacterial activity with Staphylococcus aureus (gram positive), Pseudomonas aurigionasa (gram negative) and Escherichia coli (gram negative) bacteria by adopting the disk diffusion method. The result of antibacterial study revealed that the prepared nanocomposite film may be a promising candidate for wide range of bio-medical applications.► The successful preparation of 4-(ethoxycarbonyl) phenyl-1-amino-oxobutanoic acid–chitosan complex as a matrix for silver nanocomposite film is done. ► The nano size silver particles has been easily been dispersed in the chitosan complex. ► The result of antibacterial study revealed that the prepared nanocomposite film is more effective in comparison to all of its individual components. ► The prepared nanocomposite film may be useful for biomedical applications.
Factors Affecting the Antibacterial Activity of Chitosan-Silver Nanocomposite
IET Nanobiotechnology, 2017
This study provides the optimum preparation parameters of chitosan-silver nanoparticles composite (CSNC) with promising antibacterial activity against the most common bacterial infections found on burn wounds. CSNC was synthesised by simple green chemical reduction method with different preparation factors. Chitosan was used to reduce silver nitrate and stabilise silver nanoparticles in the medium. For this reason, spectroscopic and microscopic techniques as, ultraviolet-visible Fourier transform infrared spectroscopy and transmission electron microscopy were used in the study of the molecular and morphological properties of the resultant composites. Furthermore, the composite was assessed in terms of Ag-ions release by AAS and its efficacy as antibacterial material. As a result, CSNC showed stronger antibacterial effect than its individual components (chitosan and silver nitrate solutions) towards Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacteria. CSNC prepared in this study showed highest inhibition percentage of bacterial growth up to 96% at concentration of 220 μg/ml.
Synergistic antibacterial activity of chitosan–silver nanocomposites on Staphylococcus aureus
Nanotechnology, 2011
The approach of combining different mechanisms of antibacterial action by designing hybrid nanomaterials provides a new paradigm in the fight against resistant bacteria. Here, we present a new method for the synthesis of silver nanoparticles enveloped in the biopolymer chitosan. The method aims at the production of bionanocomposites with enhanced antibacterial properties. We find that chitosan and silver nanoparticles act synergistically against two strains of Gram-positive Staphylococcus aureus (S. aureus). As a result the bionanocomposites exhibit higher antibacterial activity than any component acting alone. The minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of the chitosan-silver nanoparticles synthesized at 0 • C were found to be lower than those reported for other types of silver nanoparticles. Atomic force microscopy (AFM) revealed dramatic changes in morphology of S. aureus cells due to disruption of bacterial cell wall integrity after incubation with chitosan-silver nanoparticles. Finally, we demonstrate that silver nanoparticles can be used not only as antibacterial agents but also as excellent plasmonic substrates to identify bacteria and monitor the induced biochemical changes in the bacterial cell wall via surface enhanced Raman scattering (SERS) spectroscopy.
European Polymer Journal
n the present work chitosan-silver (CS/Ag) nanocomposites, either in the form of nanoparticles (AgNP) or as ionic dendritic structures (Ag+), are synthesized by a simple and environmentally friendly in situ chemical reduction process. The antibacterial activity of the resulting nanocomposites in the form of films is studied against two bacteria, Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The relationship between electrical, structural and antibacterial properties of CS/AgNP and CS/Ag+ nanocomposites are studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction, and UV-Vis, impedance, infrared and X-ray photoelectron spectroscopies. The results demonstrate that in contrast to CS/Ag+ ion films, the CS/AgNP composites films (average particle size less than 10 nm) showed a significantly higher antibacterial potency. The collective action of AgNP and Ag+ ions facilitate the enhancement and synergetic antibacteria...