immunoautora (original) (raw)

2016

Abstract

We have initiated immunological and drug- binding studies on the tubulins from different higher plant species. Antibodies were raised against electrophoretically sep- arated rose (Rosa sp.) tubulin a- and 13-subunits and charac- terized by immunoblot autoradiographic assays. Each IgG preparation bound to its antigen and cross-reacted differen- tially with the respective tubulin subunits from an alga, sea urchin, rabbit, and cow. Antigenic determinants were shared more among the ,f-subunits than among the a-subunits from these organisms. Tubulins were isolated from cultured cells of carrot (Daucus carota) and hibiscus (Hibiscus rosa-senensis). Immunoautoradiography and quantitation of cross-reactivity on blots showed nonidentity among homologous subunits from rose, carrot, hibiscus, and alga tubulins, with more antigenic differences among a-subunits than among P-subunits. Com- parative colchicine-binding assays showed that rose and hibis- cus tubulins bound 33% and 65%, respectively,...

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