Fungal Strain as Biological Tool to Remove Genotoxicity Effect of Phenolic Compounds from Olive Mill Wastewater (original) (raw)
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Effect of Adding Microorganisms on the Degradation of Phenolic Compounds in Olive Mill Wastewater
Iraqi Journal of Science
Bio-treatment is considered as one of ecologically most efficient methods of wastewater treatment. This study was done in order to reduce the negative effects phenolic compounds included in the olive mill wastewater added to the cultivated soil and to study the individual and collective ability of fungal and bacterial isolates to dismantle them. The experiment, conducted in 2020 with randomization in experimental design, consisted of six treatments and three replications. First four treatments had olive mill wastewater treated with fungal isolates Penicillium sp, Aspergillus flavos and bacterial isolates Pseudomonas sp,Bacillus sp individually. The fifth treatment included collective use of both isolates, in addition to the control which was without any treatment. The results of the statistical analysis showed that the phenolic compounds amount remained in the treated water which indicated the superiority of Penicillium sp over all treatments, where the apparent superiority of ...
DESALINATION AND WATER TREATMENT, 2019
The present work aims to evaluate the capacity of a yeast strain to degrade the olive mill wastewater (OMWW) and to obtain a stabilized product that can be disposed without any environmental impact on the water-soil-plant system. The pollutant load, expressed by the chemical oxygen demand (COD) and the phenolic compounds (PC), are 187.63 and 4.30 g/L, respectively. The evaluation of the yeast strain capacity to develop on liquid and solid culture media based on the OMWW at different dilutions of 25%, 50%, 75%, raw matter, allowed us to optimize the abatement of the pollutant load, particularly, PC. The results show a significant reduction of 98% for the COD and 97% for the PC, after 1 month of treatment at a dilution of 25%. The PC extracts were characterized by high-performance liquid chromatography, the main identified PC are hydroxytyrosol, tyrosol, caffeic acid, p-coumaric acid and oleuropein. Most of which had disappeared after the treatment. The selected yeast strain was identified by 26S rDNA sequencing and shows that belongs to the species of Rhodotorula mucilaginosa.
Problems of identifying phenolic compounds during the microbial degradation of olive mill wastewater
International Biodeterioration & Biodegradation, 1996
The main objectives of the presented Spanish-German collaboration are the purification of alpechin by biodegrading phenolic compounds and the investigation of metabolites during fermentation prior to its safe disposal. In addition to 12 well-known compounds, 3,4-dihydroxyphenylglycol was also identified in untreated Spanish and Italian alpechin samples using a GC/MS method. The qualitative composition of the Italian and Spanish samples differ. First results of degradation tests of reference substances are reported: Arthrobacter is capable of completely transforming added tyrosol to the corresponding 4-hydroxyphenylacetic acid and after 139h of fermentation no traces of tyrosol can be identified. In contrast, only traces of phenylacetic acid are produced by Bacillus pumilus after 139h of fermentation of tyrosol.
Biodegradation of Toxic Compounds in Olive Mill
2013
Thirty-two strains isolated from olive oil production plants were screened for their tolerance to the polyphenols and their ability to grow in olive oil mill wastewaters (OMW). An Ascomycete isolate that grew best on OMW was selected for further study to evaluate its effect on removal of organic pollutants, phenolic compounds and OMW toxicity. This strain was identified by DSMZ as Aspergillus niger van Tieghem. The growth of Aspergillus niger van Tieghem on stored OMW decreased pH and led to the reduction of phenolic compounds with a resultant discoloration of OMW and significant reduction of chemical oxygen demand (COD), in proportion to the dilution of OMW. The reduction of the COD, phenolics and color exceeded 50%. Tannase, the enzyme detected in the growth medium of Aspergillus niger van Tieghem was produced during primary metabolic growth. The maximal enzyme activity attaining 0.68 EU/ml was achieved in 3rd days of cultivation. The phytotoxicity of the stored OMW was reduced to about 40% after 4 days' incubation with Aspergillus niger van Tieghem.
Process Biochemistry, 2000
The manufacturing process of olive oil yields a liquid waste called 'olive mill wastewater' (OMW). This waste has a high polluting power as well as a high antibacterial activity exerted, among others, by various phenolic compounds. Among the methods for the purification of OMW, biological systems show some advantages that make these particularly suitable. However, the presence of phenolic inhibitors requires the use of microorganisms able to utilise aromatic compounds. The capability and kinetic behaviour of several fungi to deal with such a waste reducing the phenol content of OMW was studied. The removal of total phenols relative to the total organic load consumed, which might indicate a measure of the selectivity with which the microorganisms remove phenols among other organic compounds present, indicates the sequence: Phanerochaete chrysosporium \ Aspergillus niger \Aspergillus terreus.
Journal of Chemical Technology & Biotechnology, 2004
This study deals with the degradation of phenolic compounds in olive oil mill waste; a highly polluting material in olive oil-producing countries because of its abundance and the toxicity of its phenolic compound content. This investigation confirms the ability of an isolated yeast, identified as Trichosporon cutaneum, to degrade phenolic compounds extracted from olive mill waste water (OMW). The yeast was adapted to the OMW by an enrichment culture. The results of this biotransformation were a decrease in the phenolic content and hence a reduction in the phytotoxic effects of the effluent after the yeast treatment. The kinetic growth of the isolated yeast on phenol over a range of concentrations (0.3-3.0 g dm −3) was studied. The ability of the strain to assimilate simple monomeric phenols and alkyl phenols, at a concentration of 1 g dm −3 , in a synthetic liquid medium used as the sole carbon source was investigated in a batch culture. The aromatic ring cleavage pathway occurred in the yeast through catechol oxidation. Using various concentrations of ethyl acetate extract from OMW as the sole carbon source, the yeast exhibited growth on the substrate up to 7 g dm −3 equivalent of phenols. A significant reduction of COD after the treatment of the OMW extract by the yeast isolate was noticed. The removal of phenol and COD exceeded 80% of the original loading after 8 days of treatment, for extracts containing initial COD in the range 19 to 72 g dm −3 .
Screening and selection of fungi for bioremediation of olive mill wastewater
World Journal of Microbiology and Biotechnology, 2010
Olive mill wastewater (OMWW) is a significant pollutant and its phytotoxicity is attributed mostly to the phenols present. 220 fungi were screened for their ability to produce detoxifying enzymes and/or grow in OMWW. Four isolates, species of Cerrena, Byssochlamys, Lasiodiplodia and Bionectria were selected and compared against Phanerochaete chrysosporium for their ability to bioremediate OMWW in the presence of a competing indigenous microflora. For the first time we report that a Cerrena sp. achieved a 75% reduction of phenolics in OMWW and that, unusually, the reduction occurred within 2 h of the addition to the OMWW.
Microbial Application to Improve Olive Mill Wastewater Phenolic Extracts
Molecules, 2021
Olive mill wastewater (OMW) contains valuable and interesting bioactive compounds, among which is hydroxytyrosol, which is characterized by a remarkable antioxidant activity. Due to the health claims related to olive polyphenols, the aim of this study was to obtain an extract from OMW with an increased level of hydroxytyrosol by means of microbial enzymatic activity. For this purpose, four commercial adsorbent resins were selected and tested. The beta-glucosidase and esterase activity of strains of Wickerhamomyces anomalus, Lactiplantibacillus plantarum, and Saccharomyces cerevisiae were also investigated and compared to those of a commercial enzyme and an Aspergillus niger strain. The W. anomalus strain showed the best enzymatic performances. The SP207 resin showed the best efficiency in selective recovery of hydroxytyrosol, tyrosol, oleuropein, and total phenols. The bioconversion test of the OMW extract was assessed by using both culture broths and pellets of the tested strains. ...
Suitability of yeasts for the treatment of olive mill wastewater
2010
Olive mill wastewater (OMW) is strongly hindered by high concentration of phenolics, which is considered the main cause of its toxicity. Despite this high level of phenolics, yeasts are the dominant microorganisms with levels as high as 10 colony forming units/ml. The identification of yeast isolates from freshly produced OMW from olive oil plants, stored OMW in ponds and larvae of the olive fly Bactrocera oleae revealed that many species are common in these environments along with olives. Some strains of Candida holstii, C. diddensiae, C. boidinii, Pichia guillermondii and Geotrichum candidum were able to grow and reduce phenolics concentration in the OMW. However, phenolics reduction was lower than 50% raising the question if yeasts degrade or simply adsorb the phenolics to their cell walls. A review of the literature reveals that a few species of Trichosposron cutaneum and more recently Candida tropicalis, C. matosa, and C. aquatextoris can grow solely on phenol and other aromati...