Effect of linseed diet and hazelnut skin supplementation on oxidative status and blood serum metabolites in rabbits (original) (raw)
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Egyptian Journal of Nutrition and Feeds, 2016
he present work aimed to investigate the growth performance, meat omega-3 (n-3) and omega 6 (n-6) fatty acids, immunological responses, lipid peroxide and the antioxidative status resulting from supplemented linseed oil diets of growing V-line rabbits with two levels of pomegranate peel (POM) during summer season from June to August. Forty-eight growing V-line rabbits of both sexes, 7 weeks old, with initial weight of 982.75 ± 15.85 g were used in the present experiment. Rabbits were randomly distributed to four groups of 12 rabbits each. Each group was further subdivided into 4 replicates of 3 rabbits. Group 1 fed a pelleted basal diet with standard components and served as control group, group 2 fed a pelleted diet with 2% linseed oil, group 3 fed a pelleted diet containing 2% linseed oil with 0.75 % POM, group 4 fed a pelleted diet containing 2% linseed oil with 1.5 % POM. The obtained results showed that the different treatments had insignificant effect on final body weight and daily body weight gain, however, significant (P≤0.05) decrease in feed intake and significant (P≤0.05) improvement in feed conversion were recorded in comparison with the control. Results for pre-slaughter weight, percentage of hot carcass and liver were insignificantly affected by different treatments in comparison with control. The different treatments showed significant decrease in the abdominal fat and the muscle total cholesterol in comparison with the control group. The n-3 fatty acids were significantly (P≤0.05) increased by feeding linseed oil, linseed oil plus pomegranate peel, while the n-6 fatty acids were decreased significantly by feeding linseed oil and linseed oil plus pomegranate peel in comparison with control. Hematological parameters and antibody titers against sheep red blood cells (SRBCs) were insignificantly affected by different treatments. While, specific IgG was significantly improved in all experimental groups compared with control. Serum total lipids, low density lipoprotein and malondialdehyde (MDA) were significantly (P ≤ 0.05) decreased due to different experimental diets in comparison with the control group. Total cholesterol was decreased but triglycerides were increased in animals received linseed oil in their diet in comparison with control. High density lipoprotein concentration, HDL/LDL ratio and total antioxidant capacity were significantly (P ≤ 0.05) increased by different experimental treatments as compared with the control group, with one exception that TAC was insignificantly increased than in control by receiving linseed oil in the diet. It is concluded that dietary supplementation of linseed oil plus POM in growing rabbit's diets had a beneficial effect on the composition of the meat lipid fraction by increasing the concentration of n-3 fatty acids and improving the feed conversion, blood serum lipid profile and antioxidant status.
Animals
This experiment was conducted to investigate the effect of different dietary fatty acids (saturated or polyunsaturated fatty acids) supplementation on the oxidative status and intestinal morphology of adult rabbits (Oryctolagus cuniculus). Twenty-four “slovenska kunka” rabbits were randomly assigned to two different dietary treatments (12 rabbits per treatment) and fed the experimental diets between 80 and 102 days of age. The palm fat (PALM) diet with 6% palm fat and linseed (LINSEED) diets with 6% linseed oil were used. To evaluate the oxidative status of rabbits, the malondialdehyde concentration in urine and plasma and concentration of water and lipid soluble antioxidants in plasma were measured. The antioxidative capacity of the gastrointestinal tract was evaluated by measuring concentration of water and lipid soluble antioxidants in tissues and contents of the intestine. The histological structure of the small intestine and caecum was analyzed via histomorphometric analysis. N...
Bioscience, …, 2004
Hazelnut oil (HO) is rich in monounsaturated fatty acids and antioxidants. We wanted to investigate the effect of HO on lipid levels and prooxidant-antioxidant status in rabbits fed a high-cholesterol (HC) diet. An HC diet caused significant increases in lipids and lipid peroxide levels in the plasma, liver, and aorta together with histopathological atherosclerotic changes in the aorta. Glutathione levels, glutathione peroxidase, and glutathione transferase activities decreased significantly, but superoxide dismutase activity and vitamin E and C levels remained unchanged in the livers of rabbits following HC diet. HO supplementation reduced plasma, liver, and aorta lipid peroxide levels and aorta cholesterol levels together with amelioration in atherosclerotic lesions in the aortas of rabbits fed an HC diet, without any decreasing effect on cholesterol levels in the plasma or liver. HO did not alter the antioxidant system in the liver in the HC group. Our findings indicate that HO reduced oxidative stress and cholesterol accumulation in the aortas of rabbits fed an HC diet.
Nutrition Research, 2002
The effect of linseed oil and ␣-tocopheryl acetate on fatty acid composition and susceptibility to oxidation in pig liver was studied. The ␣-tocopherol content of liver from pigs fed diets supplemented with ␣-tocopheryl acetate (200 mg/kg feed) was significantly greater (P Ͻ 0.001) than that of liver from pigs receiving a basal diet of 20 mg/kg feed. The concentration of C12:0, C16:0, C18:0 and total saturated fatty acids was higher in liver lipids from pigs fed the control diet than in the other groups (P Ͻ 0.05). No effect of dietary linseed administration was observed on monounsaturated fatty acid concentration. Adding linseed oil to pig diets either as the only source of dietary fat or in combination with olive oil produced a higher (P Ͻ 0.0001) C18:3 (n-3), C20:4 (n-3) and C22:5 (n-3) concentration in liver lipids. However, no significant effect of dietary treatment was observed on C20:5 (n-3) and C22:6 (n-3). For C20:4 (n-3), a marked effect of dietary linseed concentration was observed, while for C22:5 (n-3) there were no differences between pigs receiving a moderate or a high dietary level of linseed oil. Oxidation was higher in hepatic tissue homogenates from pigs fed linseed oil-enriched diets (P Ͻ 0.05), but 200 mg/kg dietary ␣-tocopheryl acetate supplementation reduced oxidation, reaching similar values to those of the control group.
Effect of Dietary Fats on Oxidative-Antioxidative Status of Blood in Rats
Journal of Clinical Biochemistry and Nutrition, 2010
This study was performed to examine the effect of different fat sources, lard, sunflower oil (SO), and fish oil (FO) in high-fat and low-fat diet on reactive oxygen species generation by blood phagocytes, glutathione redox status in erythrocytes, and total plasma antioxidant ability in rats. Whole blood chemiluminescence (CL) did not differ between three low-fat fed groups. However, baseline and phorbol myristate acetate (PMA)-stimulated CL in blood of high-lard fed rats were lower than in low-lard and high-SO fed animals. Phagocyte-stimulated oxidative burst was higher in rats fed high-SO diet than in those fed low-SO and high-FO diets. The highest level of oxidize glutathione (GSSH), the lowest reduce glutathione (GSH)/GSSG ratio in erythrocytes, and the highest plasma activity to reduce ferric ions were observed in rats fed both diets contaning linoleic acid-rich sunflower oil compared to animals fed the corresponding energy from other fats. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of plasma was lower in high-lard and high-FO fed rats compared to the corresponding low-fat diets, and the lowest in low-FO fed rats among low-fat fed animals. We presume from our results that linoleic acid may have dual effect, prooxidative in blood cells but maintaining total antioxidant plasma ability.
Small Ruminant Research, 2012
Several investigations have studied the influence of polyunsaturated fatty acids on the immune function of ruminants but many contradictory observations have been reported in this field. The aim of this study was to evaluate the influence of an extruded linseed dietary supplementation on the immune response and oxidative stress in the transition dairy ewe. The experiment was performed using 58 Sarda pluriparous ewes, 42 pregnant (T) and 16 non-pregnant and non-lactating (NP). Both groups were fed an isoenergetic and isonitrogenous diet from 60 d before to 15 d after lambing of the T ewes. Within each physiological state, one sub-group received a commercial pelleted concentrate (CTR) and the other an extruded linseed-enriched (10 g/100 g) concentrate (EL). Blood samples were collected from all animals at −28, −21, −14, −7, +3 and +15 d to parturition, based on expected date of lambing of the T ewes, to evaluate the innate immunity (serum lysozyme, haemolytic complement and bactericidal activity), the acquired immunity and the oxidative stress. The dietary supplementation with extruded linseed did not markedly influence the innate immunity response of the animals. The antibody production against Salmonella enterica subsp. enterica serovar Abortusovis was shown to be increased by the linseed-enriched diet in the T ewes (2.37 vs. 2.19 log 10 for the T and the NP ewes, respectively). As for the cell-mediated immunity, no differences between dietary treatments were observed. The extruded linseed-enriched diet was able to induce a transitory overproduction of oxidants in the NP ewes, whereas no effects were observed in the T ewes. In conclusion, under the adopted experimental conditions, the use of extruded linseed in the diet did not greatly affect the oxidative state and the immune response of the transition ewe.
Tropical Animal Health and Production, 2019
It is known that using antibiotics by way of growth promoters has harmful side effects on both animals and consumer health due to cross-resistance among pathogens and residues in meat. Using antibiotic to promote growth has been banned in the most countries for this reason; so, we must search for feed additives alternative in animal production. One of them is the black seed (Nigella sativa). Fiftyfour growing V-line unsexed rabbits, 4 weeks of age, with an average weight of 776.7 g were randomly allocated to one of three treatments (n = 18): rabbits fed the basal diet (control), rabbits supplemented with either 300 or 600 mg of Nigella sativa seed (NSS)/kg diet. At 12 weeks of age, NSS supplementation significantly improved final body weight, weight gain, feed conversion ratio (FCR), and performance index with significantly reduced feed consumption. Addition of 300 and 600 mg NSS/kg diet resulted in a significant increase in the number of RBCs and WBCs as compared to basal diet group. Compared to controls, NSS supplementation significantly stimulated the IgG and IgM immune responses of rabbits, significantly reduced serum total lipids, triglycerides, and low-density lipoprotein, and significantly increased the high-density lipoprotein concentration. All NSS treatments significantly increased the observed blood total antioxidant capacity and significantly decreased the malondialdehyde values, compared to the basal diet group. In conclusion, the results displayed that addition of NSS in rabbit diets improved productive performance, blood lipid profile, immunity, and antioxidant status, and supplementation with 600 mg/kg NSS was more effective than 300 mg/kg NSS seed supplementation.
Several vegetable oils have been associated with beneficial or harmful effects on the cardiovascular system which arises from the level of saturation or unsaturation of such oils. A total of 20 rats were used for the experiment for a period of 21days. The rats were grouped into four according to the feed given to them which consisted of a normal rat feed control group(group A), formulated ration of rat feed and extra virgin olive oil group (group B), formulated ration of rat feed and canola oil group (group C), and formulated ration of rat feed and sunflower oil group (group D), all in a ratio of 4(feed):1(oil). At the end of the experiment, the blood was collected by cardiac puncture. The blood was collected in both plain bottles and EDTA anti-coagulatant bottles for analysis. The result showed that except for group B, all the groups showed significant increase (P<0.05) between their mean initial and final weights.The catalase result for olive oil, canola oil and sunflower oil were 0.293±0.075IU,0.429±0.147IU and 0.527±0.022IU respectively, but were not significantly different (P>0.05) when compared to the catalase level in the control group. The superoxide dismutase activity (SOD) of rats fed with canola oil, sunflower oil as well as the control group were 0.037±0.022 IU, 0.043±0.021IU and 0.014±0.002 IU respectively which were higher than those fed with olive oil (0.012±0.007IU), but there was also no significant difference when the superoxide dismutase activity was compared in all the rat groups.There was no significant difference (P>0.05) in bothErythrocyte Sedimentation Rate (ESR) and Malondialdehyde Activity (MDA) levels in all the groups. Group C had the highest MDA levels of 0.324±0.085 µM which was higher than that of the control (group A) which had MDA levels of 0.288±0.053µM; Group B had the lowest MDA levels of 0.188±0.043 µM; Group D had the highest ESR levels of 3.667±0.667mm/hr which was above that of the control which had ESR levels of 2.000±0.577 mm/hr; Group B had the lowest ESR levels of 1.667±0.333mm/hr. This suggests that although there was no significant difference in the lipid peroxidation and inflammatory parameters investigated there are still some concerns about the type of oils that we consumewhich can predispose our systems to lipid peroxidationand inflammation. Monounsaturated oil (olive oil) seemed to be better in terms of the parameters investigated.
Pakistan Journal of Biological Sciences, 2011
The objective of the study was to evaluate the effects of different vegetable oils [red palm olein (RPO), palm olein (PO), corn oil (CO) and coconut oil (COC)] on lipid profile in rat. Sixty six Sprague Dawley male rats were randomly divided into eleven groups of 6 rats per group and were treated with 15% concentrations of RPO, PO, CO and COC for 4 and 8 weeks. Rats in control group were given normal rat pellet only while in treated groups 15% of additional vegetable oils were given. The results at 4 weeks showed a decline in Low Density Lipoprotein Cholesterol (LDL-C) values at RPO and PO groups whereas in CO and COC groups the LDL-C were increased compared to the control group. The High Density Lipoprotein Cholesterol (HDL-C) values increased in RPO and PO groups whereas it was declined in CO and COC groups compared to the control group. At 8 weeks, there was no significant difference (P ≥ 0.05) in HDL-C of rats treated with vegetable oils compared to the control group. However, the LDL-C in RPO and PO was significantly decreased (P ≤ 0.05) in the LDL-C and there was no significant difference (P≥0.05) for CO and COC groups compared to the control groups. The mean value of the LDL-C after 8 weeks in the control group, RPO, PO, CO, and COC groups were 66.1 mg/dl, 31.9 mg/dl, 41.1 mg/dl, 50.41 mg/dl and 54.31 mg/dl respectively. There was significant decreased (P ≤ 0.05) in the total cholesterol (TC) in RPO group for 4 weeks compared to the control group while the TC in PO, CO and COC were within the normal range. The results of TC in all treated rats for 8 weeks were within the normal range. There was no significant difference in TC of rats treated with vegetable oils compared to the control group. Triglycerides (TG) in all treated rats for 4 weeks were within the normal range whereas the TG in RPO, PO and CO groups for 8 weeks were significant increase (P ≤ 0.05) compared to the control group but there was no significant difference between the control group and COC group.