Cannabinoids Analysis of Laser-Microdissected Trichomes of Cannabis sativa L. by LC-MS and Cryogenic NMR (original) (raw)

Abstract

Trichomes of Cannabis sativa have been reported as the main site of cannabinoids production. A comprehensive study of cannabinoids was performed on capitate-stalked and capitate-sessile trichomes, and on capitate-stalked glands and stems harvested by laser microdissection (LMD) during flowering time (week 4 -8). LC-MS and cryogenic NMR analysis were used for qualitative and quantitative assessment of cannabinoids in the collected cells.  9 -tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and cannabigerolic acid (CBCA) were identified as the major constituents in the all tested samples, while  9 -tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabigerol (CBG) were present in less quantity. Cannabichromene (CBC) and cannabinol (CBN) were detected as minor compounds only in intact capitate-stalked trichomes in week 8. Based on the cannabinoids levels, discrimination of capitate-stalked and capitate-sessile trichomes at flowering time was possible. The study demonst...

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