Antibody response against c-terminal region of the Plasmodium vivax merozoite surface protein-1 in individuals from area without malária transmission (original) (raw)

2008

Abstract

lntroduction. The natural exposition for Plasmodium antigen is a factor that can contribute for acquisition of antibodies. The 19kDa C-terminal region of me merowite surface protein 1 (MSPl19) is a highly immunogenic conserved region of Plasmodium vivax and one of the most promising vaccine candidates against the erythrocytic forms of malaria. In the past reaIS, we had studied several immuno-epidemiological aspects of me malaria in individuaIs living in areas ofstable and exclusive transmission of P vivax in the norm of Brazil, but till this moment we do not have data available abolir the acquisition of these antibodies in samples from individuaIs mat reside in areas where the cases of malaria are not reported. Objective. The aim of this study was to analyze the antibody response among individuaIs living in areas without transmission of P vivax Material and Methods. We had produced arecombinant protein containing me His6 tag in fusion with P vivax MSPl19 in order to test and analyze antigenicity. The antibodies IgG against His6-MSPl19 were evaluated by ELISA in sera from 259 individuaIs living in Sucuriju island, located in Amapá state. ResuIts. The percentage of sera that recognized me recombinant protein was 14.67% (38/259). The concentration of antibodies was low. The mean of reactivity index was 1.66 j: 0.87. In the population examined, 250 individuaIs related never have had malaria. Among the exposed to parasite (9 individuaIs), only 2 were positive for IgG aritibodies that recognized the recombinant protein. The frequency of response was low and in cases of previous malaria episodes, only few cases were positive for detection of these specific antibodies. Conclusion. We observed that IgG antibodies response against His6-MSPl19 was detected in individuaIs that reside in area where malaria transmission was never demonstrated and that these individuaIs were exposed in anomer moment to parasite. These re$Ults shown mat MSPl19 is immunogenic after infection by P vivax but the antibodies response have an rapid decay and the re-exposition is important for a persistent antibodies response. Financial support: PETROBRAS/PIATAM-MAR, CNPq and FAPESPA.

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