Phytochemical and Biological Investigations of Flacourtia Cataphracta Roxb. Cultivated in Egypt (original) (raw)

2006, Bulletin of Pharmaceutical Sciences

n-hexane, chloroform and ethyl acetate respectively. Each concentrated fraction was subjected to TLC followed by isolation, purification and identification of the available constituents. Fourteen compounds were isolated and identified by different spectral tools (UV, IR, 1 H-NMR, 13 C-NMR, MS) and comparison with corresponding literature data. -amyrin (1), -amyrin (2), and a mixture of -sitosterol and stigmasterol (3) were isolated from nhexane fraction of both leaves and stem bark. 2-Oxo-18benzolyloxy-13(16), 14-tetrahydrocleroda-3-ene (4), 3--acetoxy-D:A friedo oleanan-27,16-lactone (5), 4,4'-dihydroxychalcone (6), apigenin (7) and kampferol (8) were isolated from chloroform fraction of the stem bark. -Sitosterol-3-O--D-glucoside (9), 5-Ocaffeoylquinic acid (10) were isolated from the ethyl acetate fraction of the leaves in addition to vanillin (11), benzoic acid (12), protocatechuic acid (13) and flacourtin (14) from the ethyl acetate fraction of the stem bark. The different leaf extracts were subjected to biological study which revealed that n-hexane, ethyl acetate and methanol fractions of Flacourtia cataphracta Roxb. are safe to be used as antidiarrheal, anti-inflammatory and antipyretic drug. leaves, stem and stem bark of the titled plant. 23 EXPERIMENTAL General experimental procedures 1-Melting points are uncorrected and measured by Koffler hot stage microscope type (ESP, Boctius M). 2-UV spectra were measured in methanol and different ionizing and complexing agents using UVidec-320 spectrophotometer (JAS CO, Japan). 3-Schimadzu infra red-470 spectrophotometer (Japan) was used for measuring IR spectra as KBr discs. 4-1 H-NMR and 13 C-NMR were recorded on JEOL TNM-LA 400 and 500 spectrophotometers using TMS as an internal standard. 5-Column chromatography was performed with silica gel 60 (E-Merck), Develosil Lop ODS (30-50 µ, Nomura Chemicals). 6-Silica gel 60 G F 254 and precoated aluminium sheets to silica gel 60 G F 254 (E-Merck) were used for TLC purposes. 7-The spots were visualized by UV lamp (254, 366 nm, VL, 6LC, Marinc Lavalec-Codex, France) and sprayed with 10% H 2 SO 4 , 5% AlCl 3 or FeCl 3. 8-Authentic samples were obtained from Department of Pharmacognosy, Faculty of Pharmacy, Assiut University. 9-Solvent systems: In -Hexane-EtOAc (9 : 1). II-CHCl 3-MeOH (9.5 : 0.5).