Anal HPV 16 and 18 viral load: A comparison between HIV-negative and -positive MSM and association with persistence (original) (raw)

Does anal HPV viral load explain the difference in anal HPV persistence between HIVnegative and-positive men who have sex with men (MSM)? Methods MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010-2011. Anal self-swabs were collected every 6 months and genotyped (SPF10-PCR-DEIA-LIPA25system). HPV16 and HPV18 load was determined with a type specific quantitative (q)PCR, and compared between HIV-negative and-positive men using ranksum test. Persistence was defined as ≥3 positive samples for the same HPV-type. Determinants of persistent HPV16/18 infection and its association with HPV16/18 load were assessed with logistic regression. Results Of 777 recruited MSM, 54 and 22 HIV negative men were HPV16 and HPV18 positive at baseline, and 64 and 39 HIV-positive MSM. The geometric mean titer (GMT) of HPV16 was 19.6 (95%CI 10.1-38.0) and of HPV18 8.6 (95%CI 2.7-27.5) DNA copies/ human cell. HPV16 and HPV18 load did not differ significantly between HIV-negative and-positive MSM (P=0.7; P=0.8, respectively). In multivariable analyses HPV16 load was an independent determinant of HPV16 persistence (OR 1.8, 95%CI 1.3-2.4). Conclusion No difference in anal HPV viral load was found between HIV-positive and HIVnegative MSM. HPV 16/18 viral load is an independent determinant of type-specific persistence.